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| 1 | Early dynamic transcriptomic changes during preoperative radiotherapy in patients with rectal cancer: A feasibility study显示文摘AIM: To develop novel biomarkers of rectal radiotherapy, we measured gene expression profiles on biopsies taken before and during preoperative radiotherapy. METHODS: Six patients presenting with a locally advanced rectal cancer (T>T2, N0/Nx, M0) eligible for preoperative radiotherapy (45 Gy in 25 fractions) were selected in a pilot study. Six tumor and 3 normal tissues biopsies were taken before and during radiotherapy,after a dose of 7.2 Gy at a median time of 1 h following irradiation (0:27-2:12). Tumor or normal tissue purity was assessed by a pathologist prior to RNA extraction. Mean RNA content was 23 μg/biopsy (14-37) before radiotherapy and 22.7 μg/biopsy (12-35) during radiotherapy. After RNA amplification, biopsies were analysed with 54K HG-U133A Plus 2.0 Affymetrix expression micro-arrays. Data were normalized according to MAS5 algorithm. A gene expression ratio was calculated as: (gene expression during radiotherapy-gene expression before radiotherapy)/gene expression before radiotherapy. Were selected genes that showed a ratio higher than ± 0.5 in all 6 patients. RESULTS: Microarray analysis showed that preoperative radiotherapy significantly up-regulated 31 genes and down-regulated 6 genes. According to the Gene Ontology project classification, these genes are involved in protein metabolism (ADAMDEC1 ; AKAP7 ; CAPN5 ; CLIC5 ; CPE ; CREB3L1 ; NEDD4L ; RAB27A), ion transport (AKAP7 ; ATP2A3 ; CCL28 ; CLIC5 ; F2RL2 ; NEDD4L ; SLC6A8), transcription (AKAP7 ; CREB3L1 ; ISX ; PAB-PC1L ; TXNIP), signal transduction (CAPN5 ; F2RL2 ; RA- B27A ; TNFRSF11A), cell adhesion (ADAMDEC1 ; PXDN ; SPON1 ; S100A2), immune response (CCL28 ; PXDN ; TNFRSF11A) and apoptosis (ITM2C ; PDCD4 ; PVT1). Up-regulation of 3 genes (CCL28 ; CLIC5 ; PDCD4) was detected by 2 different probes and up-regulation of 2 genes (RAB27A ; TXNIP) by 3 probes. CONCLUSION: Micro-arrays can efficiently assess early transcriptomic changes during preoperative radiotherapy for rectal cancer, and may help better understand tumor radioresistance. | Stephane Supiot Wilfried Gouraud Loc Campion Pascal Jezéquel Bruno Buecher Josiane Charrier Marie-Francoise Heymann Marc-Andre Mahé Emmanuel Rio Michel Chérel | 2013 | World Journal of Gastroenterology2013，19，21： | 2 |
| 2 | Vague sets显示文摘 | Gau W Buecher D J | 1993 | IEEE Transaction on Systems Man and Cybemetics1993，23，2： | 1 |
| 3 | A routine ESE-like state facilitates transgenesis and homologous recombination in hu- man pluripotent stem cells 显示文摘 | BUECHER C CHEN H POLO M | 2010 | Cell Stem Cell2010，6，6： | 1 |
| 4 | Site dependence of the energy collection of PV modules显示文摘 | BUECHER K | 1997 | Solar Energy Materials and Solar Cells1997，47，： | 1 |
| 5 | Molecular a- nalysis of the effect of short-chain fatty acids on intestinal cell proliferation显示文摘 | Blottire H M Buecher B Galmiche J P | 2003 | The Proceedings of the Nutrition Society2003，62，1： | 1 |
| 6 | Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation显示文摘 | Blottiere HM Buecher B Galmiche JP | 2003 | Proc Nutr Soc2003，62，1： | 1 |
| 7 | Role of microsat- ellite instability in the management of colorectal cancers 显示文摘 | Buecher B Cacheux W Rouleau E | 2013 | Dig Liver Dis2013，45，6： | 1 |
| 8 | Growth inhibitory effect of celecoxib and rofecoxib on human colorectal carcinoma cell lines显示文摘 | Buecher B Bouancheau D Broquet A | 2005 | AntiCancor Res2005，25，1： | 1 |
| 9 | Role of microsatellite instability in the management of colorectal cancers显示文摘 | Bruno Buecher Wulfran Cacheux Etienne Rouleau Barbara Dieumegard Emmanuel Mitry Astrid Lièvre | 2013 | Digestive and Liver Disease2013，，6： | 1 |
| 10 | Use of a multiplex PCR/RT-PCR approach to assess the viral causes of influenza-like illnesses in Cambodia during three consecutive dry seasons显示文摘 | Buecher C Mardy S Wang W | 2010 | J Med Virol2010，82，10： | 1 |
| 11 | Site dependence of the energy collection of PV modules显示文摘 | BUECHER K | 1997 | Solar Energy Materials and Solar Cells1997，47，： | 1 |
| 12 | Photovoltaic modules in buildings:performance and safety显示文摘 | BUECHER K | 1998 | Renewable Energy1998，15，14： | 1 |
| 13 | The effect ofedge permeability and habitat geometry on emigration frompatches of habitat显示文摘 | STAMPS J A BUECHER M KRISHNAN V V | 1987 | Amer Nat1987，129，： | 1 |
| 14 | Growth inhibitory effect of celecoxib and rofecoxib on human colorectal carcinoma cell lines 显示文摘 | Buecher B Bouancheau D Broquet A | 2005 | Anti Cancer Res2005，25，1： | 1 |
| 15 | Appearance of Chelophyes appendiculata and Abylopsis tetragona (Cnidaria, Siphonophora) in the Bay of Villefranche, northwestern Mediterranean显示文摘 | Buecher E | 1999 | Journal of Sea Research1999，41，： | 1 |
| 16 | Colorectal Adenomatous Polyposis Associated with MYH Mutations: Genotype and Phenotype Characteristics显示文摘 | Guillaume Bouguen M.S. Sylvain Manfredi M.D. Martine Blayau M.D. Catherine Dugast M.D. Bruno Buecher M.D. Dominique Bonneau Ph.D. Laurent Siproudhis Ph.D. Véronique David Ph.D. Jean-Fran?ois Bretagne Ph.D | 2007 | Diseases of the Colon & Rectum2007，，10： | 1 |
| 17 | Papular elastorrhexis,report of five cases显示文摘 | Buecher SA Itin P | 2002 | Dermatology2002，205，2： | 1 |
| 18 | Vague Sets显示文摘 | Buecher D J | 1993 | IEEE Transaction on Systems Man and Cybernetics1993，23，2： | 1 |
| 19 | Combinations of cytochrome P450 gene polymorphisms enhancing the risk for sporadic colorectal cancer related to red meat consumption显示文摘 | Küry S Buecher B Robiou-du-Pont S | | 0，，07： | 1 |
| 20 | Growth inhibi- tory effect of celecoxib and rofecoxib on human colorectal carcinoma cell lines 显示文摘 | Buecher B Bouancheau D Broquet A | 2005 | Anticancer Res2005，25，1： | 1 |
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