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| 1 | Two RING-Finger Ubiquitin E3 Ligases Regulate the Degradation of SPX4, An Internal Phosphate Sensor, for Phosphate Homeostasis and Signaling in Rice显示文摘SPX-domain-containing proteins (SPXs) play an important role in inorganic phosphate (Pi) sensing,signaling,and transport in eukaryotes.In plants,SPXs are known to integrate cellular Pi status and negatively regulate the activity of Pi central regulators,the PHOSPATE STARVATION RESPONSE proteins (PHRs).The stability of SPXs,such as SPX4,is reduced under Pi-deficient conditions.However,the mechanisms by which SPXs are degraded remain unclear.In this study,using a yeast-twhybrid screen we iden.tified two RING-finger ubiquitin E3 ligases regulating SPX4 degradation,designated SDEL1 and SDEL2,which were post-transcriptionally induced by Pi starvation.We found that both SDELs were located in the nucleus and cytoplasm,had ubiquitin E3 ligase activity,and directly ubiquitinated the K^213 and K^299 lysine residues in SPX4 to regulate its stability.Furthermore,we found that PHR2,a Pi central regulator in rice,could compete with SDELs by interacting with SPX4 under Pi-sufficient conditions,which protected SPX4 from ubiquitination and degradation.Consistent with the biochemical function of SDEL1 and SDEL2,overexpression of SDEL1 or SDEL2 resulted in Pi overaccumulation and induced Pi-starvation signaling even under Pi-sufficient conditions.Conversely,their loss-of-function mutants displayed decreased Pi accumulation and reduced Pi-starvation signaling.Collectively,our study revealed that SDEL1 and SDEL2 facilitate the degradation of SPX4 to modulate PHR2 activity and regulate Pi homeostasis and Pi signaling in response to external Pi availability in rice. | Wenyuan Ruan Meina Guo Xueqing Wang Zhenhui Guo Zhuang Xu Lei Xu Hongyu Zhao Haiji Sun Chengqi Yan Keke Yi | 2019 | Molecular Plant2019，12，8： | 14 |
| 2 | Nat Microbiol:已结首次构建出小鼠肠道微生物组数据库显示文摘小鼠模型通常被广泛用于药理学和医学研究。我们都知道小鼠肠道中的微生物群落通常会对研究结果产生明显的影响,然而目前研究者们并没有获取到关于小鼠肠道中栖息细菌群落的详细信息。近日刊登在国际杂志Nature Microbiology上的一篇研究报告中。德国慕尼黑理工大学的研究者通过研究首次收集到了小鼠肠道中的多种细菌群落。同时研究者还对其进行了分裂、特性分析。最后得到了100种细菌,其中还包括15种迄今为止未知的细菌群落。 | Lu, Qiulun Yao, Yufeng Hu, Zhenkun Hu, Changqing Song, Qixue Ye, Jian Xu, Chengqi Wang, Annabel Z. Chen, Qiuyun Wang, Qing Kenneth | 2016 | 现代生物医学进展2016，16，32： | 13 |
| 3 | Signaling pathways and targeted therapy for myocardial infarction显示文摘Although the treatment of myocardial infarction(MI)has improved considerably,it is still a worldwide disease with high morbidity and high mortality.Whilst there is still a long way to go for discovering ideal treatments,therapeutic strategies committed to cardioprotection and cardiac repair following cardiac ischemia are emerging.Evidence of pathological characteristics in MI illustrates cell signaling pathways that participate in the survival,proliferation,apoptosis,autophagy of cardiomyocytes,endothelial cells,fibroblasts,monocytes,and stem cells.These signaling pathways include the key players in inflammation response,e.g.,NLRP3/caspase-1 and TLR4/MyD88/NF-κB;the crucial mediators in oxidative stress and apoptosis,for instance,Notch,Hippo/YAP,RhoA/ROCK,Nrf2/HO-1,and Sonic hedgehog;the controller of myocardial fibrosis such as TGF-β/SMADs and Wnt/β-catenin;and the main regulator of angiogenesis,PI3K/Akt,MAPK,JAK/STAT,Sonic hedgehog,etc.Since signaling pathways play an important role in administering the process of MI,aiming at targeting these aberrant signaling pathways and improving the pathological manifestations in MI is indispensable and promising.Hence,drug therapy,gene therapy,protein therapy,cell therapy,and exosome therapy have been emerging and are known as novel therapies.In this review,we summarize the therapeutic strategies for MI by regulating these associated pathways,which contribute to inhibiting cardiomyocytes death,attenuating inflammation,enhancing angiogenesis,etc.so as to repair and re-functionalize damaged hearts. | Qing Zhang Lu Wang Shiqi Wang Hongxin Cheng Lin Xu Gaiqin Pei Yang Wang Chenying Fu Yangfu Jiang Chengqi He Quan Wei | 2022 | Signal Transduction and Targeted Therapy2022，7，4： | 11 |
| 4 | Effects of intermittent loading time and stress ratio on dwell fatigue behavior of titanium alloy Ti-6Al-4V ELI used in deep-sea submersibles显示文摘Different components of deep-sea submersibles,such as the pressure hull,are usually subjected to intermittent loading,dwell loading,and unloading during service.Therefore,for the design and reliability assessment of structural parts under dwell fatigue loading,understanding the effects of intermittent loading time on dwell fatigue behavior of the alloys is essential.In this study,the effects of the intermittent loading time and stress ratio on dwell fatigue behavior of the titanium alloy Ti-6 Al-4 V ELI were investigated.Results suggest that the dwell fatigue failure modes of Ti-6 Al-4 V ELI can be classified into three types,i.e.,fatigue failure mode,ductile failure mode,and mixed failure mode.The intermittent loading time does not affect the dwell fatigue behavior,whereas the stress ratio significantly affects the dwell fatigue life and dwell fatigue mechanism.The dwell fatigue life increases with an increase in the stress ratio for the same maximum stress,and specimens with a negative stress ratio tend to undergo ductile failure.The mechanism of dwell fatigue of titanium alloys is attribute to an increase in the plastic strain caused by the part of the dwell loading,thereby resulting in an increase in the actual stress of the specimens during the subsequent loading cycles and aiding the growth of the formed crack or damage,along with the local plastic strain or damage induced by the part of the fatigue load promoting the cumulative plastic strain during the dwell fatigue process.The interaction between dwell loading and fatigue loading accelerates specimen failure,in contrast to the case for individual creep or fatigue loading alone.The dwell fatigue life and cumulative maximum strain during the first loading cycle could be correlated by a linear relationship on the log–log scale.This relationship can be used to evaluate the dwell fatigue life of Ti alloys with the maximum stress dwell. | Chengqi Sun Yanqing Li Kuilong Xu Baotong Xu | 2021 | Journal of Materials Science & Technology2021，，18： | 7 |
| 5 | The m^(6)A methylome of SARS-CoV-2 in host cells显示文摘The newly identified Severe Acute Respiratory Syndrome Coronavirus 2(SARS-CoV-2)has resulted in a global health emergency because of its rapid spread and high mortality.The molecular mechanism of interaction between host and viral genomic RNA is yet unclear.We demonstrate herein that SARS-CoV-2 genomic RNA,as well as the negative-sense RNA,is dynamically N6-methyladenosine(m^(6)A)-modified in human and monkey cells.Combined RIP-seq and miCLIP analyses identified a total of 8 m^(6)A sites at single-base resolution in the genome.Especially,epidemic strains with mutations at these identified m^(6)A sites have emerged worldwide,and formed a unique cluster in the US as indicated by phylogenetic analysis.Further functional experiments showed that m^(6)A methylation negatively regulates SARS-CoV-2 infection.SARS-CoV-2 infection also triggered a global increase in host m^(6)A methylome,exhibiting altered localization and motifs of m^(6)A methylation in mRNAs.Altogether,our results identify m^(6)A as a dynamic epitranscriptomic mark mediating the virus–host interaction. | Jun’e Liu Yan-Peng Xu Kai Li Qing Ye Hang-Yu Zhou Hanxiao Sun Xiaoyu Li Liu Yu Yong-Qiang Deng Rui-Ting Li Meng-Li Cheng Bo He Jia Zhou Xiao-Feng Li Aiping Wu Chengqi Yi Cheng-Feng Qin | 2021 | Cell Research2021，31，4： | 5 |
| 6 | AFF3-DNA methylation interplay in maintaining the mono-allelic expression pattern of XIST in terminally differentiated cells显示文摘X chromosome inactivation and genomic imprinting are two classic epigenetic regulatory processes that cause mono-allelic gene expression.In female mammals,mono-allelic expression of the long non-coding RNA gene X-inactive specific transcript(XIST)is essential for initiation of X chromosome inactivation upon differentiation.We have previously demonstrated that the central factor of super elongation complex-like 3(SEC-L3),AFF3,is enriched at gamete differentially methylated regions(DMRs)of the imprinted loci and regulates the imprinted gene expression.Here,we found that AFF3 can also bind to the DMR downstream of the XIST promoter.Knockdown of AFF3 leads to de-repression of the inactive allele of X IST in terminally differentiated cells.In addition,the binding of AFF3 to the XIST DMR relies on DNA methylation and also regulates DNA methylation level at DMR region.However,the KAP1-H3K9 methylation machineries,which regulate the imprinted loci,might not play major roles in maintaining the mono-aUelic expression pattern of XIST in these cells.Thus,our results suggest that the differential mechanisms involved in the X IST DMR and gDMR regulation,which both require AFF3 and DNA methylation. | Yue Zhang Chao Wang Xiaoxu Liu Qian Yang Hongliang Ji Mengjun Yang Manman Xu Yunyan Zhou Wei Xie Zhuojuan Luo Chengqi Lin | 2019 | Journal of Molecular Cell Biology2019，11，9： | 3 |
| 7 | Characterization and Proteomic Analysis of Novel Rice Lesion Mimic Mutant with Enhanced Disease Resistance显示文摘Lesion mmic mutants(LMMs)are plants that spontaneously form lesions without pathogeninfection or external stimulus and exhibit resistance to pathogens.Here,a rice LMM was created by ethylmethane sulfonate mutagenesis,named as hpil(hydrogen peroxide induced lesion).Diaminobenzidineand trypan blue staining showed that large amounts of H_(2)O_(2) were produced and cell death was occurredat and around the parts of lesion mimic in the rice leaves.The phenotype of hpil is controlled by a singlerecessive gene,localized at a 2 Mb interval on chromosome 2.The data suggested that hpil is a novelLMM with enhanced bacterial and fungal disease resistance,and multiple pathogenesis-related proteins(PRs)were up-regulated.The proteomes of leaves at three positions(different degrees of lesion mimicseverity)were characterized in hpil compared with its wild type plant.Differentially expressed proteinswere detected by two dimensional difference gel electrophoresis and 274 proteins were identified byMALDITOF/TOFTM.These proteins were related to metabolic process,cellular process and response tostimulus,with mostly down-regulated in hpil leaves.Many of these proteins were related to the Calvincycle,photosynthetic electron transport chain,glycolysis/gluconeogenesis and phosphonates pathways.Some resistance-related proteins including 14-3-3 proteins,OsPR10 and antioxidases such asperoxidase,superoxide dismutase and ascorbate peroxidase were up-regulated in leaves with lesionmimic.These results provide the foundation for cloning of the target gene and shed light on themechanism involved in autaimmunity of rice. | Yang Yong Lin Qiujun Chen Xinyu Liang Weifang Fu Yuwen Xu Zhengjin Wu Yuanhua Wang Xuming Zhou Jie Yu Chulang Yan Chengqi Mei Qiong Chen Jianping | 2021 | Rice science2021，28，5： | 2 |
| 8 | An efficient strategy for mining exceptions in multi-databases 显示文摘 | Zhang Shichao Zhang Chengqi Jeffrey Xu Yu | 2004 | Information Sciences2004，165，12： | 1 |
| 9 | An efficient strategy for mining exceptions in multi-databases 显示文摘 | Shichao Zhang Chengqi Zhang Jeffrey Xu Yu | 2004 | Information Siciences2004，165，12： | 1 |
| 10 | A NEW MODULATION SCHEME FOR IR-UWB COMMUNICATION SYSTEMS显示文摘Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems. | Wang Feng Xu Chengqi Zhang Yan | 2009 | Journal of Electronics(China)2009，26，4： | 1 |
| 11 | The influence of Doppler effect on information transmission in sound channel显示文摘 | Changxue R Xu Chengqi | 1984 | IEEE International Conference Acoustics Speech and Signal Processing1984，9，1： | 1 |
| 12 | Global Remote Sensing Data SubdivisionOrganization Based on GeoSOT显示文摘 | SONG Shuhua CHENG Chengqi PU Guoliang AnFengguang LUO Xu | | 测绘学报0，，： | 1 |
| 13 | An efficient strategy for mining exceptions in multi-databases显示文摘 | Zhang Shichao Zhang Chengqi Jeffrey Xu Yu | 2004 | Information Siciences2004，165，12： | 1 |
| 14 | STGI:a spatio-temporal grid index model for marine big data显示文摘Marine big data are characterized by a large amount and complex structures,which bring great challenges to data management and retrieval.Based on the GeoSOT Grid Code and the composite index structure of the MongoDB database,this paper proposes a spatio-temporal grid index model(STGI)for efficient optimized query of marine big data.A spatio-temporal secondary index is created on the spatial code and time code columns to build a composite index in the MongoDB database used for the storage of massive marine data.Multiple comparative experiments demonstrate that the retrieval efficiency adopting the STGI approach is increased by more than two to three times compared with other index models.Through theoretical analysis and experimental verification,the conclusion could be achieved that the STGI model is quite suitable for retrieving large-scale spatial data with low time frequency,such as marine big data. | Tengteng Qu Lizhe Wang Jian Yu Jining Yan Guilin Xu Meng Li Chengqi Cheng Kaihua Hou Bo Chen | 2020 | Big Earth Data2020，4，4： | 1 |
| 15 | DOCK2 regulates antifungal immunity by regulating RAC GTPase activity显示文摘Fungal infections cause~1.5 million deaths each year worldwide,and the mortality rate of disseminated candidiasis currently exceeds that of breast cancer and malaria.The major reasons for the high mortality of candidiasis are the limited number of antifungal drugs and the emergence of drug-resistant species.Therefore,a better understanding of antifungal host defense mechanisms is crucial for the development of effective preventive and therapeutic strategies.Here,we report that DOCK2(dedicator of cytokinesis 2)promotes indispensable antifungal innate immune signaling and proinflammatory gene expression in macrophages.DOCK2-deficient macrophages exhibit decreased RAC GTPase(Rac family small GTPase)activation and ROS(reactive oxygen species)production,which in turn attenuates the killing of intracellular fungi and the activation of downstream signaling pathways.Mechanistically,after fungal stimulation,activated SYK(spleen-associated tyrosine kinase)phosphorylates DOCK2 at tyrosine 985 and 1405,which promotes the recruitment and activation of RAC GTPases and then increases ROS production and downstream signaling activation.Importantly,nanoparticle-mediated delivery of in vitro transcribed(IVT)Rac1 mRNA promotes the activity of Rac1 and helps to eliminate fungal infection in vivo.Taken together,this study not only identifies a critical role of DOCK2 in antifungal immunity via regulation of RAC GTPase activity but also provides proof of concept for the treatment of invasive fungal infections by using IVT mRNA. | Xiaojian Ma Xi Tan Bingbing Yu Wanwei Sun Heping Wang Huijun Hu Yanyun Du Ruirui He Ru Gao Qianwen Peng Zhihui Cui Ting Pan Xiong Feng Junhan Wang Chengqi Xu Bin Zhu Wei Liu Chenhui Wang | 2022 | Cellular & Molecular Immunology2022，19，5： | 1 |
| 16 | Regulation of OsPRIOa Promoter Activity by Phytohormone and Pathogen Stimulation in Rice显示文摘OsPRIOa is one of the well known pathogenesis-related genes in rice,and is induced by multiple plant hormones and pathogens.However,the underlying transcriptional regulation mechanisms in response to differe nt signals and their crosstalks are still largely unknown.In order to find new players participated in the activation of OsPRIOa,we systematically analyzed the basal expression patterns as well as the expression responses of a 2.5 kb OsPRIOa promoter in rice transgenic plants after phytohormone and pathogen stimulations.In agreement with the native gene expression,the OsPRIOa promoter can drive glucuronidase(GUS)gene expressing in spots of leaf cells,leaf trichomes,lemmas and paleae,germinating embryos,calli and root tips.The leaf expression of OsPR10a::GUS was dramatically in creased upon jasm onic acid(JA)and cytoki nin(CK)treatme nts,or challe nges of the pathogen Magnaporthe grisea and Xanthomonas oryzae pv.oryzae.Thus,the OsPRIOa promoter reported here can faithfully reflect its native gene expression.The effects of several JA and CK responsive OsWRKY genes on the regulation of OsPRIOa promoter were then inspected by luciferase transient expression assay,and the JA inducible OsWRKYlO transcription factor was found as a new positive regulator of OsPRIOa.However,the key transcription factors of JA and CK signaling pathways,OsMYC2 and B-type response regulators,were not responsible for the activation of OsPRIOa promote Our findings provided new in sights into the regulation of OsPRIOa expression during plan t-hormone/pathoge n interactions,and the OsPRIOa reporter system can be useful to unravel novel regulators from both pathogen and host. | Zheng Ersong Wang Xuming Xu Rumeng Yu Feibo Zheng Chao Yang Yong Chen Yang Chen Jianping Yan Chengqi Zhou Jie | 2021 | Rice science2021，28，5： | 1 |
| 17 | THE TR-UWB RECEIVER BASED ON SPATIAL DIVERSITY显示文摘This paper presents a new Transmitted Reference (TR) Ultra-WideBand (UWB) receiver based on Spatial Diversity (SD), which employs Multi-Antenna Technology (MAT) to improve the performance of TR-UWB receiver. According to the amplitude of correlator output of every antenna branch, this paper analyzed the performances of the proposed TR-UWB receiver employing three different kinds of combination strategies, i.e., Maximum Ratio Combination (MRC), Equal Gain Combination (EGC), and Selective Combination (SC), which are different from conventional ones, and theoretically proved that the performance of EGC is better than MRC. Simulation results verify that when EGC is adopted and BER=10-3, increasing three antennas provides Signal to Noise Ratio (SNR) gain of about 3 dB in CM4 channel and SNR gain of about 2 dB in CM2 channel. | Wang Feng Xu Chengqi Zhang Yan | 2009 | Journal of Electronics(China)2009，26，5： | 0 |
| 18 | Adolescent Mouse Takes on An Active Transcriptomic Expression During Postnatal Cerebral Development显示文摘Postnatal cerebral development is a complicated biological process precisely controlled by multiple genes. To understand the molecular mechanism of cerebral development, we compared dynamics of mouse cerebrum transcriptome through three developmental stages using high-throughput RNA-seq technique. Three libraries were generated from the mouse cerebrum at infancy, adolescence and adulthood, respectively. Consequently, 44,557,729(infancy), 59,257,530(adolescence) and 72,729,636(adulthood) reads were produced, which were assembled into15,344, 16,048 and 15,775 genes, respectively. We found that the overall gene expression level increased from infancy to adolescence and decreased later on upon reaching adulthood. The adolescence cerebrum has the most active gene expression, with expression of a large number of regulatory genes up-regulated and some crucial pathways activated. Transcription factor(TF) analysis suggested the similar dynamics as expression pro?ling, especially those TFs functioning in neurogenesis differentiation, oligodendrocyte lineage determination and circadian rhythm regulation. Moreover, our data revealed a drastic increase in myelin basic protein(MBP)-coding gene expression in adolescence and adulthood, suggesting that the brain myelin may be generated sincemouse adolescence. In addition, differential gene expression analysis indicated the activation of rhythmic pathway, suggesting the function of rhythmic movement since adolescence; Furthermore,during infancy and adolescence periods, gene expression related to axon repulsion and attraction showed the opposite trends, indicating that axon repulsion was activated after birth, while axon attraction might be activated at the embryonic stage and declined during the postnatal development. Our results from the present study may shed light on the molecular mechanism underlying the postnatal development of the mammalian cerebrum. | Wei Xu Chengqi Xin Qiang Lin Feng Ding Wei Gong Yuanyuan Zhou Jun Yu Peng Cui Songnian Hu | 2014 | Genomics, Proteomics & Bioinformatics2014，12，3： | 0 |
| 19 | Response Difference and Related Discussion of Digital Fluid Records of the Beijing Wuliying Well before Strong Earthquakes显示文摘In this paper, the precursor response characteristics of digital fluid caused by the Wenchuan M8.0 and Yushu M7.1 earthquakes are studied, and the response difference of the observations of Wuliying well to the two strong earthquakes is compared. The result shows that the abnormal fluid response has a certain relationship with earthquake size and epicenter distance. The greater the earthquake, and the closer it is to the epicenter from the observatory, the more sensitive the response will be to fluid anomalies. Abnormal Helium release was first observed before both strong earthquakes in the fluid precursor observation. The release intensity is related to earthquake magnitude; the larger the magnitude, the stronger the abnormal changes. The large change in He release in a short period after the Wenchuan earthquake may be related to the continuous activity of strong aftershocks and the structural adjustment after the earthquake. Helium release increased significantly after both earthquakes, as contributed by the abnormal deep-sited Helium release. However, this process may be connected to the annual change from July to September or the hot-reservoir type gas release in the Wuliying well. In the earthquake preparation process, a large number of deep-derived Helium is released into the geothermal system and the entire composition is changed. Temporally, this gas release appears later than fault-type gas release, and the disappearance time of this anomaly is also much later. The response difference of the fluid precursor to the two strong earthquakes may be related to differences of deep structural setting and dynamic mechanism. It also shows there is a stronger correlation between Wenchuan M8.0 earthquake in the North-South Seismic Belt and North China region than the Yushu M7.1 earthquake in the internal Qinghai-Tibetan plateau. Helium gas can be a sensitive indicator for monitoring abnormal deep-gas activity of the region where the observation station is located. Hence, observation and research should be strengthened in the future. | Gao Ling Xu Ping Xing Chengqi | 2012 | Earthquake Research in China2012，26，2： | 0 |
| 20 | PERFORMANCE ANALYSIS OF TAS/MRC-BASED SCHEME IN TIME-VARYING RAYLEIGH FADING CHANNELS显示文摘This paper investigates a Multiple-Input Multiple-Output (MIMO) scheme combining Transmit Antenna Selection and receive Maximal-Ratio Combining (TAS/MRC) in time-varying Rayleigh fading channels. We first present new closed-form expressions for optimal received Signal-to-Noise Ratio (SNR),which is expressed in polynomial form. These are used to analyze ergodic capacity,outage probability and Bit Error Rate (BER) of TAS/MRC systems. Numerical results are presented to validate the theoretical analysis. | Chen Mingxue Xu Chengqi | 2010 | Journal of Electronics(China)2010，27，2： | 0 |
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