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| 1 | Interaction of hepatitis C virus envelope glycoprotein E2 with the large extracellular loop of tupaia CD81显示文摘AIM: To further analyze the interaction of tupaia CD81 with hepatitis C virus (HCV) envelope protein E2. METHODS: A tupaia CD81 large extracellular loop (CD81 LEL), which binds to HCV E2 protein, was cloned and expressed as a GST-fusion protein, and interaction of HCV E2 protein with a tupaia CD81 LEL was evaluated by enzyme-linked immunosorbent assay (EIA). RESULTS: Although tupaia and human CD81 LEL differed in 6 amino acid changes, tupaia CD81 LEL was strongly recognized by anti-CD81 antibodies against human CD81 LEL conformation-dependent epitopes. Investigating LEL CD81-E2 interactions by EIA, we demonstrated that binding of tupaia CD81 LEL GST fusion protein to recombinant HCV E2 protein was markedly reduced compared to binding of human CD81 LEL GST fusion protein to recombinant HCV E2 protein. CONCLUSION: These data suggest that the structural differences in-between the tupaia and human CD81 may alter the interaction of the large extracellular loop with HCV envelope glycoprotein E2. These findings may be important for the understanding of the mechanisms of binding and entry of HCV to PTHs. | Zhan-Fei Tian Hong Shen Xi-Hua Fu Yi-Chun Chen Hubert E Blum Thomas F Baumert Xi-Ping Zhao | 2009 | World Journal of Gastroenterology2009，15，2： | 16 |
| 2 | Pseudomonas exotoxin antisense RNA selectively kills hepatitis B virus infected cells显示文摘AIM: To present an approach for selectively killing retrovirus-infected cells that combines the toxicity of Pseudomonas exotoxin (PE) and the presence of reverse transcriptase (RT) in infected cells. METHODS: PE antisense toxin RNA has palindromic stem loops at its 5' and 3' ends enabling self-primed generation of cDNA in the presence of RT. The RT activity expressed in retrovirus-infected cells converts 'antisense-toxin-RNA' into a lethal toxin gene exclusively in these cells. RESULTS: Using cotransfection studies with PE-expressing RNAs and β-gal expressing reporter plasmids, we show that, in HepG2 and HepG2.2.15 hepatoma cells as well as in duck hepatitis B virus (DHBV) infected cells, HBV or DHBV-polymerase reverse transcribe a lethal cDNA copy of an antisense toxin RNA, which is composed of sequences complementary to a PE gene and eukaryotic transcription and translation signals. CONCLUSION: This finding may have important implications as a novel therapeutic strategy aimed at the elimination of HBV infection. | Peter Hafkemeyer Ulrich Brinkmann Elizabeth Brinkmann Ira Pastan Hubert E Blum Thomas F Baumert | 2008 | World Journal of Gastroenterology2008，14，18： | 2 |
| 3 | Polycyclic aromatic hydrocarbons,black carbon,and molecular markers in soils of Switzerland显示文摘 | BUCHELI T D BLUM F DESAULES A | 2004 | Chemosphere2004，56，11： | 1 |
| 4 | Intracellular localization and in vivo trafficking of p24A and p23显示文摘 | Blum R Pfeiffer F Feick P | | 0，，： | 1 |
| 5 | Effects of soy protein on endothelium-dependent vasodilatation and lipid profile in postmenopausal women with mild hypercholesterolemia 显示文摘 | Blum A Lang N Vigder F | 2003 | Clinical and Investigative Medicine-Medecine Cli- nique Et Experimentale2003，26，1： | 1 |
| 6 | Biomechanical comparison of bending and torsional properties in retrograde intramedullary nailing of humeral shaft fractures显示文摘 | Blum J Machemer H Baumgart F | 1999 | J Orthop Trauma1999，13，： | 1 |
| 7 | 10-hydroxy-delta 2-decdnoic acid, an antibiotic found in royal jelly显示文摘 | BLUM M S NOVAK A F TABER S | 1959 | Science1959，130，3373： | 1 |
| 8 | Renal slit diaphragm-the open zipper and the failing heart显示文摘 | Blum S Nakhoul F Khankin E | 2007 | Isr Med Assoc J2007，9，2： | 1 |
| 9 | Contribution of and vogens to the gender difference in leptin production in obese children and adoles - cents显示文摘 | Wabitsch M Blum W F Muche R | 1997 | J Clin Znest1997，100，： | 1 |
| 10 | A cascadic multigrid algorithm for variational inequalities显示文摘 | BLUM H BRAESS D SUTTMEIER F T | 2004 | Com- puting and Visualization in Science2004，7，3： | 1 |
| 11 | Effect of interfacial mobility on flexural strength and fracture toughness of glass/ epoxy laminates 显示文摘 | Wang T W H Blum F D Dharani L R | 1999 | Journal of Materials Science1999，，34： | 1 |
| 12 | Contribution of androgens to the gender difference in leptin production in obese children and adolescents显示文摘 | WABITTSCH M BLUM W F MUCHE R | 1997 | J Clin Invest1997，100，： | 1 |
| 13 | Adsorption of sodium - decylbenze- nesulfonate onto alumina: relationships between macro - and microscopic behavior 显示文摘 | Nagashima K Blum F D | 1999 | Journal of Colloid and Interface Science1999，214，1： | 1 |
| 14 | Performance of magnetic resonance angiography in suspected acute pulmonary embolism 显示文摘 | Blum A Bellou A Guillemin F | 2005 | Thromb Haemost2005，93，50： | 1 |
| 15 | Boron and lithium isotopes as groundwater tracers:a study at the Fresh Kills Landfill,State Island,New York,USA显示文摘 | Hogan J F Blum O D | 2003 | Applied Geochemistry2003，18，： | 1 |
| 16 | Relative taste potency of some basic food constituents and their competitive and compensatory action显示文摘 | Fabian F W Blum H B | 1942 | Food research1942，8，3： | 1 |
| 17 | A mitogen-activated protein kinase cascade in the CA1/CA2 subfield of the dorsal hippocampus is essential for long-term spatial memory 显示文摘 | Blum S Moore AN Adams F etal | 1999 | J Neurosci1999，19，9： | 1 |
| 18 | Patient mount an autoimmune response to their bladder cancer-associated T antigen显示文摘 | Ignatoff J M Springer G F Blum M D | 1983 | Naturwissenschaften1983，70，1： | 1 |
| 19 | A mitogen-activated protein kinase cascade in the CA1/CA2 subfield of the dorsal hippocampus is essential for long-term spatial memory显示文摘 | Blum S Moore AN Adams F | 1999 | J Neurosci1999，19，9： | 1 |
| 20 | PA mitogenactivated protein kinase cascade in the CA1/CA2subfield of the dorsal hippocampus is essential for longterm spatial memory显示文摘 | Blum S Moore AN Adams F | 1999 | J Neurosci1999，19，9： | 1 |
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