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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | New directions in management accounting research 显示文摘 | Atkinson A A Balakrishnan P B Cote J M Grout T M Roberts H Ulan E Wu A | | Journal of Management Accounting Research0，997，： | 1 |
| 3 | Relaxation of Crystals with the Quasi-Newton Method显示文摘 | Pfrommer B G Cote M Louie S G | 1997 | Journal of Computational Physics1997，131，1： | 1 |
| 4 | Analysis of soil wetting and solute transport in subsurface trickle irrigation显示文摘 | Cote Claire M Bristow Keith L Charlesworth pllilip B | 2003 | Irrigation Science2003，22，3： | 1 |
| 5 | Laserpulse heating of gun bore coatings显示文摘 | COTE P J KENDALL G TODARO M E | 2001 | Surface CoatingTechnology2001，，： | 1 |
| 6 | Immersed membranes activated sludge process applied to the treatment of municipal wastewater显示文摘 | COTE P BUISSON H PRADERIE M | 1998 | Water Sci Technol1998，38，45： | 1 |
| 7 | The GDPAL region of the pre-S1 envelope protein is important for morphogenesis of woodchuck hepatitis virus显示文摘 | Yu M Emerson SU Cote P | 1998 | Hepatology1998，27，5： | 1 |
| 8 | Age-related differences in inflammatory markers in men:contribution of visceral adiposity显示文摘 | Cartier A Cote M | 2009 | Metabolism2009，58，10： | 1 |
| 9 | The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chronic obstructive pulmonary disease 显示文摘 | CELLI B R COTE C G MARIN J M | 2004 | N Engl J Med2004，350，10： | 1 |
| 10 | The body-mass index,airflow obstruction,dyspnea,and exercise capacity index in chronic obstructive pulmonary disease显示文摘 | Celli BR Cote CG Marin JM Casanova C Montes de Oca M Mendez RA Pinto Plata V Cabral HJ | 2004 | N Engl J Med2004，350，10： | 1 |
| 11 | Detection of occult bone marrow micrometastases in patients with operable lung carcinoma显示文摘 | Cote R J Mllar T Ormerod M G | 1995 | Ann Surg1995，222，4： | 1 |
| 12 | The body mass index, airflow capacity inden in chronic obstructive pulmonary disease显示文摘 | CELLI N M COTE C G MARIN J M | 2004 | N England J Med2004，350，： | 1 |
| 13 | The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chron- ic obstructive pulmonary disease 显示文摘 | Ce|li B R Cote C G Marin J M | 2004 | N Engl J Med2004，350，10： | 1 |
| 14 | Gun boreerosion mechanisms revisited with laser pulse heating显示文摘 | COTE P J TODARO M E KENDALL G | 2003 | Surface and Coatings Technology2003，163，： | 1 |
| 15 | Relaxation of crystals with the quasi-newton method显示文摘 | Pfrommer B G Cote M Louie S G | 1997 | Journal of Computational Physics1997，131，： | 1 |
| 16 | The body-mass index, airflow obstruction, dyspnea, and exercise capacity index in chronic obstructive pulmonary disease 显示文摘 | Celli B Cote C G Marin J M | 2004 | N Engl J Med2004，350，10： | 1 |
| 17 | Regular adult aspirin use decreases the risk of non-small cell lung cancer among women 显示文摘 | VAN DyKE A L COTE M L PRYSAK G | 2008 | Cancer Epidemiol Biomarkers Prey2008，17，1： | 1 |
| 18 | Studies on reac- tivity of tumor and normal cells to a wheat germ agglutinin 显示文摘 | AUB J C SANFORD B H COTE M N | 1965 | Proc Natl Acad Sci USA1965，54，2： | 1 |
| 19 | Corrosion of low carbon steel by microorganisms from the ' pigging' operation debris in water injection pipelines 显示文摘 | COTE C ROSAS O SZTYLER M | 2014 | Bioelectrochemistry2014，97，： | 1 |
| 20 | New developments in the evolution and application of the WHO/IPCS framework on mode of action/species concordance analysis 显示文摘 | MEEK M E BOOBIS A R COTE I | 2014 | J Appl Toxico12014，34，： | 1 |
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