|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Effects of insulin and pathway inhibitors on the PI3K-Akt- mTOR phosphorylation profile in acute myeloid leukemia cells显示文摘The phosphatidylinositol 3-kinase(PI3K)-Akt-mechanistic target of rapamycin(mTOR)pathway is constitutively activated in human acute myeloid leukemia(AML)cells and is regarded as a possible therapeutic target.Insulin is an agonist of this pathway and a growth factor for AML cells.We characterized the effect of insulin on the phosphorylation of 10 mediators in the main track of the PI3K-Akt-mTOR pathway in AML cells from 76 consecutive patients.The overall results showed that insulin significantly increased the phosphorylation of all investigated mediators.However,insulin effects on the pathway activation profile varied among patients,and increased phosphorylation in all mediators was observed only in a minority of patients;in other patients,insulin had divergent effects.Global gene expression profiling and proteomic/phosphoproteomic comparisons suggested that AML cells from these two patient subsets differed with regard to AML cell differentiation,transcriptional regulation,RNA metabolism,and cellular metabolism.Strong insulin-induced phosphorylation was associated with weakened antiproliferative effects of metabolic inhibitors.PI3K,Akt,and mTOR inhibitors also caused divergent effects on the overall pathway phosphorylation profile in the presence of insulin,although PI3K and Akt inhibition caused a general reduction in Akt pT308 and 4EBP1 pT36/pT45 phosphorylation.For Akt inhibition,the phosphorylation of upstream mediators was generally increased or unaltered.In contrast,mTOR inhibition reduced mTOR pS2448 and S6 pS244 phosphorylation but increased Akt pT308 phosphorylation.In conclusion,the effects of both insulin and PI3K-Akt-mTOR inhibitors differ between AML patient subsets,and differences in insulin responsiveness are associated with differential susceptibility to metabolic targeting. | Ina Nepstad Kimberley Joanne Hatfield Ida Sofie Grønningsæter Elise Aasebø Maria Hernandez-Valladares Karen Marie Hagen Kristin Paulsen Rye Frode SBerven Frode Selheim Håkon Reikvam Øystein Bruserud | 2019 | Signal Transduction and Targeted Therapy2019，4，1： | 4 |
| 2 | Expression and release of adhesion molecules by human acute myelogenous leukemia blasts 显示文摘 | Bruserud O Ulvestad E | 1999 | Leuk Res1999，23，2： | 1 |
| 3 | Expression and release of adhesion molecules by human acute myelogenous leukemia blasts显示文摘 | Bruserud Φ Ulvestad E | 1999 | Leukemia Research1999，23，： | 1 |
| 4 | Clonogenic acute myelogenous leukemia cells are heterogeneous with regard to regulation of differentiation and effect of epigenetic pharmacological targeting显示文摘 | Ryningen A Stapnes C Bruserud | 2007 | Leuk Res2007，31，： | 1 |
| 5 | Leptin in human acute myelogenous leukemia:studies of in vivo levels and in vitro effects on native functional leukemia blasts显示文摘 | Bruserud O Huang TS Glenjen N | 2002 | Haematologica2002，87，6： | 1 |
| 6 | Flow cytometric measurement of apoptosis and necrosis in cryopreserved PBPC concentrates from patientswith malignant diseases显示文摘 | Abrahamsen JF Bakken AM Bruserud O | 2002 | Bone Marrow Transplant2002，29，2： | 1 |
| 7 | Interleukin 4 responses in acute leukaemia patients with severe chemotherapy induced leucopenia显示文摘 | Bruserud O Ulvestad E Halstensen A | 1997 | Eur J Haematol1997，59，： | 1 |
| 8 | Cryopreserving human peripheral blood progenitor cells with 5-percent rather than 10-percent DMSO results in less apoptosis and necrosis in CD34 + cells显示文摘 | Abranhamsen J F Bakken A M Bruserud Φ | 2002 | Transfusion2002，42，12： | 1 |
| 9 | Ther- apeutic efficacy of platelet transfusion in patients with a- cute leukemia:an evaluation of methods 显示文摘 | Apelseth TO Bruserud O Wentzel-Larsen T | 2010 | Transfusion2010，50，4： | 1 |
| 10 | Expression and release of adhesion molecules by human acute myelogenous leukemia blasts显示文摘 | Bruserud O Ulvestad E | 1999 | Leuk Res1999，23，2： | 1 |
| 11 | Clonogenic acute myeloge-nous leukemia cells are heterogeneous with regard to regulationof different-iation and effect of epigenetic pharmacological target-ing显示文摘 | Ryningen A Stapnes C Bruserud 0 | 2007 | Leuk Res2007，31，9： | 1 |
| 12 | Expression and release of adhesion molecules by human acute myelogenous leukemia blasts 显示文摘 | Bruserud O Ulvestad E | 1999 | Leuk- Res1999，23，： | 1 |
| 13 | A management proogram for Primary thrombocytopenia显示文摘 | Kuntsen H Bruserud O | | Tidsskr Nor Laegeforen0，119，： | 1 |
| 14 | Acute promyelocytic leukaemia显示文摘 | Reikvam H Hovland R Bruserud O | 2014 | Tidsskr Nor Laegeforen2014，134，10： | 1 |
| 15 | Lipoteichoic acid derived from Enterococcus faecalis modulates the functional characteristics of both normal peripheral blood leukocytes and native human acute myelogenous leukemia blasts显示文摘 | Bruserud Φ Wendelbo Φ Paulsen K | | 0，，05： | 1 |
| 16 | Coculture of native human acute myelogenous leukemia blasts with fibroblasts and osteoblasts results in an increase of vascular endothelial growth factor levels显示文摘 | Glenjen NI Hatfield K Bruserud O | | 0，，01： | 1 |
| 17 | Flow cytometri meas- urement of apoptosis and necrosis in cryopreserved PBPC concentrates from patients with malignant diseases 显示文摘 | Abrahamsen JF Bakken AM Bruserud O | 2002 | Bone Marrow Transplant2002，29，2： | 1 |
| 18 | The chemokine system in experimental and clinical hematology显示文摘 | Bruserud Φ Kittang AO | | 0，，： | 1 |
| 19 | Leptin in human acute myelogenous leukemia:studies of in vivo levels and in vitro effects on native functional leukemia blasts 显示文摘 | Bruserud O Huang TS Glenjen N | 2002 | Haematologica2002，87，6： | 1 |
| 20 | Flow cytometric measurement of apoptosis and necrosis in cryopreserved PBPC concentrates from patients with malignant diseases 显示文摘 | ABRAHAMSEN JF BAKKEN AM BRUSERUD φ | 2002 | Bone Marrow Transplantation2002，29，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员