| 1 | Expression of miR-15/107 Family MicroRNAs in Human Tissues and Cultured Rat Brain Cells显示文摘The miR-15/107 family comprises a group of 10 paralogous microRNAs(miRNAs),sharing a 50AGCAGC sequence.These miRNAs have overlapping targets.In order to characterize the expression of miR-15/107 family miRNAs,we employed customized TaqMan Low-Density micro-fluid PCR-array to investigate the expression of miR-15/107 family members,and other selected miRNAs,in 11 human tissues obtained at autopsy including the cerebral cortex,frontal cortex,primary visual cortex,thalamus,heart,lung,liver,kidney,spleen,stomach and skeletal muscle.miR-103,miR-195 and miR-497 were expressed at similar levels across various tissues,whereas miR-107 is enriched in brain samples.We also examined the expression patterns of evolutionarily conserved miR-15/107 miRNAs in three distinct primary rat brain cell preparations(enriched for cortical neurons,astrocytes and microglia,respectively).In primary cultures of rat brain cells,several members of the miR-15/107 family are enriched in neurons compared to other cell types in the central nervous system(CNS).In addition to mature miRNAs,we also examined the expression of precursors(pri-miRNAs).Our data suggested a generally poor correlation between the expression of mature miRNAs and their precursors.In summary,we provide a detailed study of the tissue and cell type-specific expression profile of this highly expressed and phylogenetically conserved family of miRNA genes. | Wang-Xia Wang Robert J.Danaher Craig S.Miller Joseph R.Berger Vega G.Nubia Bernard S.Wilfred Janna H.Neltner Christopher M.Norris Peter T.Nelson | 2014 | Genomics, Proteomics & Bioinformatics2014，12，1： | 9 |
| 2 | Radiation-induced oral mucositis hamster model using a linear accelerator enhances clinical relevance of preclinical studies for treatment strategy investigation显示文摘Translational animal models for oral mucositis(OM)are necessary to simulate and assess the bioclinical effects and response in humans.These models should simulate high levels of radiation exposure that leads to oxidative stress and inflammatoryinitiated tissue changes.Hamster models have been extensively studied to observe pathological effects of radiation exposure and help in the development of effective treatments.To successfully evaluate the potential for treatment regimens with consistency and relevance,a radiation-induced OM hamster model was developed using a clinical linear accelerator utilized by cancer patients daily.The dose exposure to the isolated,everted cheek pouch of a hamster,as well as the progression of injury,proinflammatory marker,histological,and elasticity analyses of the buccal pouch were conducted to verify replicability and reproducibility of the injury model.The findings from this model demonstrated its ability to consistently induce injury and resolution over 28 days using an acute dose of 60 Gy.This model was developed to enhance clinical relevance when evaluating potential efficacious treatments and can now be utilized in efficacy studies to better evaluate developed therapeutics in a preclinical model that is easy to translate to clinical studies.. | Carolyn T.Jordan Emily M.Bradford Dennis C.Cheek Mahesh Kudrimoti Craig S.Miller Molly H.Smith J.Zach Hilt Thomas D.Dziubla | 2021 | Animal Models and Experimental Medicine2021，4，1： | 1 |
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