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| 1 | miR-211 regulates the antioxidant function of lens epithelial cells affected by age-related cataracts显示文摘AIM: To investigate the effects and mechanism of miR-211 in mediating the antioxidant function of lens epithelial cells affected by age-related cataracts.METHODS: Real-time quantitative polymerase chain reaction(RT-q PCR) was used to detect miR-211 expression in the anterior lens capsules of healthy people, the anterior lens capsules of patients with age-related cataracts, and human epithelial cell line(SRA01/04) cells exposed to oxidative stress. A 2', 7'-dichloro-fluorescein diacetate(DCFH-DA) probe was used to measure the levels of endogenous reactive oxygen species(ROS) in human lens epithelial cells(h LECs) exposed to 400 μmol/L H_2O_2 for 1 h. SRA01/04 cells were transfected with either miR-211 mimics, mimic controls, miR-211 inhibitors or inhibitor controls. After 72 h, these cells were exposed to 400 μmol/L H_2O_2 for 1 h, then p53 and Bax m RNA expression were measured using RT-q PCR. p53 and Bax protein expression were also measured by Western blotting analysis. Finally, cell viability was assessed using an MTS assay.RESULTS: Compared to the control group, expression of miR-211 in the anterior lens capsules of age-related cataract patients and in SRA01/04 cells exposed to oxidative stress was significantly increased(P<0.001). Levels of endogenous ROS were significantly elevated in h LECs exposed to oxidative stress(P<0.001). Compared to the mimic control group, the h LECs in the miR-211 mimic group expressed significantly higher levels of p53 and Bax m RNA and protein while cell viability was significantly reduced(P<0.001). Conversely, p53 and Bax m RNA and protein expression were significantly reduced in the miR-211 inhibitor group as compared to the control group, while the cells in this group had much higher levels of cell viability(P<0.001).CONCLUSION: miR-211 is upregulated in the anterior lens capsules of age-related cataract patients. miR-211 decreased the antioxidative stress capacity of lens epithelial cells by upregulating p53 and Bax, while inhibiting cell proliferation and repair. This finding suggests that miR-211 may play a key role in the development of age-related cataracts. | Bo Lu Ian T. Christensen Li-Wei Ma Tao Yu Ling-Feng Jiang Chun-Xia Wang Li Feng Jin-Song Zhang Qi-Chang Yan Xin-Ling Wang | 2018 | International Journal of Ophthalmology(English edition)2018，11，3： | 4 |
| 2 | c-Met: Structure, functions and potential for therapeutic inhibition显示文摘 | Patrick C. Ma Gautam Maulik James Christensen Ravi Salgia | 2003 | Cancer and Metastasis Reviews2003，，4： | 2 |
| 3 | Validation of X-ray fluorescence spectrometry for determining osseous or dental origin of unknown material显示文摘 | Christensen AM Smith MA Thomas RM | | 0，，01： | 1 |
| 4 | C-Met: Structure, functions- and potential for therapeutic inhibition 显示文摘 | Ma PC Maulik G Christensen J | 2003 | Cancer Metastasis Rev2003，22，4： | 1 |
| 5 | Characterization of the solitazry puhnonary nodule: 18F-FDG PET versus nodule-enhancement CT 显示文摘 | Christensen JA Nathan MA Mullan BP | 2006 | A JR Am J Roentgenol2006，187，5： | 1 |
| 6 | A multicentre, random- ized, double- blinded, placebo-controlled phase HI study to investi- gate EXtending the time for Thrombolysis in Emergency Neurologi- cal Deficits ( EXTEND ) 显示文摘 | Ma H Parsons MW Christensen S | 2012 | Int J Stroke2012，7，1： | 1 |
| 7 | 显示文摘 | Moak MA Christensen MJ | 2001 | Trace Elem Res2001，79，： | 1 |
| 8 | Effects ofanoxia on megakaryocyte progenitors derived from cord bloodCD34pos cells 显示文摘 | Saxonhouse MA Rimsza LM Christensen RD | 2003 | European Journal of Haematology2003，71，： | 1 |
| 9 | Redistribution of aquaporin -2 water channels in- duced by vasopressin in rat kidney inner medullary collecting duct 显示文摘 | Marples D Knepper MA Christensen EI | 1995 | Am J Physiol1995，269，31： | 1 |
| 10 | High dietary intake of sodium selenite induces oxidative DNA damage in rat liver 显示文摘 | Wycherly BJ Moak MA Christensen MJ | 2004 | Nutr cancer2004，48，： | 1 |
| 11 | Interactions between propofol and lipid mediator receptors: inhibition of lysophosphatidate signaling显示文摘 | Rossi MA Chan CK Christensen JD | 1996 | Anesth Analg1996，83，5： | 1 |
| 12 | Character-ization of the solitary pulmonary nodule:18 F-FDG PET versus nodule-enhancement CT显示文摘 | Christensen JA Nathan MA Mullan BP | 2006 | AJR Am J Roentgenol2006，187，5： | 1 |
| 13 | c-Met:structure,functions and potential for therapeutic inhibition显示文摘 | Ma PC Maulik G Christensen J | 2003 | Cancer Metastasis Rev2003，22，4： | 1 |
| 14 | c- Met : structure, functions and potential for therapeutic inhibition显示文摘 | Ma PC Maulik G Christensen J | 2003 | Cancer Metastasis Rev2003，22，： | 1 |
| 15 | Characterization of the solitary pulmonary nodule: ^18F-FDG PET versus nodule-enhancement CT显示文摘 | Christensen JA Nathan MA Mullah BP | 2006 | AJR2006，187，： | 1 |
| 16 | Characterization of the solitary pulmonary nodule:18 F-FDG PET versus nodule- enhancement CT显示文摘 | Christensen JA Nathan MA Mullan BP | 2006 | AIR Am J Roentgenol2006，187，5： | 1 |
| 17 | Degradation of BACE by the ubiquitin-proteasome pathway 显示文摘 | Qing H Zhou W Christensen MA | 2004 | The FASEB Journal2004，18，： | 1 |
| 18 | QTc interval length and QT dispersion as predictors of mortality in patients with non-insulin-dependent diabetes显示文摘 | Christensen PK Gall MA Major-Pedersen A | 2000 | Scand J Clin Lab Invest2000，60，： | 1 |
| 19 | A randomized trial to develop criteria for administering erythrocyte transfusions to anemic preterm infants 1 to 3 months of age显示文摘 | Ross MP Christensen RD Rothstein G Koening JM Simmons MA Noble NA | 1989 | J Perinatol1989，9，3： | 1 |
| 20 | Regional cerebral blood flow and glucose utilization during hypothermia in newborn dogs 显示文摘 | Palmer C Vannucci RC Christensen MA | 1989 | Anesthesiology1989，71，5： | 1 |
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