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		题名	作者	年代	出处	被引量
	1	Transcriptomics-based identification and characterization of 11 CYP450 genes of Panax ginseng responsive to MeJA显示文摘Cytochromes P450 (CYP450s),a superfamily of mono-oxygenases,are essential to generate highly functionalized secondary metabolites in plants and contribute to the diversification of specialized triterpenoid biosynthesis in eudicots.However,screening and identifying the exact CYP450 genes in ginsenoside biosynthesis is extremely challenging due to existence of large quantities of members in CYP450 superfamily.Therefore,to screen the CYP450 genes involved in ginsenoside bio- synthesis,transcriptome dataset of Panax ginseng was created in our previous work using the technique of the next-generation sequencing.On the basis of bioinformatics analysis,16 putative CYP450 genes with significant differential expression were screened from the dataset and submit- ted to GenBank,in which 11 of them have been cloned.Methyl jasmonate (MeJA)was used as an elicitor to analyze the expression profiles of candidate CYP450 genes by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR).The results of qRT-PCR analysis revealed that the expression of some CYP450 genes were strongly induced by MeJA and showed different transcription levels at different treatment time points.Homology analysis indicated that each putative CYP450 protein of P.ginseng has a conserved domain consisting of E-E-R-F-P-R-G. The CYP450 genes were screened and cloned here to enrich the resources of CYP450 genes,and the results of bioinformatics analysis provided a foundation to further identify the function of CYP450s involved in ginsenoside biosynthesis.Furthermore,this study facilitated the construction of microbial cell factories for increasing the production of ginsenosides by means of metabolic engineering.	Xu Zeng Tiao Luo Jijia Li Gui Li Donghua Zhou Tuo Liu Xian Zou Aparna Pandey Zhiyong Luo	2018	Acta Biochimica et Biophysica Sinica2018，50，11：	2
	2	Neuron Specific Enolase and C-reactive Protein Levels in Stroke and Its Subtypes: Correlation with Degree of Disability显示文摘	Aparna Pandey Amit Kumar Shrivastava Kiran Saxena	2014	Neurochemical Research2014，，8：	1

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