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| 1 | Probiotic metabolites from Bacillus coagulans GanedenBC30^(TM) support maturation of antigen-presenting cells in vitro显示文摘AIM:To study the effects of probiotic metabolites on maturation stage of antigen-presenting immune cells.METHODS:Ganeden Bacillus coagulans 30(GBC30) bacterial cultures in log phase were used to isolate the secreted metabolite(MET) fraction.A second fraction was made to generate a crude cell-wall-enriched fraction,by centrifugation and lysis,followed by washing.A preparation of MET was subjected to size exclusion centrifugation,generating three fractions:< 3 kDa,3-30 kDa,and 30-200 kDa and activities were tested in comparison to crude MET and cell wall in primary cultures of human peripheral blood mononuclear cell(PBMC) as a source of antigen-presenting mononuclear phagocytes.The maturation status of mononuclear phagocytes was evaluated by staining with monoclonal antibodies towards CD14,CD16,CD80 and CD86 and analyzed by flow cytometry.RESULTS:Treatment of PBMC with MET supported maturation of mononuclear phagocytes toward both macrophage and dendritic cell phenotypes.The biological activity unique to the metabolites included a reduction of CD14+ CD16+ pro-inflammatory cells,and this property was associated with the high molecular weight metabolite fraction.Changes were also seen for the dendritic cell maturation markers CD80 and CD86.On CD14dim cells,an increase in both CD80 and CD86 expression was seen,in contrast to a selective increase in CD86 expression on CD14bright cells.The co-expression of CD80 and CD86 indicates effective antigen presentation to T cells and support of T helper cell differentiation.The selective expression of CD86 in the absence of CD80 points to a role in generating T regulatory cells.CONCLUSION:The data show that a primary mechanism of action of GBC30 metabolites involves support of more mature phenotypes of antigen-presenting cells,important for immunological decision-making. | Kathleen F Benson Kimberlee A Redman Steve G Carter David Keller Sean Farmer John R Endres Gitte S Jensen | 2012 | World Journal of Gastroenterology2012，18，16： | 8 |
| 2 | Lubricantderived tribochemical films-an infra-red spectroscopic study显示文摘 | Carter R O Boulos E N | 1992 | Tribology International1992，36，： | 1 |
| 3 | A theory of finite elastic consolidation显示文摘 | CARTER J P SMALL J C BOOKER J R | 1977 | International Journal of Solids and Structures1977，13，5： | 1 |
| 4 | Epidemiology of hospitalizations and death from heat illness in soldiers显示文摘 | Carter R 3rd Cheuvront S N Williams J O | 2005 | Med Sci Sports Exerc2005，37，4： | 1 |
| 5 | Maternal health factors and early pediatric antiretroviral therapy influence the rate of perinatal HIV-1 disease progression in children显示文摘 | Abrams EJ Wiener J Carter R | 2003 | AIDS2003，17，： | 1 |
| 6 | Measuring bottleneck link speed in packet switched networks显示文摘 | CARTER R CROVELLA M | 1996 | Performance Evaluation1996，27，8： | 1 |
| 7 | Patient-maintained sedation for ERCP with a target-controlled infusion of propofol:a pilot study显示文摘 | Gillham MJ Hutchinson RC Carter R | 2001 | Gastrointest Endosc2001，54，： | 1 |
| 8 | Abdominal apoplexy: report of six cases and review of the literature 显示文摘 | Carter R Gosuey WG | 1966 | Am J Surg1966，116，3： | 1 |
| 9 | The Asian lower eyelid:a comparative anatomic study using high-resolution magnetic resonance imaging显示文摘 | CARTER S R SEIFF S R GRANT P E | 1998 | Ophthal Plast Reconstr Surg1998，14，4： | 1 |
| 10 | Freezing effects on aggregate stability affected by texture, mineralogy, and organic matter显示文摘 | Lehrsch G A Sojka R E Carter D L | 1991 | Soil Science Society of America Journal1991，55，： | 1 |
| 11 | Underwriter reputation,initial returns,and the long-run performance of IPO stocks显示文摘 | Carter R Dark F Singh A | | 0，，53： | 1 |
| 12 | Geodetic im- aging with airborne LiDAR: The Earth's surface revealed 显示文摘 | Glennie C L Carter W E Shrestha R L | 2013 | Reports on Progress in Physics2013，76，8： | 1 |
| 13 | Experimental studies of pylon-aided fuel injection into a supersonic cross flow显示文摘 | Mark R Gruber Campell D Carter Daniel R Monters | 2008 | Journal of Aerospace Power2008，5，6： | 1 |
| 14 | Was the Indosinian orogeny a Triassic mountain building or a thermotectonic reactivation event?显示文摘 | Carter A P D Clift C R | 2008 | C R Geosciences2008，340，： | 1 |
| 15 | Unilateral diaphragrnatic paralysis in spinal cord injury patients显示文摘 | Carter R E | 1980 | Paraplegin1980，18，： | 1 |
| 16 | Epidemiology and outcome of mould infections in hematopoietic stem cell transplant recipients显示文摘 | Marr K A Carter R A Crippa F | 2002 | Clin Infect Dis2002，34，： | 1 |
| 17 | Prevalence and impact of gastroesophageal reflux in adolescents with asthma: a population-based study 显示文摘 | Debley J S Carter E R Redding G J | 2006 | Pediatr Pulmonol2006，41，5： | 1 |
| 18 | Soil structural form and stability, and organic matter under cool-season perennial grasses 显示文摘 | Carter M R Angers D A Kunelius H T | 1994 | Soil Sci Soc Am J1994，58，： | 1 |
| 19 | Environmental purchasing and firm performance: An empirical investigation 显示文摘 | Carter C R Kale R Grimm C M | 2000 | Transportation Research Part E: Logistics and Transportation Review2000，36，3： | 1 |
| 20 | Preferential survival of CD4+ T lymphecytes engineered with anti-human immunodeficiency virus (HIV) genes in HIV-infectod individuals显示文摘 | Morgan RA Walker R Carter CS | 2005 | Hum Gene Ther2005，16，9： | 1 |
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