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| 1 | Highly efficient derivation of ventricular cardiomyocytes from induced pluripotent stem cells with a distinct epigenetic signature显示文摘从 pluripotent 干细胞导出的 Cardiomyocytes 能在药测试,疾病建模和基于房间的治疗被使用。没有 procardiogenic 生长因素,然而,从 pluripotent 干细胞的 cardiomyogenesis 的效率通常是低的,产生 cardiomyocyte 人口是异构的。这里,我们证明导致的 pluripotent 干细胞( iPSCs )能从鼠科的室的 myocytes ( VM )被导出,并且与从各种各样的体的房间类型导出的 iPSCs 的另外的报告一致,自发地作为与遗传上匹配的胚胎的干细胞(转换字符)或 iPSCs 相比区分 cardiomyocytes 进跳动的显著地更高的倾向从尾巴尖端成纤维细胞导出的 导出VM 的 iPSCs ( ViPSCs )展览。惊人地,导出 ViPSC 的 cardiomyocytes 显示的多数室的显型。在 ViPSCs 的提高的室的 myogenesis 在区别的早阶段经由心血管的祖先的增加的数字被调停。以便从 ViPSCs 调查提高的室的 myogenesis 的机制,我们执行了全球基因表示和 DNA methylation 分析,它揭示了可以涉及在 pluripotent 干细胞指定 VM 命运的不同 epigenetic 签名。 | Huansheng Xu B Alexander Yi Hao Wu Christoph Bock Hongcang Gu Kathy O Lui Joo-Hye C Park Ying Shao Alyssa K Riley Ibrahim J Domian Erding Hu Robert Willette John Lepore Alexander Meissner Zhong Wang Kenneth R Chien | 2012 | Cell Research2012，22，1： | 7 |
| 2 | Molecular phenotypes of human parvovirus B19 in patients with myocarditis显示文摘AIM:To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy(DCM).METHODS:Endomyocardial biopsies(EMBs) from 498 B19V-positive patients with myocarditis and DCMwere analyzed using molecular methods and functional experiments.EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers.Control tissues were obtained at autopsy from 34 victims of accidents,crime or suicide.Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining.Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay(ELISA).B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction(PCR).For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism(RFLP)-PCR was established.B19V-genotyping was verified by direct DNAsequencing and sequences were aligned using BLAST and BioEdit software.B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments.Transfection experiments were conducted using human endothelial cells 1.Luciferase reporter assays were performed to determine B19Vreplication activity.Statistical analysis and graphical representation were calculated using SPSS and Prism5 software.RESULTS:The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy(iCMP) compared to uninflamed DCM(59.6% vs 35.3%)(P < 0.0001).The detection of B19V-mRNA replication intermediates proved that replication of B19V was present.RFLP-PCR assays showed that B19V-genotype 1(57.4%) and B19V-genotype 2(36.7%) were the most prevalent viral genotypes.B19V-genotype 2 was observed more frequently in EMBs with iCMP(65.0%) compared to DCM(35%)(P = 0.049).Although there was no significant difference in gender-specific B19V-loads,women were more frequently infected with B19V-genotype 2(44.6%) than men(36.0%)(P = 0.0448).Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissuesamples and was associated with higher B19V viral load compared to B19V-monoinfected tissue(P = 0.0012).The most frequent coinfecting virus was human herpes virus 6(HHV6,16.5%).B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs(P = 0.0033),suggesting that HHV6 had transactivated B19V.In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator.CONCLUSION:The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP.Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype. | C-Thomas Bock Anja Düchting Friederike Utta Eva Brunner Bui Tien Sy Karin Klingel Florian Lang Meinrad Gawaz Stephan B Felix Reinhard Kandolf | 2014 | World Journal of Cardiology2014，6，4： | 3 |
| 3 | Induction of apoptosis in human blood T cells by 7,8-dihydroneopterin:the difference between healthy controls and patients with systemic lupus erythematosus 显示文摘 | Wirleitner B Obermoser G Bock G | 2003 | Clin Immunol2003，107，3： | 2 |
| 4 | General aspects and limits of conventional ultrafine WC powder manufacture and hard metal production显示文摘 | Schubert W D Bock A Lux B | 1995 | Int J Refract Met Hard Mat1995，13，： | 1 |
| 5 | Concomitant administration of varicella vaccine with combined measles,mumps and rubella vaccine in healthy children aged 12 to 24 months of age显示文摘 | Stuck B Stehr K Bock HL | | 0，，02： | 1 |
| 6 | Rocuronium potency and recovery characteristics during steady- state desflurane, sevoflurane, isoflurane or propofol anaesthesia 显示文摘 | Bock M Klippel K Nitsche B | 2000 | Br J Anaesth2000，84，1： | 1 |
| 7 | Semipermeable glove mem-branes-effects on skin barrier repair following SLS irritation 显示文摘 | Bock M Damer K Wulfhorst B | 2009 | Contact Dermatitis2009，61，5： | 1 |
| 8 | Sensitive and specific detection of proviral bovine leukemia virus by 5' Taq nuclease PCR using a 3' minor groove binder fluorogenic probe 显示文摘 | Lew A E Bock R E Molloy J B | 2004 | J Virol Methods2004，115，2： | 1 |
| 9 | General aspects and limits of conventional ultrafine WC powder manufacture an hard metal production显示文摘 | Schubert W D Bock A Lux B | 1995 | Int J Refract Met Hard Mater1995，13，5： | 1 |
| 10 | Validation of the 4B5 rabbit monoclonal antibody in determining HER-2/neu status in breast cancer显示文摘 | van der Vegt B de Bock G H Bart J | 2009 | Mod Pathol2009，22，7： | 1 |
| 11 | Response of spring wheat to nitrogen fertilizers of different nitrification rates显示文摘 | Goos R J Schimelfenig J A Bock B R | 1999 | Agronomy Journal1999，91，2： | 1 |
| 12 | Demonstration of earth- quake early warning using total displacement waveforms from real-time GPS networks显示文摘 | Crowell B W Y Bock M B Squibb | 2009 | Seismological Research Letters2009，80，5： | 1 |
| 13 | In vitro corneal permeability of diclofenac sodium in formulations containing cyclodextrins compared to the commercial product voltaren ophtha 显示文摘 | Reer O Bock T K Muller B W | 1994 | Journal of Pharmaceutic Sciences1994，83，9： | 1 |
| 14 | Assessment ofEutrophication in the Firth of Clyde:Analysis of CoastalWater Data from 1982 to 1996显示文摘 | Bock M T Miller B S Bowma A W | 1999 | Marine PollutionBulletin1999，38，3： | 1 |
| 15 | Brain Research显示文摘 | Kφhler L B Berezin Vladimir Bock Elisabeth Milena Penkowa | 2003 | 992(1): 1282003，992，1： | 1 |
| 16 | N2O and NO emissions from poultry litter and urea applications to bermuda grass 显示文摘 | Thornton F C Shurpali N J Bock B R | 1998 | A tmos Environ1998，32，9： | 1 |
| 17 | Use of high speed selective jet electrodeposition of gold for lhe plating of connectors显示文摘 | Bocking C Cameron B | 1994 | Trmsactions of the Institute of Metal Finishing1994，72，1： | 1 |
| 18 | General aspects and limits of conventional ultra_fine WC powder manufacture and hard metal production 显示文摘 | Shubert W D Bock A Lux B | 1995 | International Journal of Refractory Metals &Hard Matcrials1995，13，5： | 1 |
| 19 | General aspects and limits of conventional ultrafine WC powder manufacture and hard metal production显示文摘 | SCHUBERT W D BOCK A LUX B | 1995 | International Journal of Refractory Metals & Hard Materials1995，13，： | 1 |
| 20 | Proliferation-dependent alterations of the DNA methylation landscape underlie hematopoietic stem cell aging显示文摘 | Beerman I Bock C Garrison B S | 2013 | Cell Stem Cell2013，12，4： | 1 |
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