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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 3 | Rate constants of reaction of ozone with organic and inorganic compounds in water-I:Nondissociating organic compounds 显示文摘 | Hoigne J Bader H | | Water Res0，17，2： | 1 |
| 4 | Rate constants of reactions of ozone with organic and inorganic compounds in water-I : non-dissociating organic compounds 显示文摘 | Hoigne J Bader H | 1983 | Water Res1983，17，2： | 1 |
| 5 | Tongue protrusion and feeding dystonia: a hallmark of chorea-acanthocytosis显示文摘 | Bader B Walker R H Vogel M | 2010 | Mov Disord2010，25，1： | 1 |
| 6 | Rate constants of reactions of ozone with organic and inorganic compounds in water-II: dissociating organic compounds 显示文摘 | Hoigne J Bader H | 1983 | Water Res1983，17，2： | 1 |
| 7 | Ablation dynamics of periodic nanostructures for polymer-based all-optical devices 显示文摘 | Klein-Wiele J H Bader M A Bauer I | 2002 | Synthetic Metals2002，127，： | 1 |
| 8 | Dynamic characterization of human tendons显示文摘 | Schechtman H Bader DL | | 0，，： | 1 |
| 9 | Driver behavior du- ring flashing green before amber: a comparative study 显示文摘 | Koll H Bader M Axhuasen K W | 2004 | Accident Analysis and Prevention2004，36，： | 1 |
| 10 | Comparison of bupivacaine and ropivacaine induced conduction blockade in the isolated rabbit vagus nerve显示文摘 | Bader AM Datta S Flanawgan H | 1989 | Anesth Analg1989，68，11： | 1 |
| 11 | Treatment of perennid non- allergic rhinopathy with capsaicin 显示文摘 | Riechelmann H Davris S Bader D | 1993 | HNO1993，41，10： | 1 |
| 12 | In vitro fatigue of human tendons显示文摘 | Schechtman H Bader DL | | 0，，08： | 1 |
| 13 | Determination of ozone in water by the indigo method显示文摘 | BADER H HOIGNJ | 1981 | Water Research1981，15，4： | 1 |
| 14 | The hyperbolic distribution of particle sizes 显示文摘 | Bader H | 1970 | J Geophys Res1970，75，： | 1 |
| 15 | Rate constants of reactions of ozone with organic and inorganic compounds in water- Ⅱ: dissociating organic compounds 显示文摘 | HOIGNE J BADER H | 1983 | Water Research1983，17，2： | 1 |
| 16 | Bi directional holographic information storage based on the optical reorientation of FA centers in KCl : Na显示文摘 | BLUME H BADER L LUTY F | 1974 | Opt Commun1974，12，2： | 1 |
| 17 | In vitro activities of benzimidazoles against Echinococcus muhilocularis metacestodes显示文摘 | Jura H Bader A Frosch M | 1998 | J Antimierob Agents Chemother1998，42，5： | 1 |
| 18 | The flow of phosphorous in food production and consumption-Linkoping,Sweden, 1870- 2000 显示文摘 | Neset T S S Bader H P Scheidegger R | 2008 | Science of the Total Environment2008，396，23： | 1 |
| 19 | Determination of ozone in water by the indigomethod显示文摘 | Bader H HoignéJ | 1981 | Water Research1981，15，4： | 1 |
| 20 | The Role of Hydroxyl Radi- cal Reactions in Ozonation Processes in Aqueous Solu tions显示文摘 | HOIGNE J BADER H | 1976 | Water Research1976，10，2： | 1 |
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