|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy显示文摘Dormancy-associated MADS-box(DAM)genes serve as crucial regulators of the endodormancy cycle in rosaceous plants.Although pear DAM genes have been identified previously,the lack of a high-quality reference genome and techniques to study gene function have prevented accurate genome-wide analysis and functional verification of such genes.Additionally,the contribution of other genes to the regulation of endodormancy release remains poorly understood.In this study,a high-quality genome assembly for'Cuiguan'pear(Pyrus pyrifolia),which is a leading cultivar with a low chilling requirement cultivated in China,was constructed using PacBio and Hi-C technologies.Using this genome sequence,we revealed that pear DAM genes were tandemly clustered on Chr8 and Chr15 and were differentially expressed in the buds between'Cuiguan'and the high-chilling-requirement cultivar'Suli'during the dormancy cycle.Using a virus-induced gene silencing system,we determined the repressive effects of DAM genes on bud break.Several novel genes potentially involved in the regulation of endodormancy release were identified by RNA sequencing and H3K4me3 chromatin immunoprecipitation sequencing analyses of‘Suli'buds during artificial chilling using the new reference genome.Our findings enrich the knowledge of the regulatory mechanism underlying endodormancy release and chilling requirements and provide a foundation for the practical regulation of dormancy release in fruit trees as an adaptation to climate change. | Yuhao Gao Qinsong Yang Xinhui Yan Xinyue Wu Feng Yang Jianzhao Li Jia Wei Junbei Ni Mudassar Ahmad Songling Bai Yuanwen Teng | 2021 | Horticulture Research2021，8，1： | 5 |
| 2 | Bud endodormancy in deciduous fruit trees:advances and prospects显示文摘Bud endodormancy is a complex physiological process that is indispensable for the survival,growth,and development of deciduous perennial plants.The timely release of endodormancy is essential for flowering and fruit production of deciduous fruit trees.A better understanding of the mechanism of endodormancy will be of great help in the artificial regulation of endodormancy to cope with climate change and in creating new cultivars with different chilling requirements.Studies in poplar have clarified the mechanism of vegetative bud endodormancy,but the endodormancy of floral buds in fruit trees needs further study.In this review,we focus on the molecular regulation of endodormancy induction,maintenance and release in floral buds of deciduous fruit trees.We also describe recent advances in quantitative trait loci analysis of chilling requirements in fruit trees.We discuss phytohormones,epigenetic regulation,and the detailed molecular network controlling endodormancy,centered on SHORT VEGETATIVE PHASE(SVP)and Dormancy-associated MADS-box(DAM)genes during endodormancy maintenance and release.Combining previous studies and our observations,we propose a regulatory model for bud endodormancy and offer some perspectives for the future. | Qinsong Yang Yuhao Gao Xinyue Wu Takaya Moriguchi Songling Bai Yuanwen Teng | 2021 | Horticulture Research2021，8，1： | 2 |
| 3 | Efficient Breeding and Cultivation of Type 2 Red-fleshed Apple Cultivars Using a Search System for Suitable Apple Cultivar Combination显示文摘As apple possesses self-incompatibility, knowledge of the S-genotypes is very important for apple breeding and cultivation. We investigated the S-RNase genotypes of 13 type 2 red-fleshed apple cultivars and 8 lineages. All of the investigated type 2 red-fleshed apples contained the S3-RNase allele closely linked to the red-fleshed trait. We made a search system for ovule parents to efficiently produce new type 2 red-fleshed cultivars based on the S-RNase allele information(http://gffzzd3a1d37479ed4c08hu95fnuokkvqq6px6.ffgz.tsg.suse.edu.cn/~hort/apple/red/). More than 90% of progenies from crossing a selected white-fleshed ovule parent with a type 2 red-fleshed pollen parent are expected to show the red-fleshed phenotype. We also compiled a database of the S-RNase genotypes of more than 1 000 apple cultivars, including a survey system of cultivar combinations showing those that were fully incompatible, semicompatible and fully compatible, written in Chinese(http://gffzzd3a1d37479ed4c08hu95fnuokkvqq6px6.ffgz.tsg.suse.edu.cn/~hort/apple/ch/) and Japanese(http://gffzzd3a1d37479ed4c08hu95fnuokkvqq6px6.ffgz.tsg.suse.edu.cn/~hort/apple/ja/). | SHOGO Matsumoto LI Tianzhong SHUNGO Otagaki LI Yang BAI Songling | 2018 | Horticultural Plant Journal2018，4，6： | 2 |
| 4 | Vitamin B6 regulates IL-33 homeostasis to alleviate type 2 inflammation显示文摘Interleukin-33(IL-33)is a crucial nuclear cytokine that induces the type 2 immune response and maintains immune homeostasis.The fine-tuned regulation of IL-33 in tissue cells is critical to control of the type 2 immune response in airway inflammation,but the mechanism is still unclear.Here,we found that healthy individuals had higher phosphate-pyridoxal(PLP,an active form of vitamin B6)concentrations in the serum than asthma patients.Lower serum PLP concentrations in asthma patients were strongly associated with worse lung function and inflammation.In a mouse model of lung inflammation,we revealed that PLP alleviated the type 2 immune response and that this inhibitory effect relied on the activity of IL-33.A mechanistic study showed that in vivo,pyridoxal(PL)needed to be converted into PLP,which inhibited the type 2 response by regulating IL-33 stability.In mice heterozygous for pyridoxal kinase(PDXK),the conversion of PL to PLP was limited,and IL-33 levels were increased in the lungs,aggravating type 2 inflammation.Furthermore,we found that the mouse double minute 2 homolog(MDM2)protein,an E3 ubiquitin-protein ligase,could ubiquitinate the N-terminus of IL-33 and sustain IL-33 stability in epithelial cells.PLP reduced MDM2-mediated IL-33 polyubiquitination and decreased the level of IL-33 through the proteasome pathway.In addition,inhalation of PLP alleviated asthma-related effects in mouse models.In summary,our data indicate that vitamin B6 regulates MDM2-mediated IL-33 stability to constrain the type 2 response,which might help develop a potential preventive and therapeutic agent for allergy-related diseases. | Songling Zhu Shufen Zhong Kebin Cheng Li-Sha Zhang Jiu-wu Bai Zu Cao Su Wang Wen Chen Shipeng Cheng Liyan Ma Zhiyang Ling Yuying Huang Wangpeng Gu Xiaoyu Sun Chunyan Yi Meng Zhao Shuo Liang Jin-Fu Xu Bing Sun Yaguang Zhang | 2023 | Cellular & Molecular Immunology2023，20，7： | 1 |
| 5 | Recent advances in organic and polymeric carriers for local tumor chemo-immunotherapy显示文摘Combination therapy involves the simultaneous administration of compounds with varying mechanisms of action that can improve the efficacy of antitumor therapy and reduce toxicity.The most widely used combination regimen is chemotherapy combined with focused immunotherapy.This is implemented to induce the apoptosis of tumor cells and can activate immune responses,improving the clearance rate of primary lesions and maintaining the resistance to postoperative tumor recurrence and metastasis.Advances in micro/nanotechnology,nanomedicine and biomaterials have contributed to the development of enhanced local drug co-delivery systems for cancer treatment,improving tumor targeting and ameliorating severe systemic complications.Carrier materials can achieve the local long-term controllable release of multiple drugs,which not only avoids rapid drug diffusion from the pathological site,but can achieve synergistic effects at lower drug concentrations.Polymeric carriers display excellent biocompatibility and biodegradability;especially,some of them also have anti-tumor effects.The aim of this article was to review recent progress in the use of organic and polymeric materials for local tumor chemo-immunotherapy,which can be used as carriers for chemotherapeutic drugs,immune adjuvants and genes,including amphiphilic nanoparticles,nanocapsules,nano-disks,nano-polyplex particles,hydrogels and implantable materials. | BAI YiTing WANG TianRan ZHANG SongLing CHEN XueSi HE ChaoLiang | 2022 | Science China(Technological Sciences)2022，65，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员