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5篇 您的检索式：作者名="Cassandra Long"

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		题名	作者	年代	出处	被引量
	1	A comparison of three methods of decellularization of pig corneas to reduce immunogenicity显示文摘·AIM: To investigate whether decellularization using different techniques can reduce immunogenicity of the cornea, and to explore the decellularized cornea as a scaffold for cultured corneal endothelial cells(CECs).Transplantation of decellularized porcine corneas increases graft transparency and survival for longer periods compared with fresh grafts.·METHODS: Six-month-old wild-type pig corneas were cut into 100-200 μm thickness, and then decellularized by three different methods: 1) 0.1% sodium dodecyl sulfate(SDS); 2) hypoxic nitrogen(N2); and 3) hypertonic NaCl. Thickness and transparency were assessed visually. Fresh and decellularized corneas were stained with hematoxylin/eosin(H&E), and for the presence of galactose-α1,3-galactose(Gal) and N-glycolylneuraminic acid(NeuGc, a nonGal antigen). Also, a human IgM/IgG binding assay was performed. Cultured porcine CECs were seeded on the surface of the decellularized cornea and examined after H&E staining.· RESULTS: All three methods of decellularization reduced the number of keratocytes in the stromal tissue by >80% while the collagen structure remained preserved. No remaining nuclei stained positive for Gal or NeuGc, and expression of these oligosaccharides on collagen was also greatly decreased compared to expression on fresh corneas. Human IgM/IgG binding to decellularized corneal tissue was considerably reduced compared to fresh corneal tissue. The cultured CECs formed a confluent monolayer on the surface of decellularized tissue.· CONCLUSION: Though incomplete, the significant reduction in the cellular component of the decellularized cornea should be associated with a significantly reduced in vivo immune response compared to fresh corneas.	Whayoung Lee Yuko Miyagawa Cassandra Long David K.C.Cooper Hidetaka Hara	2014	International Journal of Ophthalmology(English edition)2014，7，4：	7
	2	Distribution of Non-Gal Antigens in Pig Cornea: Relevance to Corneal Xenotransplantation显示文摘	David Cohen Yuko Miyagawa Ruhina Mehra Whayoung Lee Kumiko Isse Cassandra Long David L. Ayares David K. C. Cooper Hidetaka Hara	2014	Cornea2014，，4：	1
	3	Characterization of Porcine Corneal Endothelium for Xenotransplantation显示文摘	Seung Eun Lee Ruhina Mehra Minoru Fujita Danny S. Roh Cassandra Long Whayoung Lee James L. Funderburgh David L. Ayares David K. C. Cooper Hidetaka Hara	2013	Seminars in Ophthalmology2013，，3：	1
	4	Lrp5 and Lrp6 redundantly control skeletal development in the mouse embryo显示文摘	Kyu Sang Joeng Cassie A. Schumacher Cassandra R. Zylstra-Diegel Fanxin Long Bart O. Williams	2011	Developmental Biology2011，，2：	1
	5	Lrp5 and Lrp6 redundantly control skeletal development in the mouse embryo显示文摘	Kyu Sang Joeng Cassie A. Schumacher Cassandra R. Zylstra-Diegel Fanxin Long Bart O. Williams	2011	Developmental Biology2011，，2：	1

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