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| 1 | Laser patterning for the study of MSC cardiogenic differentiation at the single-cell level显示文摘Mesenchymal stem cells(MSCs)have been cited as contributors to heart repair through cardiogenic differentiation and multiple cellular interactions,including the paracrine effect,cell fusion,and mechanical and electrical couplings.Due to heart–muscle complexity,progress in the development of knowledge concerning the role of MSCs in cardiac repair is heavily based on MSC–cardiomyocyte coculture.In conventional coculture systems,however,the in vivo cardiac muscle structure,in which rod-shaped cells are connected end-to-end,is not sustained;instead,irregularly shaped cells spread randomly,resulting in randomly distributed cell junctions.Consequently,contact-mediated cell–cell interactions(e.g.,the electrical triggering signal and the mechanical contraction wave that propagate through MSC–cardiomyocyte junctions)occur randomly.Thus,the data generated on the beneficial effects of MSCs may be irrelevant to in vivo biological processes.In this study,we explored whether cardiomyocyte alignment,the most important phenotype,is relevant to stem cell cardiogenic differentiation.Here,we report(i)the construction of a laser-patterned,biochip-based,stem cell–cardiomyocyte coculture model with controlled cell alignment;and(ii)single-cell-level data on stem cell cardiogenic differentiation under in vivo-like cardiomyocyte alignment conditions. | Zhen Ma Qiuying Liu Huaxiao Yang Raymond B Runyan Carol A Eisenberg Meifeng Xu Thomas K Borg Roger Markwald Yifei Wang Bruce Z Gao | 2013 | Light(Science & Applications)2013，2，1： | 2 |
| 2 | Modified Woodward procedure for Sprengel deformity of the shoulder: long - term results 显示文摘 | Borges JL Shah A Tortes BC | 1996 | J Pediatr Orthop1996，16，： | 1 |
| 3 | Determination of Lead in Biological Samples by High-resolution Continuum Source Graphite Furnace Atomic Absorption Spectrometry with Direct Solid Sampling显示文摘 | BORGES D G DA SILVA A F WELZ B | 2006 | JAnal at Spectrom2006，21，： | 1 |
| 4 | Re-Os isotopic systematics of primitive lavas from the Lassen region of the Cascade Arc,California显示文摘 | Brandon A D Clynne M A | 2000 | Earth and Planetary Science Letters2000，177，34： | 1 |
| 5 | Optimization of Diesel,Methyl Tallowate and Ethanol Blend for Reducing Emissions from Diesel Engine显示文摘 | Ali Y Hanna M A Borg J E | 1995 | Bioresource Technology1995，52，： | 1 |
| 6 | ERBB2 amplification is associated with tamoxifen resistance in steroid-receptor positive breast cancer显示文摘 | Borg A Baldetorp B Ferno M | 1994 | Cancer Lett1994，81，2： | 1 |
| 7 | Correlation of VEGF production with IL - 1α and IL - 6 secretion by human pituitary adenoma cells 显示文摘 | BORG S A KERRY K E ROYDS J A | 2005 | European Journal of Endocrinology2005，152，： | 1 |
| 8 | Recovery of impaired K ^+ channels in mesenteric arteries from spontaneously hypertensive rats by prolonged treatment with eholecalciferol 显示文摘 | Borges A C R Feres T Vianna L M | 1999 | Br J Pharmacol1999，127，： | 1 |
| 9 | Effect of resin cements and aging on cuspal deflection and fracture resistance of teeth restored with composite resin inlays显示文摘 | Salaverry A Borges GA Mota EG | 2013 | J Adhes Dent2013，15，6： | 1 |
| 10 | Selection of internal control genes for quantitative real-time RT-PCR studies during tomato development process 显示文摘 | Exposito-Rodriguez M Borges A A Borges-P6rez A | 2008 | BMC Plant Biology2008，8，1： | 1 |
| 11 | Assistive technology in devel- oping countries: a review from the perspective of the Conven- tion on the Rights of Persons with Disabilities 显示文摘 | Borg J Lindstr6m A Larsson S | 2011 | Prosthet Or- thot Int2011，35，1： | 1 |
| 12 | Spatial and temporal variation of phytoplankton in two subtropical Brazilian reservoirs 显示文摘 | BORGES P A F TRAIN S RODRIGUES L C | 2008 | Hydrobiologia2008，607，： | 1 |
| 13 | ERBIN:A basolateral PDZ protein that interacts with the mammalian ERBB2/HER2 receptor显示文摘 | Borg JP Marchetto S Le Bivic A | 2000 | Nature Cell Biol2000，2，： | 1 |
| 14 | Carbon nanotubes: solid-phase extraction显示文摘 | RAVELO-PEREZ L M HERERA-HERRERA A V HEMANDEZ- BORGES J | 2010 | J Chromatogr A2010，1217，16： | 1 |
| 15 | Immune response by nasal delivery of hepatitis B surface antigen and codelivery of a CpG ODN in alginate coated chitosan nanoparticles显示文摘 | Borges O Cordeiro-da-Silva A Tavares J | 2008 | EurJ Pharm Biopharm2008，69，2： | 1 |
| 16 | Clinical use of ultrasensitive cardiac troponin I assay in intermediate- and high-risk surgery patients 显示文摘 | Borges F K Furtado M V Rossini A P | 2013 | Dis Markers2013，35，6： | 1 |
| 17 | An efficient, thermally stable cerium-based silicate phosphor for solid state white lighting显示文摘 | Brgoch J Borg C K H Denanlt K A | 2013 | Inorg Chem2013，52，14： | 1 |
| 18 | sequencing hatch r and Biotechnology, Treat sludge eactor 显示文摘 | Schwarzenbeck N Borges J M Wilderer P A | 2005 | Applied Microbiology2005，66，6： | 1 |
| 19 | Tonin in rat heart with experimental hypertrophy显示文摘 | BORGES J C SILVA J A Jr GOMES M A | 2003 | Am J Physiol Heart Circ Physiol2003，284，6： | 1 |
| 20 | Psychophysical Bases of Perceived Exertion显示文摘 | Borg G A | 1982 | Med sci sports exerc1982，14，5： | 1 |
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