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| 1 | An alternative,zeaxanthin epoxidase-independent abscisic acid biosynthetic pathway in plants显示文摘Abscisic acid(ABA)is an important carotenoid-derived phytohormone that plays essential roles in plant response to biotic and abiotic stresses as well as in various physiological and developmental processes.In Arabidopsis,ABA biosynthesis starts with the epoxidation of zeaxanthin by the ABA DEFICIENT 1(ABA1)enzyme,leading to epoxycarotenoids;e.g.,violaxanthin.The oxidative cleavage of 9-cis-epoxycaro-tenoids,a key regulatory step catalyzed by 9-C/S-EPOXYCAROTENOID DIOXYGENASE,forms xanthoxin,which is converted in further rea.ctions mediated by ABA DEFICIENT 2(ABA2),ABA DEFICIENT 3(ABA3),and ABSCISIC ALDEHYDE OXIDASE 3(AAO3)into ABA.By combining genetic and biochemical approaches,we unravel here an ABA1-independent ABA biosynthetic pathway starting upstream of zeaxanthin.We iden-tified the carotenoid cleavage products(i.e.,apocarotenoids,β-apo-11-carotenal,9-cis-β-apo-11-carotenal,3-OH-β-apo-11-carotenal,and 9-cis-3-OH-β-apo-11-carotenal)as intermediates of this ABA1-independent ABA biosynthetic pathway.Using labeled compounds,we showed thatβ-apo-11-carotenal,9-cis-β-apo-11-carotenal,and 3-OH-β-apo-11-carotenal are successively converted into 9-cis-3-OH-β-apo-11-carotenal,xanthoxin,and finally into ABA in both Arabidopsis and rice.When applied to Arabidopsis,theseβ-apo-11-carotenoids exert ABA biological functions,such as maintaining seed dormancy and inducing the expression of ABA-responsive genes.Moreover,the transcdptomic analysis revealed a high overlap of differentially expressed genes regulated byβ-apo-11-carotenoids and ABA,suggesting thatβ-apo-11-carot-enoids exert ABA-independent regulatory activities.Taken together,our study identifies a biological function for the common plant metabolites,β-apo-11-carotenoids,extends our knowledge about ABA biosynthesis,and provides new insights into plant apocarotenoid metabolic networks. | Kun-Peng Jia Jianing Mi Shawkat Ali Hajime Ohyanagi Juan C.Moreno Abdugaffor Ablazov Aparna Balakrishna Lamis Berqdar Alessia Fiore Gianfranco Diretto Claudio Martínez Angel R.de Lera Takashi Gojobori Salim Al-Babili | 2022 | Molecular Plant2022，15，1： | 2 |
| 2 | 3-Hydroxycarlactone, a Novel Product of the Strigolactone Biosynthesis Core Pathway显示文摘 | Lina Baz Narumi Mori Jianinq Mi Muhammad Jamil Boubacar A. Kountche Xiujie Guo Aparna Balakrishna Kun-Peng Jia Martina Vermathen Kohki Akiyama Salim AI-Babili | 2018 | Molecular Plant2018，11，10： | 1 |
| 3 | Installing the neurospora carotenoid pathway in plants enables cytosolic formation of provitamin A and its sequestration in lipid droplets显示文摘Vitamin A deficiency remains a severe global health issue,which creates a need to biofortify crops with provitamin A carotenoids(PACs).Expanding plant cell capacity for synthesis and storing of PACs outside the plastids is a promising biofortification strategy that has been little explored.Here,we engineered PAC formation and sequestration in the cytosol of Nicotiana benthamiana leaves,Arabidopsis seeds,and citrus callus cells,using a fungal(Neurospora crassa)carotenoid pathway that consists of only three enzymes converting C5 isopentenyl building blocks formed from mevalonic acid into PACs,including β-carotene.This strategy led to the accumulation of significant amounts of phytoene and γ-and β-carotene,in addition to fungal,health-promoting carotenes with 13 conjugated double bonds,such as the PAC torulene,in the cytosol.Increasing the isopentenyl diphosphate pool by adding a truncated Arabidopsis hydroxymethylglutaryl-coenzyme A reductase substantially increased cytosolic carotene production.Engineered carotenes accumulate in cytosolic lipid droplets(CLDs),which represent a novel sequestering sink for storing these pigments in plant cytosol.Importantly,β-carotene accumulated in the cytosol of citrus callus cells was more light stable compared to compared with plastidialβ-carotene.Moreover,engineering cytosolic carotene formation increased the number of large-sized CLDs and the levels of β-apocarotenoids,including retinal,the aldehyde corresponding to vitamin A.Collectively,our study opens up the possibility of exploiting the high-flux mevalonic acid pathway for PAC biosynthesis and enhancing carotenoid sink capacity in green and non-green plant tissues,especially in lipid-storing seeds,and thus paves the way for further optimization of carotenoid biofortification in crops. | Xiongjie Zheng Yasha Zhang Aparna Balakrishna Kit Xi Liew Hendrik N.J.Kuijer Ting Ting Xiao Ikram Blilou Salim Al-Babili | 2023 | Molecular Plant2023，16，6： | 0 |
| 4 | Screening of potential aquatic probiotics from the major microflora of guppies (Poecilia reticulata)显示文摘鱼(Poecilia reticulata ) 为 probiotics 被用作来源。46 细菌孤立从 guppy 的皮肤,鳃,勇气和肠被获得, Poecilia reticulata (从在 Kottayam 的一个政府模型鱼农场镇定,印度) 。上述孤立的紧张,四孤立对五指示物紧张基于他们的禁止的光谱被选择, Aeromonas hydrophila 1739, Vibrio cholerae 3906,黄质菌属 2495, Acinetobacter 1271 和 Alcaligenes 1424 (从微生物引起的类型文化收集(MTCC ) Chandigarh 收集的标准文化,印度) 。在结果之中孤立,二是克积极的球菌,也就是 MBTU-PB2 和 MBTU-PB3 并且属于类葡萄球菌。另外的二是克否定的杆,也就是 MBTU-PB1 和 MBTU-PB4 类 Enterobacter 和 Acinetobacter,分别地。如同禁止的物质(BLIS ) ,抗菌素敏感和生长侧面也被决定,这些的基本 probiotic 特征孤立例如 bacteriocin 的生产。上述四孤立的紧张比五指示物紧张展出了不同对抗。在孵化期间,抗菌剂活动逐渐地在抑制地区增加了并且被 lag 时期影响() 并且双倍工资。为大多数四选择紧张的 lag 时期比指示物紧张的那些缺少, isolates 有不同生长率() 比指示物紧张。然而,所有四孤立生产 BLIS 紧张对指示物紧张举办了不同 BLIS 活动。抵销的房间的处理免费上层清液选择与消除或减少 BLIS 活动的朊酶孤立,建议禁止的混合物的一个 proteinaceous 性质。进一步,最佳 BLIS 活动在孵化的 18 h 以后在中立 pH 被观察。抗菌素敏感试金表明 isolates 产生习惯性地使用的抗菌素,而 plasmid 侧面证明 plasmids 没在四孤立的紧张的对抗性质有角色。结果证明 isolates 能是为在水产业的 biocontrol 代理人的有希望的来源。 | Aparna BALAKRISHNA T.R.KEERTHI | 2012 | Frontiers of Chemical Science and Engineering2012，6，2： | 0 |
| 5 | 3-Hydroxycarlactone, a Novel Product of theStrigolactone Biosynthesis Core Pathway显示文摘(Molecular Plant11(10):1312-1314;October 2018;http://gffzzd3cc09b8251d45dfso6nobnfcuvbo6k9n.ffgz.tsg.suse.edu.cn/10.1016/j.molp.2018.06.008)The amounts of CL and 3-H-CL shown in the original Figure 1D and Supplementary Figure 13 are inaccurate,due to an erroneous prep-aration and mixing up of samples.In addition,there is an error in the designation of the spectra of two peaks in the original Figure 1A,inwhich the spectrum designated as I should belong to peak II,andvice versa.We have repeated the related experiments and quanti-fication,and the corrected Figure 1 and Supplementary Figure 13 are shown below. | Lina Baz Narumi Mori Jianing Mi Muhammad Jamil Boubacar A.Kountche Xiujie Guo Aparna Balakrishna Kun-Peng Jia Martina Vermathen Kohki Akiyama Salim Al-Babili | 2021 | Molecular Plant2021，14，10： | 0 |
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