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| 1 | Effect of ultrasonic micro-forging treatment on microstructure and mechanical properties of GH3039 superalloy processed by directed energy deposition显示文摘In this work,ultrasonic micro-forging treatment(UMFT)was introduced to achieve homogeneous microstructure,reduce defects and improve mechanical properties of GH3039 superalloy cladding layer processed by directed energy deposition(DED).The microstructure,defects and mechanical properties of the cladding layers treated by UMFT with different ultrasonic powers(UIPs)were investigated.Results revealed a gradient structure as equiaxed grains distributed at the top,a columnar-to-equiaxed transition(CET)region that mixed of columnar dendrites and equiaxed grains distributed at the middle and columnar dendrites at the bottom of the cladding layer was formed.After UMFT,the proportion of equiaxed grains was increased,the average size of equiaxed grains was refined to 10μm from 16μm,the orientation of grains was more uniform and the phases enriched of Al,Ti,C,Nb and Mo were precipitated.The grain refinement can be attributed to the fracture of columnar dendrites induced by the ultrasonic vibration during solidification.Besides,the porosity of the cladding layer was reduced after UMFT.The microhardness of the cladding layers exhibited a depth-dependent gradient at the top region.The microhardness of the top surface was the highest and showed an increasing trend with the increase of UIP.The microhardness of different grain morphologies exhibited no substantial difference.However,due to grain refinement and precipitation of strengthening phase induced by UMFT,the microhadness of some local locations were improved.These results indicated UMFT has a significant effect on improving the microstructure,defects and mechanical properties of the deposited cladding layer. | Qingqing Li Yong Zhang Jie Chen Bugao Guo Weicheng Wang Yuhai Jing Yong Liu | 2021 | Journal of Materials Science & Technology2021，，11： | 4 |
| 2 | A real-time 3D scanning system for pavement distortion inspection显示文摘 | Li Qingguang Yao Ming Yao Xun Xu Bugao | 2010 | Measurement Science and Technology2010，21，1： | 1 |
| 3 | Snippet counting for cotton length distribution measurement using image analysis显示文摘 | XU Weilin XU Bugao LI Wenbin | 2008 | Textile Research Journal2008，78，4： | 1 |
| 4 | Separation of clustered fibers in cross-sectional images using image set theory显示文摘 | WAN Yah YAO Li XU Bugao | 2009 | Textile Research Journal2009，79，18： | 1 |
| 5 | Automatic segmentation of fiber cross sections by dual thresholding 显示文摘 | Wan Yan Yao Li Xu Bugao | 2012 | J Eng Fiber Fabr2012，7，1： | 1 |
| 6 | Snippet counting for cotton length distribution measurement using image analysis 显示文摘 | Xu Weilin Xu Bugao LI Wenbin CUI Weigan | 2008 | Textile Research Journal2008，78，： | 1 |
| 7 | Mining Unknown Porcine Protein Isoforms by Tissue-based Map of Proteome Enhances Pig Genome Annotation显示文摘A lack of the complete pig proteome has left a gap in our knowledge of the pig genome and has restricted the feasibility of using pigs as a biomedical model.In this study,we developed a tissue-based proteome map using 34 major normal pig tissues.A total of 5841 unknown protein isoforms were identified and systematically characterized,including 2225 novel protein isoforms,669 protein isoforms from 460 genes symbolized beginning with LOC,and 2947 protein isoforms without clear NCBI annotation in the current pig reference genome.These newly identified protein isoforms were functionally annotated through profiling the pig transcriptome with high-throughput RNA sequencing of the same pig tissues,further improving the genome annotation of the corresponding protein-coding genes.Combining the well-annotated genes that have parallel expression pattern and subcellular witness,we predicted the tissue-related subcellular locations and potential functions for these unknown proteins.Finally,we mined 3081 orthologous genes for 52.7% of unknown protein isoforms across multiple species,referring to 68 KEGG pathways as well as 23 disease signaling pathways.These findings provide valuable insights and a rich resource for enhancing studies of pig genomics and biology,as well as biomedical model application to human medicine. | Pengju Zhao Xianrui Zheng Ying Yu Zhuocheng Hou Chenguang Diao Haifei Wang Huimin Kang Chao Ning Junhui Li Wen Feng Wen Wang George ELiu Bugao Li Jacqueline Smith Yangzom Chamba Jian-Feng Liu | 2021 | Genomics, Proteomics & Bioinformatics2021，19，5： | 0 |
| 8 | Cloning, Sequence Analysis, and Prokaryotic Expression of the Porcine DECR1 Gene显示文摘2,4-dienoyl-CoA reductase 1 (DECR1) is the key rate-limiting enzyme in the metabolism of polyunsaturated fatty acids. Although this protein has been studied in a variety of mammals, its role in porcine is yet to be fully elucidated. However, it is a candidate determinant/indicator of meat quality, growth traits, and carcass quality. Here, we employed RT-PCR and rapid amplification of cDNA ends (RACE) analysis to amplify the full-length cDNA of DECR1 from Mashen pig liver, and cloned it into the expression vector pET-32a+. After confirmation by sequencing and restriction analysis, the recombinant plasmid was transformed into E. coli BL21 cells. The cDNA of pig DECR1 contained 2,352 nucleotides, including a 987 bp open reading frame flanked by a 53 bp 5′-untranslated region (UTR) and a 1,312 bp 3′-UTR. The pig DECR1 coding sequence encoded 328 amino acid residues, which shared 99%, 88%, 87%, 87%, 87%, 87%, and 83% identity with those of Sus scrofa (predicted), Bos taurus, Homo sapiens, Macaca mulatta, Pan troglodytes, Equus caballus, Canis, and Mus musculus, respectively. SDS-PAGE analysis revealed that the recombinant protein was expressed and that the expression level reached its highest level after 4 h induction. Western blot analysis indicated that the molecular weight of the expressed protein was the same as that predicted, approximately 35 kDa. Collectively these data provide the basis for further studies into the physiological functions and molecular mechanisms of the pig DECR1 gene. | Bugao Li Xiaohong Guo Guoqing Cao Xiaofen Yang Xiaojing Wang Zhongxiao Zhou | 2011 | Journal of Animal Science and Biotechnology2011，2，2： | 0 |
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