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| 1 | Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection显示文摘Hepatitis delta virus(HDV) seems to strongly suppress hepatitis B virus(HBV)replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein(HBx) is essential for HBV replication, determination of HBV X gene(HBX)quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5' region between chronic hepatitis delta(CHD) and chronic HBV mono-infected patients.METHODS Twenty-four untreated patients were included: 7/24(29.2%) with HBeAgnegative chronic HBV infection(CI, previously termed inactive carriers), 8/24(33.3%) with HBeAg-negative chronic hepatitis B(CHB) and 9/24(37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels.The HBX 5' region [nucleotides(nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing(MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices(number of haplotypes and number of mutations), abundancebased indices(Hill numbers of order 1 and 2), and functional indices(mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity.RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL,interquartile range(IQR) 3.5-7.9] than CHD(3.4 logIU/mL, IQR 3-7.6)(P = n.s.)or CI(3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statistically significant differences(CHB2.81, IQR 1.11-4.57 vs CHD 8.87, 6.56-11.18, P = 0.038). There were no significant differences in the functional indices, but CI and CHD patients also showed a trend towards greater complexity than CHB. No differences were found for any HBV quasispecies complexity indices between CHD and CI patients. G-to-A and C-to-T nucleotide changes, characteristic of APOBEC3 G, were higher in CHD and CI than in CHB in genotype A haplotypes, but not in genotype D. The proportion of nt G-to-A vs A-to-G changes and C-to-T vs T-to-C changes in genotype A and D haplotypes in CHD patients showed no significant differences. In CHB and CI the results of these comparisons were dependent on HBV genotype.CONCLUSION The lower-replication CHD and CI groups show a trend to higher quasispecies complexity than the higher-replication CHB group. The mechanisms associated with this greater complexity require elucidation. | Cristina Godoy David Tabernero Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Mar?al Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías | 2019 | World Journal of Gastroenterology2019，25，13： | 6 |
| 2 | Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy显示文摘AIM To detect hyper-conserved regions in the hepatitis B virus(HBV) X gene(HBX) 5' region that could be candidates for gene therapy.METHODS The study included 27 chronic hepatitis B treatmentnaive patients in various clinical stages(from chronic infection to cirrhosis and hepatocellular carcinoma, both HBeA g-negative and HBeA g-positive), and infected with HBV genotypes A-F and H. In a serum sample from each patient with viremia > 3.5 log IU/m L, the HBX 5' end region [nucleotide(nt) 1255-1611] was PCRamplified and submitted to next-generation sequencing(NGS). We assessed genotype variants by phylogenetic analysis, and evaluated conservation of this region by calculating the information content of each nucleotide position in a multiple alignment of all unique sequences(haplotypes) obtained by NGS. Conservation at the HBx protein amino acid(aa) level was also analyzed.RESULTS NGS yielded 1333069 sequences from the 27 samples, with a median of 4578 sequences/sample(2487-9279, IQR 2817). In 14/27 patients(51.8%), phylogenetic analysis of viral nucleotide haplotypes showed a complex mixture of genotypic variants. Analysis of the information content in the haplotype multiple alignments detected 2 hyper-conserved nucleotide regions, one in the HBX upstream non-coding region(nt 1255-1286) and the other in the 5' end coding region(nt 1519-1603). This last region coded for a conserved amino acid region(aa 63-76) that partially overlaps a Kunitz-like domain.CONCLUSION Two hyper-conserved regions detected in the HBX 5' end may be of value for targeted gene therapy, regardless of the patients' clinical stage or HBV genotype. | Carolina González David Tabernero Maria Francesca Cortese Josep Gregori Rosario Casillas Mar Riveiro-Barciela Cristina Godoy Sara Sopena Ariadna Rando Marcal Yll Rosa Lopez-Martinez Josep Quer Rafael Esteban Maria Buti Francisco Rodríguez-Frías | 2018 | World Journal of Gastroenterology2018，24，19： | 6 |
| 3 | GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm显示文摘Background: Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination(AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments,which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm(i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction.Results: Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm.However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor(PFE) than in good(GFE) freezability ejaculates in both pre-frozen(1.00 INT·mm^2± 0.14 INT·mm^2 vs.0.72 INT·mm^2± 0.15 INT·mm^2;P < 0.05) and post-thawed(0.96 INT·mm^2± 0.20 INT·mm^2 vs. 70 INT·mm^2± 0.19 INT·mm^2;P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species(ROS) production, and high membrane lipid disorder postthaw(P < 0.05).Conclusions: The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm. | Marc Llavanera Ariadna Delgado-Bermúdez Beatriz Fernandez-Fuertes Sandra Recuero Yentel Mateo Sergi Bonet Isabel Barranco Marc Yeste | 2019 | Journal of Animal Science and Biotechnology2019，10，4： | 4 |
| 4 | Entanglement measures of a new type pseudo-pure state in accelerated frames显示文摘In this work we analyze the characteristics of quantum entangleinent of the Dirac field in noninertial reference frames in the context of a new type pseudo-pure state, which is composed of the Bell states. This will help us to understand the relationship between the relativity and quantum information theory. Some states will be changed from entangled states into separable ones around the critical value F = 1/4, but there is no such a critical value for the variable y related to acceleration a. We find that the negativity Nas (ρ^TA AB 1) increases with F but decreases with the variable y、while the variation of the negativity Nb1b11(ρ^TB1 B1B11)is opposite to that of the negativity N AB1(ρ^TA AB1). We also study the von Neumann entropies S(ρAB1) and S(ρB1B11)、We find that the S(ρAB1) increases with variable y but S(ρB1B11) is independent of it. However, both S(ρAB1) and S(ρB1B11) first decreases with F and then increases with it. The concurrences C(ρAB1) and C(ρB1B11) are also discussed. We find that the former decreases with y while the latter increases with y but both of them first increase with F and then decrease with it. | Qian Dong Ariadna J. Torres-Arenas Guo-Hua Sun Wen-Chao Qiang Shi-Hai Dong | 2019 | Frontiers of physics2019，14，2： | 3 |
| 5 | Hemolymph metabolic variables and immune response in Litopenaeus setiferus adult males : the effect of acclimation显示文摘 | Ariadna Sanchez Crostina Pascual Adolfo Sánchez | 2001 | Aquaculture2001，198，： | 1 |
| 6 | 显示文摘 | Ariadna A Morales Heriberto Pfeiffer Arturo Delfin | 2001 | Materials Letter2001，50，： | 1 |
| 7 | Procyanidin fractions from pine (Pinus pinaster) bark: radical scavenging power in solution, antioxidant activity in emulsion, and antiproliferative effect in melanoma cells 显示文摘 | Tourino Sonia Selga Ariadna Jimenez Aurora | 2005 | Journal of agricultural and food chemistry2005，53，12： | 1 |
| 8 | Detection of Theileria equi and Babesia caballi infections in Vene- zuelan horses using competitive-inhibition ELISA and PCR显示文摘 | Romel R Ariadna R R America E | 2013 | Veterinary Parasitology2013，226，： | 1 |
| 9 | Relation between reproductive, physiological, and immunological condition of Litopenaeus setiferus pre-adult males fed different dietary protein levels ( Crustaeea; Penaeidae)显示文摘 | Yannick G Ariadna S Gabriela G | 2006 | Animal Reproduction Science2006，92，： | 1 |
| 10 | Control of photoperiod-regulated tuberization in potato by the Arabidopsis floweringtime gene CONSTANS显示文摘 | Jaime F Mart' nez-Garc' a Ariadna Virgo's-Soler | 2002 | PNAS2002，99，15： | 1 |
| 11 | Cryptosporidium molnari n. sp. (Apicomplexa: Cryptosporidiidae) infecting two marine fish species, Sparus aurata L. and Dicentrarchus labrax L.显示文摘 | Pilar Alvarez-Pellitero Ariadna Sitjà-Bobadilla | 2002 | International Journal for Parasitology2002，，8： | 1 |
| 12 | Paradoxical effects of cytokines in tumor immune surveillance and tumor immune escape显示文摘 | Flavio Salazar-Onfray Mercedes N. López Ariadna Mendoza-Naranjo | 2007 | Cytokine and Growth Factor Reviews2007，，1： | 1 |
| 13 | Lentivirus vector can be readministered to nasal epithelia without blocking immune responses显示文摘 | Patrick L S Ariadna C A Kim A B | 2008 | JVirol2008，82，10： | 1 |
| 14 | Growth hormone as an invitro phagocyte-activating factor in the gilthead sea \)Team(Sparusaurata)显示文摘 | Josep A C G Ariadna S B Pilar A P | 1997 | Cell Tissue Res1997，287，： | 1 |
| 15 | Tetrapartite entanglem ent features of W-C lass state in uniform acceleration显示文摘Using the single-mode approximatiou,we first calculate entanglement measures such as negativity(1-3 and 11 tangles)and von Neumann entropy for a tetrapartite W-Class system in noninertial fiarne and then anal^e the whole entanglement measures,the residualπ4 and geometricП4 average of tangles.Notice that the difference betweenπ4 andП4 is very small or disappears with the increasing accelerated observers.The entanglement properties are compared among the different cases from oue accelerated observer to four accelerated observers.The results show that there still exists entanglement for the complete system even when accelemtion r tends to infinity.The degree of entanglement is disappeared for the 1-1 tangle case when the acceleration r>0.472473.We reexamine the Unriih effect in noninertiai frames.It is shown that the entanglement system in which only one qubit is accelerated is more robust than those eutangled systems in whicli two or three or four qubits are accelerated.It is also found that the von Neumann entropy S of the total system always increases with the increasing accelerated observers,but the Sκξand Sκζδwith two and three involved noniuertial qubits first increases and then decreases with the acceleration parameter r,but they are equal to constants 1 and 0.811278 respectively for zero involved iioniiiertial qubit. | Qian Dong Ariadna J.Torres-Arenas Guo-Hua Sun Shi-Hai Dong | 2020 | Frontiers of physics2020，15，1： | 1 |
| 16 | Correction of high myopia with different phakic anterior chamber intraocular lenses: ICARE angle-supported lens and Verisyse iris-claw lens显示文摘 | Stanislawa Gierek-Ciaciura Ariadna Gierek-Lapinska Krzysztof Ochalik Ewa Mrukwa-Kominek | 2007 | Graefe’s Archive for Clinical and Experimental Ophthalmology2007，，1： | 1 |
| 17 | Transcriptionalregulation of the rice arginine decarboxylase (Adel) andS-adenosylmethionine decarboxylase (SAMDC) genes by methyljasmonate 显示文摘 | Ariadna P Ludovic B Yuan D | 2010 | Plant Physiol Biochem2010，48，： | 1 |
| 18 | Autotrophic nitrite removal in the cathode of microbial fuel cells显示文摘 | Puig Sebastia Serra Marc Vilar-Sanz Ariadna | 2013 | Bioresource Technology2013，47，5： | 1 |
| 19 | Ethnic differences in complementary and alternative medicine use among patients with diabetes显示文摘 | Leonel Villa-Caballero Candis M. Morello Megan E. Chynoweth Ariadna Prieto-Rosinol William H. Polonsky Lawrence A. Palinkas Steven V. Edelman | 2010 | Complementary Therapies in Medicine2010，，6： | 1 |
| 20 | Liquidity and optimal market transpareney显示文摘 | Ariadna Dumitreseu | 2010 | European Financial Management2010，16，： | 1 |
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