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| 1 | Characterization of spermidine hydroxycinnamoyl transferases from eggplant (Solanum melongena L.) and its wild relative Solanum richardii Dunal显示文摘Eggplant produces a variety of hydroxycinnamic acid amides(HCAAs)that have an important role in plant development and adaptation to environmental changes.In this study,we identified and characterized a spermidine hydroxycinnamoyl transferase(SHT)from eggplant(Solanum melongena)and its wild relative S.richardii,designated as SmSHT and SrSHT,respectively.SmSHT was abundant in flowers and fruits,whereas the level of SrSHT was remarkably low in all tissues.Heat-shock/drought treatment stimulated the expression of SmSHT in both leaves and fruits,indicating its involvement in plant stress response.Both SHT polypeptides had extremely high identity with just five amino-acid substitutions.Recombinant SmSHT catalyzed the synthesis of mono-,bi-and tri-acylated polyamines.Using caffeoyl-CoA as the acyl donor,SmSHT preferred spermidine as the acyl acceptor.When spermidine was the acyl acceptor,the donor preference order for SmSHT was caffeoyl-CoA>feruloyl-CoA>ρ-coumaroyl-CoA.SrSHT exhibited the same substrate specificity as SmSHT,yet exhibited significantly higher catalytic activity than SmSHT.For example,under caffeoyl-CoA and spermidine,Kcat of SrSHT was 37.3%higher than SmSHT.Molecular modeling suggests that five amino-acid substitutions in SrSHT result in four alterations in their predicted 3D structures.In particular,the conserved Lys402 adjacent to the DFGWG motif,and Cys200 in the crossover loop in SmSHT were replaced by Glu and Ser in SrSHT.These substitutions may contribute to the enhanced activity in SrSHT.Our study provides a platform to generate HCAA rich fruits for eggplant and other solanaceous crops. | Hui Peng Tianbao Yang Bruce D Whitaker Frances Trouth Lingfei Shangguan Wen Dong Wayne M Jurick | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Factors Affecting the Process of Collaborative Product Development显示文摘 | D Littler F Leverick M Bruce | 1995 | Journal of Product Innovation Management1995，12，1： | 1 |
| 3 | The Profession:Public Intrests andCommom Good显示文摘 | Bruce J Callahan D Wolf S M | 1987 | Hasting Center Report1987，，： | 1 |
| 4 | Factors affecting the process of collaborative product development: A study of U K manufacturers of information and communications technology products显示文摘 | Litter D Leverick F Bruce M | 1995 | Journal of Product Innovation Management1995，12，1： | 1 |
| 5 | Psychological disorders in patients with evacuation disorders and constipation in a tertiary practice显示文摘 | Nehra V Bruce B K Rath-Harvey D M | 2000 | Am J Gastroenterol2000，95，7： | 1 |
| 6 | Power and machinery: seed loss when cutting a standing crop of oilseed rape with two types of combine harvester header 显示文摘 | Hobson R N Bruce D M | 2002 | Biosystems Engineering2002，81，3： | 1 |
| 7 | Improving bleachability of radiate pine kraft pulps following treatment with hemicellulolytic enzymes显示文摘 | ClarkT A Steward D Bruce M E | 1991 | Appita1991，44，6： | 1 |
| 8 | Effect of textile properties in evalu- ating a directional shading fabric 显示文摘 | MAUREEN M G BRUCE D H ARDIS M R | 1997 | Textile Research Journal1997，67，4： | 1 |
| 9 | 查看详情显示文摘 | Bruce D A Wikinson A P White M G | | 0，，： | 1 |
| 10 | 查看详情显示文摘 | H L Nguyen P N Horton M B Hursthouse C Legon D W Bruce | | 0，，： | 1 |
| 11 | PPARγ: A nuclear regulator of metabolism, differentiation, and cell growth显示文摘 | Evan D R Bruce M S | 2001 | J Biol Chem2001，276，37： | 1 |
| 12 | The effects of the unemployment insurance payroll tax on wages, employment, claims and denials显示文摘 | Patricia M Anderson Bruce D Meyer | 2000 | Journal of Public Economics2000，，1： | 1 |
| 13 | DNA extraction from several cacti显示文摘 | Candelario M J Natalia D Bruce P B | 2000 | Hort Science2000，35，6： | 1 |
| 14 | COX-2 Inhibitors-Lessons in Drug Safety 显示文摘 | Bruce M Psaty Curt D Furberg | 2005 | N Engl J Med2005，352，： | 1 |
| 15 | Breeding oilseed rape for pod shattering resistance 显示文摘 | Morgan C L Ladbrooke Z L Bruce D M | 2000 | Journal of Agricultural Science2000，135，： | 1 |
| 16 | Improving bleachability of radiata pine kraft pulps following treatment with hemicellulolytic enzymes显示文摘 | Clark T A Steward D Bruce M E | 1991 | Appita1991，44，6： | 1 |
| 17 | Determining copper ions in water using electrochemiluminescence 显示文摘 | Bruce B Richter D M M | 2001 | Anal Chim Acta2001，449，： | 1 |
| 18 | Propylene Polymerization with Unbridge Metallocenes:Ligarid Effects in the Selectivity for Elastomeric Polypropylene显示文摘 | Maciejewsk J L Bruce P M D Waymouth R M | 1997 | Organometallics1997，16，： | 1 |
| 19 | Ineffectiveness of highdose methylprednisolone in preventing parenchymal lung injury and improv ing mortality in patients with septic shock显示文摘 | John M L Bruce A M James D M | 1988 | Am Rev Respir Dis1988，136，： | 1 |
| 20 | Diet aberrant crypt foci and colorectal cancer显示文摘 | BRUCE W R ARCHER M C CORPET D E | 1993 | Mutat Res1993，，290： | 1 |
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