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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | Dietary self-efficacy:Determinant of compliance behaviors and biochemical outcomes in hemodialysis patients显示文摘 | Zrinyi M Juhasz M Balla J | 2003 | Nephrol Dial Transplant2003，18，： | 1 |
| 3 | Postoperative adhesion prevention using a statin-containing cellulose film in an experimental model显示文摘 | Lalountas M Ballas K D Michalakis A | | 0，，： | 1 |
| 4 | Relapsing polychondritis with p-ANCA associated vasculitis: Which triggers the other?显示文摘Relapsing polychondritis(RP) is a rare autoimmune disease with chronic inflammatory/destructive lesions of the cartilaginous tissues. In one third of the cases it is associated with other autoimmune disorders, mostly with anti-neutrophil cytoplasmic antibody(ANCA) associated vasculitis(AAV). We report three cases of RP with p-ANCA positive AAV. In the first patient RP developed 1.5 years after the onset of AAV. In the others the signs of RP were present before the onset of severe crescent glomerulonephritis. Patients responded well on steroid and cyclophosphamide. In dialysis dependent cases plasmapheresis was also used successfully. During the 2 and 1.5 years of follow up, they were symptom-free, and had stable glomerular filtration rate. The first patient died after four years of follow-up due to the complications of sudden unset pancytopenia,which raises the possibility of associated hemophagocytic syndrome. In the setting of RP or AAV physicians should always be aware of the possibility of sudden or insidious appearance of the other disease. | Ibolya File Csilla Trinn Zsolt Mátyus László Ujhelyi József Balla János Mátyus | 2014 | World Journal of Clinical Cases2014，2，12： | 1 |
| 5 | New-onset diabetes after transplantation : a review of recent literature显示文摘 | Balla A Chobanian M | 2009 | Curr Opin Organ Transplant2009，14，4： | 1 |
| 6 | Analytical study of the thermal shock prob- lem of a half-space with various thermoelastic models显示文摘 | BALLA M | 1991 | ActaMeehaniea1991，89，1234： | 1 |
| 7 | Ferritin:a cytoprotective antioxidant stratagem of endothelium 显示文摘 | Balla G Jacob HS Balla J Rosenberg M Nath K Apple F | 1992 | J Biol Chem1992，257，18： | 1 |
| 8 | Additive manufac- turing of Co-Cr-Mo alloy: influence of heat treatment on mi- erostructure, tribological, and electrochemical properties 显示文摘 | Mantrala M K Das M Balla V K | 2015 | Frontiers in Mechanical Engineering2015，，1： | 1 |
| 9 | Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA显示文摘 | Ballas Z K Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，5： | 1 |
| 10 | Immunostimulatory oligodeoxynucleotides containing CpG motifs enhance the efficacy of monoclonalo antibody therapuy of lymphoma显示文摘 | Wooldridge J E Ballas Z Krieg A M | 1997 | Blood1997，89，： | 1 |
| 11 | Differential activity of catalase and superoxide dismutase in seedlings and in vitro microprop agated oak 显示文摘 | Rrcchi M L Bagnoli F Balla I | 2001 | Plant Cell Reports2001，20，： | 1 |
| 12 | Early-onset carotid athero-sclerosis is associated with increased intima-media thickness and elevated serum levels of inflammatory markers显示文摘 | Magyar M T Szikszai Z Balla J | 2003 | Stroke2003，34，1： | 1 |
| 13 | Analytical study of the thermal shock problem of a haft-space with vari-ous thermoelastic models显示文摘 | Balla M Hungary B | 1991 | Acta Mechanica1991，89，14： | 1 |
| 14 | Heine,heme oxy-genase and ferritin in vascular endothelial cell injury显示文摘 | BALLA J VERCELLOTTI G M JENEY V | 2005 | Mol Nutr Food Res2005，49，： | 1 |
| 15 | Canalization of auxin flow by Aux/IAA-ARF-dependent feedback regulation of PIN polarity 显示文摘 | SAUER M BALLA J LUSCHNIG C | 2006 | Genes g Development2006，20，20： | 1 |
| 16 | Sertoli cell proliferation in the fetal and neonatal rattestis:a continuous phenomenon显示文摘 | ANGELOPOULOU R BALLA M LAVRANOS G etal | 2008 | Acta Histochem2008，110，4： | 1 |
| 17 | Microwave-sin- tered 3D printed tricalcium phosphate scaffolds for bone tis- sue engineering 显示文摘 | Tarafder S Balla V K Davies N M | 2013 | J Tissue Eng Regen M2013，7，8： | 1 |
| 18 | Dietary self-efficacy : Determinant of compliance behaviours and biochemical outcomes in hemodialysis patients显示文摘 | Zrinyi M J uhasz M Balla J | 2003 | Nephrol Dial Transplant2003，18，： | 1 |
| 19 | Induction of NK activity in murine and human cells by CpG motifys in oligodoxynucleotides and bacterial DNA显示文摘 | Ballas Z K Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，5： | 1 |
| 20 | merican society ofhospital pharmacists ASHP technical assistance bulletin onhandling cytotoxic and hazardous drugs显示文摘 | Krieg A M Ballas Z K Rasmussen W L | 1990 | Am JhospPharm1990，47，8： | 1 |
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