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| 1 | Inhibition of IKK-NFκB pathway sensitizes lung cancer cell lines to radiation显示文摘Objective:Cancer cell radioresistance is a stumbling block in radiation therapy.The activity in the nuclear factor kappa B(NFκB)pathway correlates with anti-apoptotic mechanisms and increased radioresistance.The IKK complex plays a major role in NFκB activation upon numerous signals.In this study,we examined the interaction between ionizing radiation(IR)and different members of the IKK-NFκB pathway,as well as upstream activators,RAF1,ERK,and AKT1.Methods:The effect of 4 Gy of IR on the expression of the RAF1-ERK-IKK-NFκB pathway was examined in A549 and H1299 lung cancer cell lines using Western blot analysis and confocal microscopy.We examined changes in radiation sensitivity using gene silencing or pharmacological inhibitors of ERK and IKKβ.Results:IKKα,IKKγ,and IκBαincreased upon exposure to IR,thereby affecting nuclear levels of NFκB(phospho-p65).ERK inhibition or si RNA-mediated down-regulation of RAF1 suppressed the post-irradiation survival of the examined lung cancer cell lines.A similar effect was detected on survival upon silencing IKKα/IKKγor inhibiting IKKβ.Conclusions:Exposure of lung cancer cells to IR results in NFκB activation via IKK.The genetic or pharmacological blockage of the RAF1-ERK-IKK-NFκB pathway sensitizes cells to therapeutic doses of radiation.Therefore,the IKK pathway is a promising target for therapeutic intervention in combination with radiotherapy. | Avgi Tsolou Maria Liousia Dimitra Kalamida Stamatia Pouliliou Alexandra Giatromanolaki MichaelKoukourakis | 2017 | Cancer Biology & Medicine2017，14，3： | 2 |
| 2 | Autophagic flux response and glioblastoma sensitivity to radiation显示文摘Objective:Glioblastoma is the most common primary brain tumor in adults and one of the most lethal human tumors.It constitutes a unique non-metastasizing human tumor model with high resistance to radiotherapy and chemotherapy.The current study investigates the association between autophagic flux and glioblastoma cell resistance.Methods:The expression kinetics of autophagy-and lysosome-related proteins following exposure of two glioblastoma cell lines(T98 and U87)to clinically relevant radiation doses was examined.Then,the response of cells resistant to radiotherapy and chemotherapy was investigated after silencing of LC3A,LC3B,and TFEB genes in vitro and in vivo.Results:Following irradiation with 4 Gy,the relatively radioresistant T98 cells exhibited enhanced autophagic flux.The more radiosensitive U87 cell line suffered a blockage of autophagic flux.Silencing of LC3A,LC3B,and TFEB genes in vitro,significantly sensitized cells to radiotherapy and temozolomide(U87:P<0.01 and<0.05,respectively;T98:P<0.01 and<0.01,respectively).Silencing of the LC3A gene sensitized mouse xenografts to radiation.Conclusions:Autophagy in cancer cells may be a key factor of radio-resistance and chemo-resistance in glioblastoma cells.Blocking autophagy may improve the efficacy of radiochemotherapy for glioblastoma patients. | Achilleas G.Mitrakas Dimitra Kalamida Alexandra Giatromanolaki Stamatia Pouliliou Avgi Tsolou Rafail Kyranas Michael I.Koukourakis | 2018 | Cancer Biology & Medicine2018，15，3： | 1 |
| 3 | Construction and characterization of a humanized single chain Fv antibody fragment against the main immunogenic region of the acetylcholine receptor显示文摘 | Avgi Mamalaki | 1999 | Neuroimmunology1999，94，： | 1 |
| 4 | Association of haptoglobin genotype and common cardiovascular risk factors with the amount of iron in atherosclerotic carotid plaques显示文摘 | Christos Lioupis Calypso Barbatis Aggeliki Drougou Vasiliki Koliaraki Avgi Mamalaki Christos Klonaris Sotirios Georgopoulos Vasilios Andrikopoulos Elias Bastounis | 2011 | Atherosclerosis2011，，1： | 1 |
| 5 | Developing a Sustainable Mobility Plan in Piraeus with Special Emphasis on Cycling显示文摘 | Efthimios Bakogiannis Avgi Vassi Maria Siti Georgia Christodoulopoulou | 2016 | Journal of Traffic and Transportation Engineering2016，4，2： | 0 |
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