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| 1 | Effect of bombesin and neurotensin on gut barrier function in partially hepatectomized rats显示文摘AIM: To investigate the effect of regulatory peptides bombesin (BBS) and neurotensin (NT) on intestinal barrier function in partially hepatectomized rats.METHODS: Ninety male Wistar rats were randomly divided into five groups: Ⅰ (n = 10): controls, Ⅱ (n =20): sham operated, Ⅲ (n = 20): partial hepatectomy 70% (PHx), Ⅳ (n = 20): PHx+BBS (30 μg/kg/d), Ⅴ (n= 20): PHx+NT (300 μg/kg/d). Groups Ⅳ and Ⅴ were treated for 8 days before PHx and 48 h post surgery.At the end of the experiment, on day 10, intestinal barrier function was assessed by measuring endotoxin concentrations in portal and aortic blood. Tissue sections of the terminal ileum were examined histologically and villus density, mucosal thickness, mitotic activity and apoptosis in crypts were assessed. In addition, ileal mucosa was analyzed for DNA and protein content and microbiological analysis was performed in cecal contents.To estimate intestinal oxidative stress, lipid peroxidation was determined on tissue homogenates from terminal ileum.RESULTS: BBS or NT administration significantly reduced portal and systemic endotoxemia observed 48 h after partial hepatectomy. In hepatectomized rats (group Ⅲ), a trend towards induction of mucosal atrophy was observed, demonstrated by the reduction of villus density, mucosal thickness, protein content and significant reduction of DNA, while these alterations were reversed by regulatory peptides administration.This trophic effect of BBS and NT was accompanied by induction of mitoses above control levels and a significant reduction of apoptosis in intestinal crypts.Intestinal lipid peroxidation was found significantly lower in PHx group and regulatory peptides exerted an antioxidant action, further decreasing this parameter of oxidative stress. The bacterial population of E. coli and aerobic Gram (+) cocci was increased in cecal content of hepatectomized rats, while this parameter was not affected by the administration of BBS or NT.CONCLUSION: Gut regulatory peptides BBS and NT improve intestinal barrier function and reduce endotoxemia in experimental partial hepatectomy. This effect is, at least in part, mediated by their trophic,antiapoptotic, mitogenic, and antioxidant effect on the intestinal epithelium. This observation might be of potential value in patients undergoing liver resection. | Stelios F Assimakopoulos Ilias H Alexandris Chrisoula D Scopa Panagiotis G Mylonas Konstantinos C Thomopoulos Christos D Georgiou Vassiliki N Nikolopoulou Constantine E Vagianos | 2005 | World Journal of Gastroenterology2005，11，43： | 8 |
| 2 | Intestinal barrier dysfunction in cirrhosis: Current concepts in pathophysiology and clinical implications显示文摘The intestinal lumen is a host place for a wide range of microbiota and sets a unique interplay between local immune system, inflammatory cells and intestinal epithelium, forming a physical barrier against microbial invaders and toxins. Bacterial translocation is the migration of viable or nonviable microorganisms or their pathogen-associated molecular patterns, such as lipopolysaccharide, from the gut lumen to the mesenteric lymph nodes, systemic circulation and other normally sterile extraintestinal sites. A series of studies have shown that translocation of bacteria and their products across the intestinal barrier is a commonplace in patients with liver disease. The deterioration of intestinal barrier integrity and the consulting increased intestinal permeability in cirrhotic patients play a pivotal pathophysiological role in the development of severe complications as high rate of infections, spontaneous bacterial peritonitis, hepatic encephalopathy, hepatorenal syndrome, variceal bleeding, progression of liver injury and hepatocellular carcinoma. Nevertheless, the exact cellular and molecular mechanisms implicated in the phenomenon of microbial translocation in liver cirrhosis have not been fully elucidated yet. | Georgios I Tsiaoussis Stelios F Assimakopoulos Athanassios C Tsamandas Christos K Triantos Konstantinos C Thomopoulos | 2015 | World Journal of Hepatology2015，7，17： | 4 |
| 3 | Stimulation of oval cell and hepatocyte proliferation by exogenous bombesin and neurotensin in partially hepatectomized rats显示文摘AIM:To investigate the effect of the neuropeptides bombesin(BBS)and neurotensin(NT)on oval cell proliferation in partially hepatectomized rats not pretreated with a known hepatocyte inhibitor.METHODS:Seventy male Wistar rats were randomly divided into five groups:Ⅰ=controls,Ⅱ=sham operated,Ⅲ=partial hepatectomy 70%(PHx),Ⅳ=PHx+ BBS(30μg/kg per day),Ⅴ=PHx+NT(300μg/kg per day).Forty eight hours after liver resection,portal en-dotoxin levels and hepatic glutathione redox state were determined.α-fetoprotein(AFP)mRNA(in situ hybridisation),cytokeratin-19 and Ki67 antigen expression (immunohistochemistry)and apoptosis(TUNEL)were evaluated on liver tissue samples.Cells with morphological features of oval cells that were cytokeratin-19 (+)and AFP mRNA(+)were scored in morphometric analysis and their proliferation was recorded.In addition,the proliferation and apoptotic rates of hepatocytes were determined.RESULTS:In the control and sham operated groups,oval cells were significantly less compared to groups Ⅲ,ⅣandⅤ(P<0.001).The neuropeptides BBS and NT significantly increased the proliferation of oval cells compared to groupⅢ(P<0.001).In addition,BBS and NT induced a significant increase of hepatocyte proliferation(P<0.001),whereas it decreased their apoptotic activity(P<0.001)compared to groupⅢ.BBS and NT significantly decreased portal endotoxemia (P<0.001)and increased the hepatic GSH:GSSG ratio (P<0.05 and P<0.001,respectively)compared to groupⅢ.CONCLUSION:BBS and NT stimulated oval cell proliferation in a model of liver regeneration,without use of concomitant suppression of hepatocyte proliferation as oval cell activation stimuli,and improved the hepatocyte regenerative response.This peptides-induced combined stimulation of oval cell and hepatocyte proliferation might serve as a possible treatment modality for several liver diseases. | Stelios F Assimakopoulos Athanassios C Tsamandas Ilias H Alexandris Christos Georgiou Constantine E Vagianos Chrisoula D Scopa | 2011 | World Journal of Gastrointestinal Pathophysiology2011，2，6： | 2 |
| 4 | A rare etiology of post-endoscopic retrograde cholangiopancreatography pneumoperitoneum显示文摘Major complications of endoscopic retrograde cholangiopancreatography (ERCP) include pancreatitis, hemorrhage, cholangitis, and duodenal perforation. The occurrence of free air in the peritoneal cavity post- ERCP is a rare event (< 1%), which is usually the result of duodenal or ductal perforation related to therapeutic ERCP with sphincterotomy. We describe for the first time a different aetiology of pneumoperitoneum, in an 84-year-old woman with pancreatic cancer and a large hepatic metastasis, after ERCP with common bile duct stent deployment. Our patient developed, pneumoperitoneum due to air leakage from rupture of intrahepatic bile ducts and Glisson’s capsule in the area of a peripheral large hepatic metastasis. The potential mechanism underlying this complication might be post- ERCP pneumobilia and increased pressure of intrahepatic bile ducts leading to rupture of intrahepatic bile ducts in the liver metastatic mass owing to neoplastic tissue friability. This case indicates the need for close clinical and radiological observation of patients with hepatic masses (primary or metastatic) subjected to ERCP. In such patients, avoidance of excessive air insufflation during ERCP and/or placement of a nasogastric tube for bowel decompression immediately after ERCP might be a reasonable strategy to prevent such unusual complications. | Stelios F Assimakopoulos Konstantinos C Thomopoulos Sofia Giali Christos Triantos Dimitrios Siagris Charalambos Gogos | 2008 | World Journal of Gastroenterology2008，14，18： | 2 |
| 5 | Modeling turbulent reacting jets issuing into a hot and diluted coflow 显示文摘 | CHRISTO F C DALLY B B | 2005 | Combustion and Flame2005，142，1： | 1 |
| 6 | Modeling turbulent reacting jets issuing into a hot and diluted coflow显示文摘 | Christo F C Dally B B | | 0，，1： | 1 |
| 7 | Modeling turbulent reacting jets issuing into a hot and diluted coflow显示文摘 | Christo F C Dally B B | 2005 | Combustion and Flame2005，142，12： | 1 |
| 8 | Mechanical Stress Is Required for High - Level Expression of Connective Tissue Growth Factor Christ of Schild and Beat Truebl 显示文摘 | CHRISTO F SCHILD C TRUEB B | 2002 | Experimental Cell Research2002，，274： | 1 |
| 9 | Free-riding and whitewashing in peer to-peer systems显示文摘 | MICHAL F CHRISTOS P JOHN C | 2006 | IEEE Journal on Selected Areas In Communications2006，，5： | 1 |
| 10 | A CFD based combustion model of an entrained flow biomassgasifier显示文摘 | FLETCHER D F HAYNES B S CHRISTO F C JOSEPH S D | 2000 | Appl Math Model2000，24，3： | 1 |
| 11 | Critical Thinking: The Develop- ment of an Essential Skill for Nursing Students 显示文摘 | Ioanna V Christos F Evangelos C | 2014 | Acta Inform Med Aug2014，22，4： | 1 |
| 12 | Modeling Turbulent Reacting Jets Issuing into a Hot and Diluted Cofiow 显示文摘 | Christo F C Dally B B | 2005 | Combustion and Flame2005，142，11： | 1 |
| 13 | A CFD based combustion model of an entrained flow biomass gasifier 显示文摘 | Ffetcher D F Haynes B S Christo F C | 2000 | Applied Mathematical Modelling2000，24，3： | 1 |
| 14 | Modeling Turbulent Reacting Jets Issuing into a Hot and Diluted Coflow 显示文摘 | CHRISTO F C DALLY B B | 2005 | Combustion andFlame2005，42，： | 1 |
| 15 | Numerical modelling of wind and dust patterns around a full-scale paraboloidal solar dish original research article显示文摘 | Christo F C | 2012 | Renewable Energy2012，39，1： | 1 |
| 16 | Numerical modelling of wind and dust patterns around a full-scale paraboloidal solar dish显示文摘 | CHRISTO F C | 2012 | Renewable Energy2012，39，1： | 1 |
| 17 | Modeling turbulent reacting jetsissuing into a hot and diluted coflow 显示文摘 | Christo F C Dally B B | 2005 | Combustionand Flame2005，142，1： | 1 |
| 18 | Modeling turbulent reacting jets issuing into a hot and diluted coflow 显示文摘 | Christo F C Dally B B | 2005 | Combustion and Flame2005，142，12： | 1 |
| 19 | Modeling turbulent reacting jets issuing into a hot and diluted coflow 显示文摘 | Christo F C Dally B B | 2005 | Combustion and Flame2005，142，： | 1 |
| 20 | Free Riding and Whitewash- ing in Peer to Peer Systems显示文摘 | Michal F Christos P John C | 2006 | IEEE Journal on Selected Areas in Communications2006，24，5： | 1 |
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