|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | An epigenomic approach to therapy for tamoxifen-resistant breast cancer显示文摘Tamoxifen 是为雌激素受体 alpha 的前线治疗(ERα) 在绝经前的女人的积极的胸肿瘤。然而,到 tamoxifen 的抵抗发生在许多病人。嗯仍然与获得的 tamoxifen 抵抗在乳癌房间的生长起一个关键作用,建议那 ERα为治疗仍然是一个有效目标 tamoxifen 抵抗(Tam-R ) 乳癌。以识别 ERα 的新奇管理者;发信号,通过对 histone 甲基修饰词的一幅小规模的 siRNA 屏幕,我们发现了 WHSC1, histone H3K36 methyltransferase, ERα 的一个积极管理者;在乳癌房间发信号。我们证明 WHSC1 被招募到 ERα由 BET 蛋白质 BRD3/4 的基因,并且便于 ERα基因表示。小分子的赌注蛋白质禁止者 JQ1 potently 压制了经典 ERα发信号的小径和在文化的 Tam-R 乳癌房间的生长。用一个 Tam-R 乳癌异种皮移植老鼠模型,我们与 JQ1 和 ER degrader fulvestrant 由 JQ1 和联合治疗的强壮的长持续的效果在 vivo 反胸癌症活动示威了。一起拿,我们提供 epigenomic 蛋白质 BRD3/4 和 WHSC1 是雌激素受体发信号的必要管理者并且是为 Tam-R 乳癌的治疗的新奇治疗学的目标的证据。 | Qin Feng Zheng Zhang Martin J Shea Chad J Creighton Cristian Coarfa Susan G Hilsenbeck Rainer Lanz Bin He Lei Wang Xiaoyong Fu Agostina Nardone Yongcheng Song James Bradner Nicholas Mitsiades Constantine S Mitsiades C Kent Osborne Rachel Schiff Bert W O'Malley | 2014 | Cell Research2014，24，7： | 10 |
| 2 | Raman spectroscopy employed for the determination of the intermediate phase in polyethylene显示文摘 | CHRISTOPHER C N ROBERT J M BERT J K et al | 1995 | Macromolecules1995，28，8： | 1 |
| 3 | Selenium redox biochemistry of zinc - sulfur co- ordination sites in proteins and enzymes显示文摘 | Claus J wolfgang M Bert V | 1999 | Proc Natl Acad Sci USA1999，96，： | 1 |
| 4 | Continuous fractional crystallization显示文摘 | W D Bert J W Freemall D McNeil | 1968 | J Appl Chem1968，18，6： | 1 |
| 5 | Vibration of thick rectangular plates of bimodulous composite material显示文摘 | Bert C W Reddy J N Chao W C | 1981 | Journal of Applied Mechanics1981，48，2： | 1 |
| 6 | Rules, discretion and reputation in a model of monetary policy 显示文摘 | R-O'BERT J BARRO DAVID B GORDON | 1983 | Journal of Monetary Eeonmics1983，，12： | 1 |
| 7 | Optimization of biolistic transformation of embryogenic grape cell suspensions显示文摘 | H(e)bert D Kikkert J R Smith F D Reisch B I | | 0，，： | 1 |
| 8 | Influenza viral neuraminidase: the for- gotten antigen显示文摘 | BERT E J MANON MJC | 2011 | Expert Rev Vaccines2011，10，12： | 1 |
| 9 | Solution of the improved and generalized quasistatic methods using an analytic calculation or a semiimplicit scheme to compute the precursor equations显示文摘 | KOCLAS J SISSAOUI M T H BERT A | 1996 | Ann Nucl Energy1996，23，11： | 1 |
| 10 | Agenoralized model for the prediction of lead body burdens 显示文摘 | BERT J L Van DUSEN L J GRACE J E | 1989 | Environ Res1989，48，： | 1 |
| 11 | Sphere-forming corneal cells repopulate dystrophic keratoconic stroma:Implications for potential therapy显示文摘BACKGROUND Keratoconus is a degenerative corneal disease characterised by aberrant cell behaviour and loss of matrix that can result in vision loss.Cells extracted from peripheral corneas can form stem cell-enriched spheres,which have shown the potential to repopulate the normal peripheral corneal stroma in vitro upon sphere implantation but have not been previously studied in keratoconic tissue.AIM To investigate the therapeutic potential of stem cell-enriched spheres formed from extracted peripheral human corneal cells when introduced to keratoconic tissue.METHODS Stem cell-enriched spheres were formed from extracts of normal cadaveric human peripheral corneal cells.These spheres were implanted into incisions created in full thickness and onto the surface of 10μm thin sections of keratoconic and normal stromal tissues in vitro.Tissue sections were used to maximise use of limited keratoconic tissue available for research.Living cells were stained with Calcein-AM and visualised with stereo and fluorescence microscopy to assess survival and behaviours between the time of implantation day 0 and 14 d(D14)from implantation.Sphere cells in implanted tissues were characterised for stem cell and differentiation markers using immunohistochemistry and droplet digital PCR to assess the potential implications of these characteristics in the use of spheres in keratoconus treatment.RESULTS Spheres were successfully implanted into full-thickness central corneal tissue and onto the surface of 10μm thin en face tissue sections.No observable differences were seen in sphere migration,proliferation or differentiation in keratoconic tissue compared to normal between day 0 and D14.Spheres stained positively with Calcein-AM up to D14.Cell migration increased from day 0 to D14,occurring radially in three dimensions from the sphere and in alignment with tissue edges.Cell proliferation marker,EdU,was detected at day 10.Implanted spheres stained positively for putative stem cell markersΔNp63αand ABCB5,while ABCG2,ABCB5,ΔNp63 and p63αwere detectable by droplet digital PCR up to D14.Double immunolabelling revealed absence of ABCB5 staining in migrated cells but positive staining of alpha smooth muscle actin(myofibroblast marker)in some migrated cells.Droplet digital PCR showed similar expression patterns of differentiation markers but a reduction in stem cell markers between normal and keratoconic tissue with an increase in stromal cell markers and a reduction in epithelial cell markers,indicating an appropriate response to repopulating diseased tissue.CONCLUSION Cells from implanted stem cell-enriched spheres can repopulate a keratoconic corneal stromal surface in a directed manner and exhibit migratory stromal cell phenotypes. | Himanshu Wadhwa Salim Ismail Jennifer J McGhee Bert Van der Werf Trevor Sherwin | 2020 | World Journal of Stem Cells2020，12，1： | 1 |
| 12 | Heme oxygenase-1 expression inhibits dendritic cell maturation and proinflammatory function but conserves IL 10 expression显示文摘 | Chauveau C Remy S Royer P J Hill M Tanguy Royer S Hu- bert F X | 2005 | Blood2005，106，： | 1 |
| 13 | Switching species tropism: an effective way to manipulate the feline coronavirus genome 显示文摘 | BERT J H HAUKELIENE V PETER J M | 2003 | Journal of Virology2003，77，8： | 1 |
| 14 | The miR - 15/107 group of microRNA genes: evolutionary biology, cellular functions, and roles in human diseases显示文摘 | FINNERTY J R WANG W X HI'BERT S S | 2010 | J Mol Biol2010，402，3： | 1 |
| 15 | Mi-croRNAs as regulators of epithelial-mesenchymal tran-sition显示文摘 | Gregory P A Bracken C P Bert A G Goodall G J | 2008 | Cell Cycle2008，7，： | 1 |
| 16 | Pressure-volume relationship for rat dermis: Compression studies显示文摘 | Bert J L Reed R K | 1997 | Acta physiologica Scan- dinavica1997，160，1： | 1 |
| 17 | A new classifica- tion of thoracolumbar injuries: the importance of injury morphology, the in- tegrity of the posterior ligamentous complex, and neurologic status 显示文摘 | Vaccaro AR Lehman RA Hur|bert J | 2005 | Spine2005，20，： | 1 |
| 18 | A multiresolution spline with application to image mosaics 显示文摘 | Bert P J Adelson E H | 1983 | ACM Transactions on Graphics1983，2，4： | 1 |
| 19 | Carbon and nutriem losses during manure storage under traditional and improved prac- tices in smallholder crop - livestock systems-evidence from Kenya 显示文摘 | Pablo T Mariana C R Bert H J | 2011 | Plant and Soil2011，328，： | 1 |
| 20 | The role of waterlogging in maintaining forb pannes in northern New England salt marshes 显示文摘 | Ewanchuk P J Bertness M D | 2004 | Journal of Ecology2004，85，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员