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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | A place to be: the role of clubhouses in facilitating social support 显示文摘 | CAROLAN M ONAGA E PERNICE-DUCA F | 2011 | Psychiatr MCKAY programs: Psychiatr Rehabil J2011，35，2： | 1 |
| 3 | Making Patents and Intellectual Property Work: The Asymmetrical “ Harmonization” of TRIPS 显示文摘 | CAROLAN M S | 2008 | Organization & Environment2008，21，3： | 1 |
| 4 | Cryptic bumblebee species:consequences for conservation and the trade in greenhouse pollinators显示文摘 | Williams PH An J Brown MJF Carolan JC Goulson D Huang J Ito M | | 0，，03： | 1 |
| 5 | Diabetes nurse educators, experiences of pro-viding care for women, with gestational diabetes melli-tus?from disadvantaged backgrounds 显示文摘 | Carolan M | 2013 | J Clin Nurs2013，23，910： | 1 |
| 6 | Maternal age,ethnicity and gestational diabetes mellitus显示文摘 | Carolan M Davey MA Biro MA | 2012 | Midwifery2012，28，6： | 1 |
| 7 | Advanced mater- nal age and obstetric morbidity for women giving birth in Victoria;A population-based study显示文摘 | Biro MA Davey MA Carolan M | 2012 | Aust NZJ Obstet Gynaecol2012，52，3： | 1 |
| 8 | Older maternal age and intervention in labor: a population-based study comparing old- er and younger first- time mothers in Victoria 显示文摘 | Carolan M Davey MA Biro MA | 2011 | Australia Birth2011，38，1： | 1 |
| 9 | Diabetes nurse educators experiences of pro- viding care for women,with gestational diabetes mellitus, from disadvantaged backgrounds显示文摘 | Carolan M | 2014 | J Clin Nurs2014，23，910： | 1 |
| 10 | A good midwife stands out':3rd year midwifery students' views显示文摘 | Carolan M | | 0，，： | 1 |
| 11 | Effect of recovery method on yield of bovine oocytes per ovary and their developmental competence after maturation, fertilization and culture in vitro显示文摘 | CAROLAN C MONAGHAN P GALLAGHER M | 1994 | Theriogenology1994，41，： | 1 |
| 12 | Role of clinical nurse leadership in improving patient care显示文摘 | Murphy J Quillinan B Carolan M | 2009 | Nurs Manag (Harrow)2009，16，8： | 1 |
| 13 | Interfacial effects in a two-phase partitioning bioreactor:degradation of polycyclic aromatic hydrocarbons (PAHs) by a hydrophobic Mycobacterium显示文摘 | Carolan T M Andrew J D | 2005 | Process Biochemistry2005，40，5： | 1 |
| 14 | Diabetes nurse educators' experiences of providing care for women,with gestationa] diabetes mellitus,from disadvantaged backgrounds显示文摘 | Carolan M | 2014 | Journal of Clinical Nursing2014，25，57： | 1 |
| 15 | Women's experiences of gestational diabetes self-management:a qualitative study显示文摘 | Carolan M | 2013 | Midwifery2013，29，6： | 1 |
| 16 | Diabetes nurse educators' experiences of providing care for women with gestational diabetes mellitus from disad- vantaged backgrounds显示文摘 | Carolan M | 2014 | J Clin Nurs2014，23，10： | 1 |
| 17 | ITM Syngas ceramic membrane technology for synthesis gas production显示文摘 | Christopher M Chen Douglas L Bennett Michael F Carolan | 2004 | Studies in Surface Science and Catalysis2004，147，4： | 1 |
| 18 | Effect of recovery method on yield bovine oocytes per ovary and their developmental competence after maturation, fertilization and culture in vitro 显示文摘 | Carolan C Monaghan P Gallegher M | 1994 | Theriogenology1994，41，5： | 1 |
| 19 | Advanced maternal age and adverse perinatal outcome : A review of the evidence显示文摘 | Carolan M Frankowska D | 2011 | Midwifery2011，27，6： | 1 |
| 20 | Maternal age,eth nicity and gestational diabetes mellitus 显示文摘 | Carolan M Davey MA Biro MA | 2012 | Midwifery2012，28，6： | 1 |
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