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| 1 | Testicular expression of survivin and human telomerase reverse transcriptase(hTERT)associated with spermatogenic function in infertile patients显示文摘瞄准:与改变精子发生的功能在人的睾丸描绘 survivin, apoptosis (国际星际航空联合会) 的一个禁止者,和人的 telomerase 颠倒 transcriptase (hTERT ) 的 coexpression。方法:survivin mRNA 和 hTERT mRNA 的抄本层次用即时反向抄写的聚合酶链反应(RT-PCR ) 与有缺点的精子发生(n=28 ) 在正常睾丸(n=11 ) 和睾丸被决定。组织学的病情的检查根据一个修改 Johnsen 分数被执行。结果:survivin 和 hTERT 的表情在正常精子发生在 96.8 和 709 的中部的层次是最高的并且随减数分裂后精子发生的拘捕(n=10 ) 在睾丸落下到 53.3 和 534。在严重精子发生的失败( n=18 ), survivin 表示缺乏大多数标本( n=16 ),而阴囊的 hTERT 表示的至少底层在 premeiotic 精子发生的拘捕( n=7 )与 73 的规范的表情大部分是可检测的并且 45 在有 Sertoli 房间唯一的症候群( SCOS )( n=3 )的病人。survivin 和 hTERT 表情与一个进行的 Johnsen 分数(为 trend=0.001 的 P ) 增加了。结论:尽管 survivin 和 hTERT 与精子发生的功能被相关,他们在不肥沃的病人的睾丸显示出不同表示模式。这些调查结果在在 meiotically 划分生殖细胞建议 survivin 的占优势的表情的啮齿类动物睾丸从研究证实结果。 | Steffen Weikert Frank Christoph Wolfgang Schulze Hans Krause Carsten Kempkensteffen Martin Schostak Kurt Miller Mark Schrader | 2006 | Asian Journal of Andrology2006，8，1： | 8 |
| 2 | Improvement of quantitative testing of liver function in patients with chronic hepatitis C after installment of antiviral therapy显示文摘AIM: To investigate if and to what extent antiviral therapy influenced a broad panel of quantitative testing of liver function (QTLF).METHODS: Fifty patients with chronic hepatitis C were either treated with interferon (n = 8), interferon/ribavirin (n = 19) or peg-interferon/ribavirin (n = 23). Quantitative testing of liver function, including aminopyrine breath test (ABT), galactose elimination capacity (GEC), sorbitol clearance (SCI) and indocyanine green clearance (ICG)was performed before and 3 mo after initiation of antiviral therapy.RESULTS: After 3 mo of antiviral treatment, 36 patients showed normal transaminases and were negative for HCV-RNA, 14 patients did not respond to therapy. ABT and GEC as parameters of microsomal and cytosolic liver function were reduced in all patients before therapy initiation and returned to normal values in the 36 therapy responders after 3 mo. Parameters of liver perfusion (SCI and ICG) were not affected by antiviral therapy. In the 14 non-responders,no changes in QTLF values were observed during the treatment period.CONCLUSION: ICG and SCI remained unaffected in patients with chronic hepatitis C, while ABT and GEC were significantly compromised. ABT and GEC normalized in responders to antiviral therapy. Early determination of ABT and GEC may differentiate responders from non-responders to antivJral treatment in hepatitis C. | Matthias Ocker Marion Ganslmayer Steffen Zopf Susanne Gahr Christopher Janson Eckhart G. Hahn Christoph Herold | 2005 | World Journal of Gastroenterology2005，11，35： | 8 |
| 3 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 4 | Phase Diversity Electro-optic Sampling:A new approach to single-shot terahertz waveform recording显示文摘Recording electric field evolution in single-shot with THz bandwidth is needed in science including spectroscopy,plasmas,biology,chemistry,Free-Electron Lasers,accelerators,and material inspection.However,the potential application range depends on the possibility to achieve sub-picosecond resolution over a long time window,which is a largely open problem for single-shot techniques.To solve this problem,we present a new conceptual approach for the so-called spectral decoding technique,where a chirped laser pulse interacts with a THz signal in a Pockels crystal,and is analyzed using a grating optical spectrum analyzer.By borrowing mathematical concepts from photonic time stretch theory and radio-frequency communication,we deduce a novel dual-output electro-optic sampling system,for which the input THz signal can be numerically retrieved—with unprecedented resolution—using the so-called phase diversity technique.We show numerically and experimentally that this approach enables the recording of THz waveforms in single-shot over much longer durations and/or higher bandwidth than previous spectral decoding techniques.We present and test the proposed DEOS(Diversity Electro-Optic Sampling)design for recording 1.5 THz bandwidth THz pulses,over 20 ps duration,in single-shot.Then we demonstrate the potential of DEOS in accelerator physics by recording,in two successive shots,the shape of 200 fs RMS relativistic electron bunches at European X-FEL,over 10 ps recording windows.The designs presented here can be used directly for accelerator diagnostics,characterization of THz sources,and single-shot Time-Domain Spectroscopy. | Eléonore Roussel Christophe Szwaj Clément Evain Bernd Steffen Christopher Gerth Bahram Jalali Serge Bielawski | 2022 | Light(Science & Applications)2022，11，1： | 3 |
| 5 | Myocardial Scar Visualized by Cardiovascular Magnetic Resonance Imaging Predicts Major Adverse Events in Patients With Hypertrophic Cardiomyopathy显示文摘 | Oliver Bruder Anja Wagner Christoph J. Jensen Steffen Schneider Peter Ong Eva-Maria Kispert Kai Nassenstein Thomas Schlosser Georg V. Sabin Udo Sechtem Heiko Mahrholdt | 2010 | Journal of the American College of Cardiology2010，，11： | 2 |
| 6 | Self-expanding metal stents for the palliation of malignant gastric outlet obstruction in patients with peritoneal carcinomatosis显示文摘AIM To evaluate the efficacy of self-expanding metal stents(SEMS) for the palliation of malignant gastric outlet obstruction in patients with and without peritoneal carcinomatosis(PC).METHODS We performed a retrospective analysis of 62 patients who underwent SEMS placement for treatment of malignant gastroduodenal obstruction at our hospital over a six-year period. Stents were deployed through the scope under combined fluoroscopic and endoscopic guidance. Technical success was defined as successful stent placement and expansion. Clinical success was defined as an improvement in the obstructive symptoms and discharge from hospital without additional parenteral nutrition. According to carcinomatosis status, patients were assigned into groups with or without evidence of peritoneal disease.RESULTS In most cases, obstruction was caused by pancreatic(47%) or gastric cancer(23%). Technical success was achieved in 96.8%(60/62), clinical success in 79%(49/62) of all patients. Signs of carcinomatosis were identified in 27 patients(43.5%). The diagnosis was confirmed by pathology or previous operation in 7 patients(11.2%) and suspected by CT, MRI or ultrasound in 20 patients(32.2%). Presence of carcinomatosis was associated with a significantly lower clinical success rate compared to patients with no evidence of peritoneal disease(66.7% vs 88.6%, P = 0.036). There was no significant difference in overall survival between patients with or without PC(median 48 d vs 70 d, P = 0.21), but patients showed significantly longer survival after clinical success of SEMS placement compared to those experiencing clinical failure(median 14.5 d vs 75 d, P = 0.0003).CONCLUSION Given the limited therapeutic options and a clinical success rate of at least 66.7%, we believe that SEMS are a reasonable treatment option in patients with malignant gastric outlet obstruction with peritoneal carcinomatosis. | Christoph Rademacher Matthias Bechtler Steffen Schneider Bettina Hartmann Johannes Striegel Ralf Jakobs | 2016 | World Journal of Gastroenterology2016，22，43： | 2 |
| 7 | Phase I trial of cetuximab in combination with capecitabine, weekly irinotecan, and radiotherapy as neoadjuvant therapy for rectal cancer显示文摘 | Ralf-Dieter Hofheinz Karoline Horisberger Christoph Woernle Frederik Wenz Uta Kraus-Tiefenbacher Georg K?hler Dietmar Dinter Rainer Grobholz Steffen Heeger Stefan Post Andreas Hochhaus Frank Willeke | 2006 | International Journal of Radiation Oncology Biology Physics2006，，5： | 1 |
| 8 | CAE process to eliminate powertrain noise and vibration 显示文摘 | Mattias Schneider Hans Peter Christoph Steffens | 2002 | SAE Paper2002，，： | 1 |
| 9 | HgTe量子阱中的量子自旋霍尔绝缘态显示文摘近来,理论研究预言量子霍尔效应,在外加磁场为0的情况下物质出现的一种全新的量子态可以在HgTe/(Hg,Cd)Te量子阱中实现。我们制造了低密度高迁移率的样品结构,在样品中我们可以通过外加栅极电压调节载流子从n型穿过绝缘区到p型。对于宽度d<6.3nm的薄量子阱,绝缘区在低温下具有常规的难以察觉的微小电导。然而,对于厚的量子阱(d>6.3nm),在绝缘层中也出现接近2e2/h的剩余电导,其中e是电子电荷,h是Planck常数.剩余电导与样品宽度无关,证明这是由边缘态引起的.此外剩余电导可以被微小的外加磁场所破坏.在临界厚度d=6.3nm处发生的量子相变也与磁场诱发的绝缘-金属转变互相独立.这些观察结果为量子自旋霍尔效应提供了实验证据。 | Markus Konig Steffen Wiedmann Christoph Brüne Andreas Roth Hartmut Buhmann Laurens W.Molenkamp 黄光耀 赵安 | 2007 | 中山大学研究生学刊(自然科学与医学版)2007，28，4： | 1 |
| 10 | Combined inhibitors of angiogenesis and histone deacetylase:Efficacy in rat hepatoma显示文摘AIM:To evaluate the antitumoral effect of combined inhibitors of angiogenesis and histone deacetylases in an experimental rat hepatoma model.METHODS:MH7777A hepatoma cells were injected into the liver of male Buffalo rats.After 7 d treatment with the vascular endothelial growth factor receptor antagonist PTK787/ZK222584(PTK/ZK),the histone deacetylase inhibitor MS-275,tamoxifen(TAM) and/or retinoic acid was initiated(n ≥ 8 animals/group).Natural tumor development was shown in untreated control groups(control 1 with n = 12,control 2 with n = 8).The control groups were initiated at different time points to demonstrate the stability of the hepatoma model.For documentation of possible side effects,we documented any change in body weight,loss of fur and diarrhea.After 21 d treatment,the rats were euthanized.Main target parameters were tumor size and metastasis rate.Additionally,immunohistochemistry for the proliferating cell nuclear antigen(PCNA) and TdT-mediated dUTP-biotin nick end labeling(TUNEL) assay were performed.RESULTS:The control groups developed large tumor nodules with extrahepatic tumor burden in the lung and abdominal organs(control 1:6.18 cm3 ± 4.14 cm3 and control 2:8.0 cm3 ± 4.44 cm3 28 d after tumor cell injection).The tumor volume did not differ significantly in the control groups(P = 0.13).As single agents MS-275 and PTK/ZK reduced tumor volume by 58.6% ± 2.6% and 48.7% ± 3.2% vs control group 1,which was significant only for MS-275(P = 0.025).The combination of MS-275 and PTK/ZK induced a nearly complete and highly significant tumor shrinkage by 90.3% ± 1%(P = 0.005).Addition of TAM showed no further efficacy,while quadruple therapy with retinoic acid increased antitumoral efficacy(tumor reduction by 93 ± 1%) and side effects.PCNA positive cells were not significantly reduced by the single agents,while dual therapy(MS-275 and PTK/ZK) and quadruple therapy reduced the PCNA-positive cell fraction significantly by 9.1 and 20.6% vs control 1(P < 0.05).The number of TUNEL-positive cells,markers for ongoing apoptosis,was increased significantly by the single agents(control 1:6.9%,PTK/ZK:11.4%,MS-275:12.2% with P < 0.05 vs control 1).The fraction of TUNEL-positive cells was upregulated highly significantly by dual therapy(18.4%) and quadruple therapy(24.8%,P < 0.01 vs control 1).For the proliferating(PCNA positive) and apoptotic cell fraction,quadruple therapy was significantly superior to dual therapy(P = 0.01).CONCLUSION:Combined PTK/ZK and MS-275 were highly effective in this hepatoma model.Quadruple therapy enhanced the effects microscopically,but not macroscopically.These results should be investigated further. | Marion Ganslmayer Annette Zimmermann Steffen Zopf Christoph Herold | 2011 | World Journal of Gastroenterology2011，17，31： | 1 |
| 11 | Predictors of death or myocardial infarction during follow-up after coronary stenting with the sirolimus-eluting stent. Results from the prospective multicenter German Cypher Stent Registry显示文摘 | Ralf Zahn Christian W. Hamm Steffen Schneider Uwe Zeymer Gert Richardt Malte Kelm Benny Levenson Tassilo Bonzel Ulrich Tebbe Georg Sabin Christoph A. Nienaber Jochen Senges | 2006 | American Heart Journal2006，，6： | 1 |
| 12 | Role of Bone Marrow–Derived Cells in the Genetic Control of Restenosis显示文摘 | Nicolas Langwieser Johannes B.K. Schwarz Christoph Reichenb?cher Bastian Stemmer Steffen Massberg Nicole N. Langwieser Dietlind Zohlnh?fer | 2009 | Arteriosclerosis Thrombosis and Vascular Biology2009，，10： | 1 |
| 13 | Different capabilities of morphological pattern formation and its association with the expression of differentiation markers in a xenograft model of human pancreatic cancer cell lines显示文摘 | Daniel Neureiter Steffen Zopf Arno Dimmler Sebastian Stintzing Eckhart G. Hahn Thomas Kirchner Christoph Herold Matthias Ocker | 2005 | Pancreatology . 2005 (4-5)2005，，： | 1 |
| 14 | Incidence and predictors of target vessel revascularization after sirolimus-eluting stent treatment for proximal left anterior descending artery stenoses among 2274 patients fromthe prospectivemulticenter German Cypher Stent Registry显示文摘 | Ahmed A. Khattab Christian W. Hamm Jochen Senges Ralph Toelg Volker Geist Tassilo Bonzel Malte Kelm Benny Levenson Christoph A. Nienaber Georg Sabin Ulrich Tebbe Steffen Schneider Gert Richardt | 2007 | Clinical Research in Cardiology2007，，： | 1 |
| 15 | Myocardial Scar Visualized by Cardiovascular Magnetic Resonance Imaging Predicts Major Adverse Events in Patients With Hypertrophic Cardiomyopathy显示文摘 | Oliver Bruder Anja Wagner Christoph J. Jensen Steffen Schneider Peter Ong Eva-Maria Kispert Kai Nassenstein Thomas Schlosser Georg V. Sabin Udo Sechtem Heiko Mahrholdt | 2010 | Journal of the American College of Cardiology2010，，11： | 1 |
| 16 | Ezrin Promotes Ovarian Carcinoma Cell Invasion and Its Retained Expression Predicts Poor Prognosis in Ovarian Carcinoma显示文摘 | Martin K?bel Elise Gradhand Katharina Zeng Wolfgang D. Schmitt Karen Kriese Tilmann Lantzsch Matthias Wolters Jürgen Dittmer Hans G. Strauss Christoph Thomssen Steffen Hauptmann | 2006 | International Journal of Gynecological Pathology2006，，2： | 1 |
| 17 | CAE process to eliminate powertrain noise and vibration显示文摘 | Matthias Schneider Hans-peter Lahey Christoph Steffens and Hans-Dieter Sonntag | 2002 | SAE2002，，： | 1 |
| 18 | Outcome in the elderly undergoing percutaneous coronary intervention with sirolimus-eluting stents: Results from the prospective multicenter German Cypher Stent Registry显示文摘 | Marcus Wiemer Christoph Langer Tanja Kottmann Dieter Horstkotte Christian Hamm Thomas H. Pfannebecker Ulrich Tebbe Steffen Schneider Jochen Senges | 2007 | American Heart Journal2007，，4： | 1 |
| 19 | Phase I trial of cetuximab in combination with capecitabine, weekly irinotecan, and radiotherapy as neoadjuvant therapy for rectal cancer显示文摘 | Ralf-Dieter Hofheinz Karoline Horisberger Christoph Woernle Frederik Wenz Uta Kraus-Tiefenbacher Georg K?hler Dietmar Dinter Rainer Grobholz Steffen Heeger Stefan Post Andreas Hochhaus Frank Willeke | 2006 | International Journal of Radiation Oncology Biology Physics2006，，5： | 1 |
| 20 | Influence of cyclodextrin and temperature on the kinetics of free radical polymerization of N-adamantylacrylamide in water 显示文摘 | Christopher Steffens Oliver Kretschmann Helmut Ritter | 2007 | Macromolecular Rapid Communications2007，28，5： | 1 |
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