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| 1 | Echinacoside Protects Against MPP+-Induced Neuronal Apoptosis via ROS/ATF3/CHOP Pathway Regulation显示文摘Echinacoside(ECH) is protective in a mouse model of Parkinson's disease(PD) induced by 1-methyl-4-phenylpyridinium ion(MPP^+). To investigate the mechanisms involved, SH-SY5Y neuroblastoma cells were treated with MPP^+or a combination of MPP^+and ECH,and the expression of ATF3(activating transcription factor3), CHOP(C/EBP-homologous protein), SCNA(synuclein alpha), and GDNF(glial cell line-derived neurotrophic factor) was assessed. The results showed that ECH significantly improved cell survival by inhibiting the generation of MPP^+-induced reactive oxygen species(ROS). In addition, ECH suppressed the ROS and MPP^+-induced expression of apoptotic genes(ATF3, CHOP, and SCNA). ECH markedly decreased the MPP^+-induced caspase-3 activity in a dose-dependent manner. ATF3-knockdown also decreased the CHOP and cleaved caspase-3 levels and inhibited the apoptosis induced by MPP^+.Interestingly, ECH partially restored the GDNF expression that was down-regulated by MPP^+. ECH also improved dopaminergic neuron survival during MPP^+treatment and protected these neurons against the apoptosis induced by MPTP. Taken together, these data suggest that the ROS/ATF3/CHOP pathway plays a critical role in mechanisms by which ECH protects against MPP^+-induced apoptosis in PD. | Qing Zhao Xiaoyan Yang Dingfang Cai Ling Ye Yuqing Hou Lijun Zhang Jiwei Cheng Yuan Shen Kaizhe Wang Yu Bai | 2016 | Neuroscience Bulletin2016，32，4： | 14 |
| 2 | A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China. | Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu | 2006 | Cellular & Molecular Immunology2006，3，6： | 9 |
| 3 | Review on the NO removal from flue gas by oxidation methods显示文摘Due to the increasingly strict emission standards of NOx on various industries,many traditional flue gas treatment methods have been gradually improved.Except for selective catalytic reduction(SCR)and selective non-catalytic reduction(SNCR)methods to remove NOx from flue gas,theoxidation method is paying more attention to NOx removal now because of the potential to simultaneously remove multiple pollutants from flue gas.This paper summarizes the efficiency,reaction conditions,effect factors,and reaction mechanism of NO oxidation from the aspects of liquid-phase oxidation,gas-phase oxidation,plasma technology,and catalytic oxidation.The effects of free radicals and active components of catalysts on NO oxidation and the combination of various oxidation methods are discussed in detail.The advantages and disadvantages of different oxidation methods are summarized,and the suggestions for future research on NO oxidation are put forward at the end.The review on the NO removal by oxidation methods can provide new ideas for future studies on the NO removal from flue gas. | Meng Si Boxiong Shen George Adwek Lifu Xiong Lijun Liu Peng Yuan Hongpei Gao Cai Liang Qihai Guo | 2021 | Journal of Environmental Sciences2021，33，3： | 8 |
| 4 | Chinese herb microneedle patch for wound healing显示文摘Traditional Chinese medicine and Chinese herbs have a demonstrated value for disease therapy and sub-health improvement.Attempts in this area tend to develop new forms to make their applications more convenient and wider.Here,we propose a novel Chinese herb microneedle(CHMN)patch by integrating the herbal extracts,Premna microphylla and Centella asiatica,with microstructure of microneedle for wound healing.Such path is composed of sap extracted from the herbal leaves via traditional kneading method and solidified by plant ash derived from the brine induced process of tofu in a well-designed mold.Because the leaves of the Premna microphylla are rich in pectin and various amino acids,the CHMN could be imparted with medicinal efficacy of heat clearing,detoxicating,detumescence and hemostatic.Besides,with the excellent pharmaceutical activity of Asiatic acid extracted from Centella asiatica,the CHMN is potential in promoting relevant growth factor genes expression in fibroblasts and showing excellent performance in anti-oxidant,anti-inflammatory and anti-bacterial activity.Taking advantages of these pure herbal compositions,we have demonstrated that the derived CHMN was with dramatical achievement in anti-bacteria,inhibiting inflammatory,collagen deposition,angiogenesis and tissue reconstruction during the wound closure.These results indicate that the integration of traditional Chinese herbs with progressive technologies will facilitate the development and promotion of traditional Chinese medicine in modern society. | Junjie Chi Lingyu Sun Lijun Cai Lu Fan Changmin Shao Luoran Shang Yuanjin Zhao | 2021 | Bioactive Materials2021，6，10： | 8 |
| 5 | Stomatocyte structural color-barcode micromotors for multiplex assays显示文摘Artificial micromotors have a demonstrated value in the biomedical area.Attempts to develop this technology tend to impart micromotors with novel functions to improve the values.Herein,we present novel structural color-barcode micromotors for the multiplex assays.We found that,by rapidly extracting solvent and assembling monodispersed nanoparticles in droplets,it could form stomatocyte colloidal crystal clusters,which not only showed striking structural colors and characteristic reflection peaks due to their ordered nanoparticles arrangement,but also provided effective cavities for the integration of functional elements.Thus,the micromotors with catalysts or magnetic elements in their cavities,as well as with the corresponding structural color coding,could be achieved by using the platinum and ferric oxide dispersed pre-gel to fill and duplicate the stomatocyte colloidal crystal clusters.We have demonstrated that the self-movement of these structural color-barcode micromotors could efficiently accelerate the mixing speed of the detection sample and greatly increase the probe-target interactions towards faster and more sensitive single or multiplex detection,and the magnetism of these barcode micromotors enables the flexible collection of the micromotors,which could facilitate the detection processes.These features make the stomatocyte structural color-barcode micromotors ideal for biomedical applications. | Lijun Cai Huan Wang Yunru Yu Feika Bian Yu Wang Keqing Shi Fangfu Ye Yuanjin Zhao | 2020 | National Science Review2020，7，3： | 7 |
| 6 | Identification of Pns12 as the second silencing suppressor of Rice gall dwarf virus显示文摘RNA silencing is a conserved mechanism found ubiquitously in eukaryotic organisms.It has been used to regulate gene expression and development.In addition,RNA silencing serves as an important mechanism in plants' defense against invasive nucleic acids,such as viruses,transposons,and transgenes.As a counter-defense,most plants,and some animal viruses,encode RNA silencing suppressors to interfere at one or several points of the silencing pathway.In this study,we showed that Pns12 of RGDV (Rice gall dwarf virus) exhibits silencing suppressor activity on the reporter green fluorescent protein in transgenic Nicotiana benthamiana line 16c.Pns12 of RGDV suppressed local silencing induced by sense RNA but had no effect on that induced by dsRNA.Expression of Pns12 also enhanced Potato virus X pathogenicity in N.benthamiana.Collectively,these results suggested that RGDV Pns12 functions as a virus suppressor of RNA silencing,which might target an upstream step of dsRNA formation in the RNA silencing pathway.Furthermore,we showed that Pns12 is localized mainly in the nucleus of N.benthamiana leaf cells. | WU JianGuo WANG ChunZheng DU ZhengGuo CAI LiJun HU MeiQun WU ZuJian LI Yi XIE LianHui | 2011 | Science China(Life Sciences)2011，54，3： | 7 |
| 7 | 雷公藤红素通过靶向核受体Nur77促进损伤线粒体自噬而抑制炎症反应显示文摘文章简介线粒体在细胞死亡、自噬、免疫和炎症中起着不可或缺的作用。前期研究发现,孤儿核受体Nur77通过靶向线粒体诱导细胞凋亡。本文报道了Nur77作为具有抗炎作用的雷公藤红素的直接靶点,介导雷公藤红素通过自噬清除损伤线粒体,抑制炎症反应而达到治疗炎症疾病包括肥胖症的功能。 | Mengjie Hu Qiang Luo Gulimiran Alitongbieke Shuyi Chong Chenting Xu Lei Xie Xiaohui Chen Duo Zhang Yuqi Zhou Zhaokai Wang Xiaohong Ye Lijun Cai Fang Zhang Huibin Chen Fuquan Jiang Hui Fang Shanjun Yang Jie Liu Maria T.Diaz-Meco Ying Su Hu Zhou Jorge Moscat Xiangzhi Lin 张晓坤 | 2018 | 科学新闻2018，0，4： | 3 |
| 8 | Biomolecule-assisted Solvothermal Synthesis of Bismuth Sulfide Nanorods显示文摘A simple biomolecule-assisted synthetic route has been successfully developed to prepare bismuth sulfide(Bi 2 S 3 ) nanorods under solvothermal conditions.In the synthetic system,pentahydrate bismuth nitrate was employed to supply Bi source and L-cystine was used as sulfide source and complexing agent.The morphology,structure,and phase composition of the as-prepared Bi 2 S 3 products were characterized by X-ray diffraction(XRD),energy dispersion spectroscopy(EDS),X-ray photoelectron spectroscopy(XPS),field-emission scanning electron microscopy(FESEM),transmission electron microscopy(TEM),selected area electron diffraction(SAED),and high-resolution transmission electron microscopy(HRTEM).The experimental results show that the nanorods have uniform diameter of 100-200 nm and length of 2-4 μm.The possible formation mechanism for the bismuth sulfide nanorods was discussed. | Jiasong Zhong Weidong Xian Lijun Liu Xinyu Yan Wen Cai Jingfeng Zhang Xiaojuan Liang | 2010 | Journal of Materials Science & Technology2010，26，5： | 3 |
| 9 | Identification and characterization of tomato gibberellin 2-oxidases (GA2oxs) and effects of fruit-specific SlGA2ox1 overexpression on fruit and seed growth and development显示文摘Gibberellins(GAs)play a crucial role in growth and development of the tomato fruit.Previously published studies focusing on the effect of GAs on tomato fruits used chemical treatments,constitutive overexpression or silencing of GA biosynthetic and catabolic genes globally throughout the plant.Fruit-specific overexpression of GA catabolic enzyme genes GA2-oxidases(GA2oxs),however,may provide an alternative method to study the role of endogenous GAs on the fruit development.In this study,we have identified 11 SlGA2ox proteins in tomato that are classified into three subgroups.Motif analysis and multiple sequence alignments have demonstrated that all SlGA2oxs,except SlGA2ox10,have similar motif compositions and high-sequence conservation.Quantitative reverse transcription-PCR analysis has showed that SlGA2oxs exhibit differential expression patterns in tomato fruits at different developmental stages.When the fruit-specific promoter TFM7 was used to control the expression of SlGA2ox1,we observed no changes in growth and development of vegetative organs.However,fruit weight,seed number and germination rate were significantly affected.We also treated tomato fruits with GA biosynthesis inhibitor and observed phenotypes similar to those of the transgenic fruits.Furthermore,we have demonstrated that expression of cell expansion and GA responsive genes were downregulated in transgenic tomato fruits,supporting that overexpression of the SlGA2ox1 leads to reduction in endogenous GAs.This study provides additional evidence that endogenous GAs and the SlGA2ox1 gene play an important role in controlling on fruit weight,seed development and germination in tomato plant. | Shen Chen Xiaojing Wang Liying Zhang Shanshan Lin Decai Liu Quanzhi Wang Shanya Cai Rania El-Tanbouly Lijun Gan Han Wu Yi Li | 2016 | Horticulture Research2016，3，1： | 3 |
| 10 | Recent progress in Chinese fusion research based on superconducting tokamak configuration显示文摘Fusion energy is a promising source of clean energy,which could solve energy shortages and environmental pollution.Research into controlled fusion energy has been ongoing for over half a century.China has created a clear roadmap for magnetic confinement fusion development,where superconducting tokamaks will be used in commercial fusion reactors.The Experimental Advanced Superconducting Tokamak(EAST)is the world’s first fully superconducting tokamak with upper and lower divertors,which aims at longpulse,steady-state,H-mode operation,and 101-s H-mode discharge had been achieved.In 2007,China joined the International Thermonuclear Experimental Reactor(ITER)and became one of its seven members.Thirteen procurement packages are undertaken by China,covering superconducting magnets,power supplies,plasma-facing components(PFCs),diagnostics,etc.To bridge the gap between the ITER and fusion demonstration power plants(DEMOs),China is planning to build the Chinese Fusion Engineering Testing Reactor(CFETR)to demonstrate related technologies and physics models.The engineering design of the CFETR was completed in 2020,and Comprehensive Research Facilities for Fusion Technology(CRAFT)are being constructed to explore the key technologies used in the CFETR. | Jinxing Zheng Jinggang Qin Kun Lu Min Xu Xuru Duan Guosheng Xu Jiansheng Hu Xianzu Gong Qing Zang Zhihong Liu Liang Wang Rui Ding Jiming Chen Pengyuan Li Lei Xue Lijun Cai Yuntao Song | 2022 | The Innovation2022，3，4： | 3 |
| 11 | Optimal replacement policy of products with repair-cost threshold after the extended warranty显示文摘The reliability of the product sold under a warranty is usually maintained by the manufacturer during the warranty period.After the expiry of the warranty, however, the consumer confronts a problem about how to maintain the reliability of the product.This paper proposes, from the consumer's perspective, a replacement policy after the extended warranty, under the assumption that the product is sold under the renewable free replacement warranty(RFRW) policy in which the replacement is dependent on the repair-cost threshold. The proposed replacement policy is the replacement after the extended warranty is performed by the consumer based on the repair-cost threshold or preventive replacement(PR) age, which are decision variables. The expected cost rate model is derived from the consumer's perspective. The existence and uniqueness of the optimal solution that minimizes the expected cost rate per unit time are offered. Finally, a numerical example is presented to exemplify the proposed model. | Lijun Shang Zhiqiang Cai | 2017 | Journal of Systems Engineering and Electronics2017，28，4： | 2 |
| 12 | Fast Community Detection Based on Distance Dynamics显示文摘The distance dynamics model is excellent tool for uncovering the community structure of a complex network. However, one issue that must be addressed by this model is its very long computation time in large-scale networks. To identify the community structure of a large-scale network with high speed and high quality, in this paper, we propose a fast community detection algorithm, the F-Attractor, which is based on the distance dynamics model. The main contributions of the F-Attractor are as follows. First, we propose the use of two prejudgment rules from two different perspectives: node and edge. Based on these two rules, we develop a strategy of internal edge prejudgment for predicting the internal edges of the network. Internal edge prejudgment can reduce the number of edges and their neighbors that participate in the distance dynamics model. Second, we introduce a triangle distance to further enhance the speed of the interaction process in the distance dynamics model. This triangle distance uses two known distances to measure a third distance without any extra computation. We combine the above techniques to improve the distance dynamics model and then describe the community detection process of the F-Attractor. The results of an extensive series of experiments demonstrate that the F-Attractor offers high-speed community detection and high partition quality. | Lei Chen Jing Zhang Lijun Cai Ziyun Deng | 2017 | Tsinghua Science and Technology2017，22，6： | 2 |
| 13 | The CRL3^(BTBD9) E3 ubiquitin ligase complex targets TNFAIP1 for degradation to suppress cancer cell migration显示文摘Tumor necrosis factor alpha-induced protein 1(TNFAIP1)modulates a plethora of important biological processes,including tumorigenesis and cancer cell migration.However,the regulatory mechanism of TNFAIP1 degradation remains largely elusive.In the present study,with a label-free quantitative proteomic approach,TNFAIP1 was identified as a novel ubiquitin target of the Cullin-RING E3 ubiquitin ligase(CRL)complex.More importantly,Cul3-ROC1(CRL3),a subfamily of CRLs,was identified to specifically interact with TNFAIP1 and promote its polyubiquitination and degradation.Mechanistically,BTBD9,a specific adaptor component of CRL3 complex,was further defined to bind and promote the ubiquitination and degradation of TNFAIP1 in cells.As such,downregulation of BTBD9 promoted lung cancer cell migration by upregulating the expression of TNFAIP1,whereas TNFAIP1 deletion abrogated this effect.Finally,bioinformatics and clinical sample analyses revealed that BTBD9 was downregulated while TNFAIP1 was overexpressed in human lung cancer,which was associated with poor overall survival of patients.Taken together,these findings reveal a previously unrecognized mechanism by which the CRL3^(BTBD9) ubiquitin ligase controls TNFAIP1 degradation to regulate cancer cell migration. | Lihui Li Wenjuan Zhang Yue Liu Xiaojun Liu Lili Cai Jihui Kang Yunjing Zhang Wenlian Chen Changsheng Dong Yanmei Zhang Mingsong Wang Wenyi Wei Lijun Jia | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 2 |
| 14 | Designing bioactive micro-/nanomotors for engineered regeneration显示文摘Micro/nanoscale motors(MNMs)have been regarded as promising tools in the field of engineered regeneration due to unique property of autonomous motion.Herein,a review on the advancements of MNMs in the area of engineered regeneration is presented,covering aspects from their propulsion mechanisms to their frontiers in engineered regeneration,listing the revolutionary applications in biosensing,medical imaging,drug delivery and tissue engineering.Finally,challenges and future directions of MNMs are finally discussed on the basis of the achievements. | Lijun Cai Dongyu Xu Hanxu Chen Li Wang Yuanjin Zhao | 2021 | Engineered Regeneration2021，2，1： | 2 |
| 15 | Boston Ivy-Inspired Disc-Like Adhesive Microparticles for Drug Delivery显示文摘Microparticles with strong adherence are expected as efficient drug delivery vehicles.Herein,we presented an ingenious hydrogel microparticle recapitulating the adhesion mechanism of Boston ivy tendrils adhesive discs(AD)for durable drug delivery.The particles were achieved by replicating a silica colloidal crystal aggregates assembled in a droplet template after rapid solvent extraction.Due to their unique shape,the nanostructure,and the sticky hydrogel component,such novel microparticles exhibited prominent adhesive property to the wet tissue environment.It was demonstrated that the bioinspired microcarriers loading with dexamethasone had a good therapeutic effect for ulcerative colitis due to the strong adhesion ability for prolonging the maintenance of drug availability.These virtues make the biomimetic microparticles potentially ideal for many practical clinical applications,such as drug delivery,bioimaging,and biodiagnostics. | Lijun Cai Guopu Chen Yuetong Wang Cheng Zhao Luoran Shang Yuanjin Zhao | 2021 | Research2021，，1： | 2 |
| 16 | Osteosarcoma metastasis: prospective role of ezrin显示文摘 | Yuanjin Zhang Ling Zhang Guofu Zhang Songbai Li Jun Duan Jie Cheng Guozhen Ding Chibing Zhou Jie Zhang Pengcheng Luo Dongbing Cai Lianghong Kuang Yichun Zhou Liqun Tong Xiangdong Yu Lifang Zhang Lijun Xu Li Yu Xiaomei Shi Aihong Ke | 2014 | Tumor Biology2014，，6： | 1 |
| 17 | Simultaneous coordinated tuning of PSS and FACTS damping controllers in large power sys- tems显示文摘 | Cai Lijun Erlich Istvan | 2005 | IEEE Trans on Power Systems2005，20，1： | 1 |
| 18 | Study on activated carbon derived from sewage sludge for adsorption of gaseous formaldehyde显示文摘 | Qingbo Wen Caiting Li Zhihong Cai Wei Zhang Hongliang Gao Lijun Chen Guangming Zeng Xin Shu Yapei Zhao | 2010 | Bioresource Technology2010，，2： | 1 |
| 19 | An analytical model for spontaneous imbibition in fractal porous media including gravity显示文摘 | Jianchao Cai Xiangyun Hu Dag Chun Standnes Lijun You | 2012 | Colloids and Surfaces A: Physicochemical and Engineering Aspects2012，，： | 1 |
| 20 | Detecting rice stripe virus (RSV) in the small brown planthopper (Laodelphax striatellus) withhigh specificity by RT-PCR显示文摘 | Cai Lijun Ma Xizhi Kang Lin | 2003 | Journal of Virological Methods2003，112，： | 1 |
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