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| 1 | α-Glucosidase immobilization on functionalized Fe_3O_4 magnetic nanoparticles for screening of enzyme inhibitors显示文摘Magnetic nanoparticles(MNPs) are widely used for the immobilization of enzyme owing to the unique properties such as good biocompatibility and rapid separation. Herein, we used Fe_3O_4 magnetic nanoparticles(Fe_3O_4 MNPs) as the carrier core with(3-aminopropyl)triethoxysilane(APTES)modification by our approach, in which a-glucosidase was stereoscopically immobilized on the surface of Fe_3O_4 MNPs via covalent binding. The result of immobilization was characterized by scanning electron microscope(SEM) and fourier transform-infrared spectroscopy(FT-IR). Then we optimized some key parameters of the immobilization reaction, including the ratio of MNPs to enzyme, GA concentration,crosslinking time and immobilization time. Moreover, under the optimal conditions, pH tolerance,thermo stability and reusability of the immobilized enzyme were investigated and compared with the free one. In order to evaluate the change of the affinity of the enzyme to its specific substrate after immobilization, the Michaelis-Menten constant(K_m) was also studied. Finally, the immobilized α-glucosidase combining with high performance liquid chromatography-tandem mass spectrometry technique(HPLC-MS/MS) was applied to screen and identify eight inhibitors from Polygonum cuspidatum extract. These results indicated that the established method had the broad prospects for biotechnological applications. | Guorong Cheng Junpeng Xing Zifeng Pi Shu Liu Zhiqiang Liu Fengrui Song | 2019 | Chinese Chemical Letters2019，30，3： | 2 |
| 2 | Prediction and Analysis on Short-term Load of Power System Based on LSTM显示文摘Effective short-term prediction of regional voltage load is of great significance to the implementation of energy saving and emission reduction policies in China.Accurate prediction of real-time demand voltage can reduce power waste and carbon emissions,make outstanding contributions to delaying global climate warming,and is conducive to global environmental protection and sustainable development.On the short-term load forecasting of power system,a variant model of RNN-LSTM is tested in this paper.It effectively solves the problem of gradient explosion and disappearance caused by large amount of data input in classical RNN.On the basis of this model,optimization experiments are carried out under different super parameters to achieve better prediction results.The experimental results show that the accuracy of test set reaches 99.8%,which proves that the method proposed in this paper has certain reference value. | Jian LI Yehui PENG Ziyi CHENG Yuwei LI Jiapeng FAN Junpeng CHEN | 2022 | Meteorological and Environmental Research2022，13，4： | 1 |
| 3 | Synthesis and Characterization of Poly(terephthalic acid-2,5-furandicarboxylic acid-1,8-octanediol) Copolyester显示文摘Terephthalic acid,2,5-furandicarboxylic acid and 1,8-octanediol were adopted as monomers and antimony trioxide as catalyst,and poly(terephthalic acid-2,5-furandicarboxylic acid-1,8-octanediol) copolyesters identified as PEOT-x,where x is the mole fraction of furandicarboxylic acid in the samples,were synthesized by direct esterification.The molecular structure of the copolyesters was characterized by FTIR and 1H NMR spectroscopy.Gel permeation chromatography (GPC),differential scanning calorimetry (DSC),and thermogravimetric analysis (TGA) were used to characterize the molecular weight,molar mass dispersity,glass transition temperature,and thermal stability of the copolyesters,respectively.The mechanical properties of the samples were also investigated.The number-average molecular weight (M_(n)) of the samples varies from 9 700-18 800 g/mol,and molar mass dispersity (■=M_(w)/M_(n)) from 2.15-3.34.The initial decomposition temperature of the copolyesters is in the 332-356 ℃ range,with maximum decomposition rates at 390-410 ℃,while the glass transition temperature (Tg) varies from 0-33 ℃.Mechanical test shows that PEOT-10 has the highest tensile strength,while PEOT-90 has the largest tensile modulus and elongation at break.The experimental results show that these copolyesters can be synthesized with relatively high molecular weights,good thermal stability,and fair mechanical properties,which makes them excellent replacements for commercial polyesters,such as PET,and these properties can be tuned through the relative amounts of biomass monomer 2,5-furandicarboxylic acid used in the reactions. | 程正载 CHENG Junpeng CHEN Jun XIONG Jing SUN Xin JIA Ruyan YUAN Beibei Gauthier Mario | 2021 | Journal of Wuhan University of Technology(Materials Science)2021，36，4： | 1 |
| 4 | Effects of SnO on Structure and Propertiesof Borosilicate Glasses显示文摘Tin was found in the bottom of float borosilicate glasses.To simulate the enriched amounts of SnO found on the surface of the float borosilicate glasses,a series of glasses were produced in which the stannous concentration was varied from 0.1 wt% to 9.0 wt%,while the relative concentration of other components were held constant.Infrared spectra were obtained to probe the effect of increased amounts of SnO on the structure of the glass samples.The results show that SnO plays the role of an intermediate in glasses studied.When FO/SnO>1.0,SnO takes the role of network-former.And when FO/SnO<1.0,SnO can give the free oxygen as network-modifier.Besides,SnO has intensive effect on thermal performance of borosilicate glasses. | LU Ping CHENG Jinshu WAN Junpeng | 2008 | Journal of Wuhan University of Technology(Materials Science)2008，23，4： | 0 |
| 5 | Synthesis and Characterization of Novel Hydrolytically Degradable Polyesters显示文摘Six novel hydrolytically degradable polyesters were synthesized from thiodipropionic acid(TDPA)and five diols by melt polycondensation,and characterized by FT-IR,1H NMR,gel permeation chromatography,differential scanning calorimetry and thermogravimetry analysis.The polystyrene-equivalent number-average(Mn)and weight-average molecular weight(Mw)of these polyesters ranged from 4900-11100 Da and 7900-20879 Da,respectively,with PDI values of 1.48-1.98.The melting point varied from 62.3-127.9℃,and the 50%mass-loss temperature ranged between 387-417℃.The degradation of these polyesters was studied in terms of relative weight loss in distilled water at different pH.Weight losses of 14%-26%were obtained at pH 7.0,26%-38%at pH 6.0,and 32%-43%at pH 8.3 over a 20-week period.The ecotoxicity study suggested that safety of the synthesized polyesters for the eisenia foetida.These results indicate that these polyesters have a combination of good thermal and degradability behaviors,which can be tailored through selection of the diol monomers used in the synthesis. | SUN Xin CHENG Zhengzai DJOUONKEP Lesly Dasilva Wandji LAN Chupeng JIA Ruyan ZENG Sheng CHENG Junpeng TANG Ran LI Yi YUAN Beibei GAUTHLLER Mario | 2023 | Journal of Wuhan University of Technology(Materials Science)2023，38，2： | 0 |
| 6 | Exploiting ultra-large linear elasticity over a wide temperature range in nanocrystalline NiTi alloy显示文摘Many shape memory alloys can support large recoverable strains of a few percent by reversible stressinduced martensite transformation,yet they behave non-linear within a narrow operating temperature ra nge.Developing the bulk metallic materials with ultra-large linear elasticity over a wide tempe rature range has proven to be difficult.In this work,a material design concept was proposed,that is true elastic deformation and reversible twinning-detwinning deformation run in parallel to overcome this challe nge.By engineering the residual internal stress to realize the concurrency of true elastic deformation and twinning-detwinning deformation,a bulk nanocrystalline NiTi that possesses an ultra-large linear elastic strain up to 5.1 % and a high yield stress of 2.16 GPa over a wide temperature range of 270℃ was developed.This study offers a new avenue for developing the metallic materials with ultra-large linear elasticity over a wide temperature range of 270℃(from 70℃ to-197℃). | Zhen Sun Shijie Hao Genfa Kang Yang Ren Junpeng Liu Ying Yang Xiangguang Kong Bo Feng Cheng Wang Kun Zhao Lishan Cui | 2020 | Journal of Materials Science & Technology2020，54，22： | 0 |
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