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| 1 | RosBREED:bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops显示文摘The Rosaceae crop family(including almond,apple,apricot,blackberry,peach,pear,plum,raspberry,rose,strawberry,sweet cherry,and sour cherry)provides vital contributions to human well-being and is economically significant across the U.S.In 2003,industry stakeholder initiatives prioritized the utilization of genomics,genetics,and breeding to develop new cultivars exhibiting both disease resistance and superior horticultural quality.However,rosaceous crop breeders lacked certain knowledge and tools to fully implement DNA-informed breeding—a“chasm”existed between existing genomics and genetic information and the application of this knowledge in breeding.The RosBREED project(“Ros”signifying a Rosaceae genomics,genetics,and breeding community initiative,and“BREED”,indicating the core focus on breeding programs),addressed this challenge through a comprehensive and coordinated 10-year effort funded by the USDA-NIFA Specialty Crop Research Initiative.RosBREED was designed to enable the routine application of modern genomics and genetics technologies in U.S.rosaceous crop breeding programs,thereby enhancing their efficiency and effectiveness in delivering cultivars with producer-required disease resistances and market-essential horticultural quality.This review presents a synopsis of the approach,deliverables,and impacts of RosBREED,highlighting synergistic global collaborations and future needs.Enabling technologies and tools developed are described,including genome-wide scanning platforms and DNA diagnostic tests.Examples of DNA-informed breeding use by project participants are presented for all breeding stages,including pre-breeding for disease resistance,parental and seedling selection,and elite selection advancement.The chasm is now bridged,accelerating rosaceous crop genetic improvement. | Amy F.Iezzoni Jim McFerson James Luby Ksenija Gasic Vance Whitaker Nahla Bassil Chengyan Yue Karina Gallardo Vicki McCracken Michael Coe Craig Hardner Jason D.Zurn Stan Hokanson Eric van de Weg Sook Jung Dorrie Main Cassia da Silva Linge Stijn Vanderzande Thomas M.Davis Lise L.Mahoney Chad Finn Cameron Peace | 2020 | Horticulture Research2020，7，1： | 2 |
| 2 | Cyclin E gene amplification and CDC4 mutations in endometrial carcinoma显示文摘 | Moreno-Bueno G Rodrignez-Perales S | 2003 | J Pathol2003，201，4： | 1 |
| 3 | Spectrophotometic Determination of Cysteine and/or Carbocysteine in a Mixture of Amino Acid,Shampoo,and Pharmaceutical Product Using p-Benzoquinone显示文摘 | Dimas A M Kelly C L Cassia T B V | 1999 | Talanta1999，50，5： | 1 |
| 4 | Accumulation of cytosolic glyceraldehydes-3-phosphate dehydrogenase RNA under biological stress conditions and elicitor treatments in potato显示文摘 | Laxalt A M Cassia R O Sanllorenti P M Madrid E A Andreu A B Daleo GR Conde R D Lamattina L | | 0，，： | 1 |
| 5 | APACHE II , POSSUM and ASA scores and the risk of perioperative complications in patients with oral or oropharyngeal can- cer显示文摘 | de Cassia Braga Ribeiro K Kowalski LP | 2003 | Arch Otolaryngol Head Neck Surg2003，129，7： | 1 |
| 6 | The integration of HPV- 18 DNA in cervical carcinoma显示文摘 | Corden SA Sant - Cassia LJ Easton AJ | 1999 | Mol Pathol1999，52，5： | 1 |
| 7 | Prog- nostic score in gastric cancer:the importance of a conjoint anal- ysis of clinical, pathologic, and therapeutic factors显示文摘 | Costa M L de Cassia Braga Ribeiro K Machado M A | 2006 | Ann Surg 0ncol2006，13，6： | 1 |
| 8 | Cyclin E gene (CCNE)amplification and hCDC4 mutations in endometrial carcinoma显示文摘 | Cassia R Moreno-Bueno G Rodriguez-Perales S | 2003 | Pathol2003，201，4： | 1 |
| 9 | Increase in dermal collagen fibril diameter and elastogenesis with UVB exposure:an optical and ultrastructural study in albino Balb/c mice显示文摘 | Carneiro SC Cassia Fde F Pascarelli BM | 2007 | Acta Dermatovenerol Croat2007，15，2： | 1 |
| 10 | Cardiovascular risk in vegetarians and omnivores : a comparative study 显示文摘 | Rita de Cassia Moreira de Almeida Teixeira Maria del Carmen Bisi Molina Eliana Zandonade Jose Geraldo Mill | 2007 | Arquivos Brasileiros de Cardiologia2007，89，4： | 1 |
| 11 | An increase in the concentration of abscisic acid is critical for nitric oxide- mediated plant adaptive responses to UV-B irradiation 显示文摘 | Tossi V Lamattina L Cassia R | 2009 | The New Phytologist2009，181，4： | 1 |
| 12 | Maternal serum visfatin at 11-13 weeks' gestation in pre- eclampsia显示文摘 | Ferreira AF Rezende JC de Cassia C Oliveira R | 2013 | J Hum Hypertens2013，27，4： | 1 |
| 13 | A study of reaction between quinones and thiourea: determination of thiourea in orange juice 显示文摘 | Adriana N deOliveira Henrique de Santana Cassia TBV Zaia | 2004 | J Food Composition Analysis2004，17，： | 1 |
| 14 | In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria显示文摘Yerba mate(Ilex paraguariensis A.St.-Hil.)is a species of great economic,social and environmental importance for the southern regions of Brazil,Uruguay and Argentina.Currently the most diverse products are obtained from mate leaves,including mate tea.The objective of this study was to establish shoot meristem cultures(meristematic dome and a few primordia)of elite clones and identify the endophytic bacteria present in the explants.We tested the effect of clones(F1,F2,A03 and A07),culture media(MS,1/2MS,1/4MS and WPM),cytokinins(kinetin,BA and 2iP),activated charcoal(1,2 and 3 g L^-1),and disinfecting agent(sodium hypochlorite and mercuric chloride)on in vitro establishment.F1 and F2 clones were the most responsive for shoot meristem in vitro culture.WPM medium supplemented with 8.8 lM 2iP,0.2 lM NAA and 3 g L^-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone.No phytotoxic effect of the disinfectant was observed and some meristems sprouted.The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei.To conclude,we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response. | Jessica de Cassia Tomasi Juliana Degenhardt-Goldbach Renata Lucia Grunennvaldt Germana Davila dos Santos Vania Aparecida Vicente Luziane Franciscon Cleusa Bona Marguerite Quoirin | 2019 | Journal of Forestry Research2019，30，5： | 1 |
| 15 | Combined epidermal growth factor receptor targeting with the tyrosine kinase inhibitor gefitinib (ZD1839) and the monoclonal antibody cetuximab (IMC-C225):superiority over single-agent receptor targeting显示文摘 | Matar P Rojo F Cassia R | 2004 | Clin Cancer Res2004，10，19： | 1 |
| 16 | Microporous activated carbon prepared from coconut shells using chemical activation with zinc chloride 显示文摘 | Diana C S Azevedo J Cassia S | 2007 | Microporous and Mesoporous Materials2007，100，13： | 1 |
| 17 | Comparison of the in vitro estrogenic activities of compounds from hops ( Humuhts iupulus) and red clover ( Trifolium pratense) 显示文摘 | Cassia R Overka Ping Yaoa Lucas R Chadwicka | 2005 | Agric Food Chem2005，53，16： | 1 |
| 18 | Strategic innovation and new product development in fhmily firms: An em- pirically grounded theoretical framework 显示文摘 | Cassia L De Massis A Pizzurno E | 2012 | International Journal of Entrepreneurial Behavior & Research2012，18，2： | 1 |
| 19 | Spectrophotometric determination of cysteine and/or carbocysteine in a mixture of amino acid, shampoo,and pharmaceutical products using p-benzoquinone 显示文摘 | Dimas A S Zaia Kelly C L Ribas Cassia T B V Zaia | 1999 | Talanta1999，50，： | 1 |
| 20 | Prognostic Value of Measurements of Angiogenesis in Serous Carcinoma of the Ovary显示文摘 | Julia E. Palmer Louis J. Sant Cassia Clive J. Irwin Alan G. Morris Terence P. Rollason | 2007 | International Journal of Gynecological Pathology2007，，： | 1 |
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