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| 1 | Transcriptome analysis of Sinorhizobium meliloti nodule bacteria in nifA mutant background显示文摘Gene expression profiles of a Si- norhizobium meliloti 1021 nifA mutant and wild type nodule bacteria were compared using whole genome microarrays. The results revealed a large scale al- teration of gene expression (601 genes) in the nifA minus background. The loss of NifA altered the ex- pression of many functional groups of genes (mac- romolecular metabolism, TCA cycle and respiration, nodulation and nitrogen fixation) and may lead to quite different life stages of the nodule bacteria. Upregulation of fixK and its associated genes was observed in the nifA mutant nodule bacteria. Addi- tional quantitative real-time PCR experiments re- vealed that the transcript levels of fixLJ were signifi- cantly upshifted in the nifA mutant nodule bacteria. Putative NifA binding sites were predicted by a statis- tical method in the upstream sequences of 13 differ- entially regulated genes from the nifA- transcriptome. | TIAN Zhexian ZOU Huasong LI Jian ZHANG Yuantao LIU Ying YU Guanqiao ZHU Jiabi RUBERG Silvia BECKER Anke WANG Yiping | 2006 | Chinese Science Bulletin2006，51，17： | 4 |
| 2 | 异源nifA基因对苜蓿中华根瘤菌nifA突变体的互补分析显示文摘为深入研究苜蓿中华根瘤菌NifA的特性,分别用组成型表达的慢生型大豆根瘤菌和紫云英根瘤菌的nifA基因互补苜蓿中华根瘤菌nifA突变体,观察其共生表型.结果表明,慢生型大豆根瘤菌和紫云英根瘤菌nifA基因不能互补苜蓿中华根瘤菌nifA突变体的共生表型.以苜蓿中华根瘤菌nifA突变体为遗传背景,利用全基因组微阵列实验比较分析引入异源nifA基因后产生的基因表达谱的变化.结果显示,苜蓿中华根瘤菌自身NifA蛋白引起238个基因的表达发生变化.这些表达差异的基因分属共生、能量和中心代谢、持家、细胞结构与运输等生物学功能组.慢生型大豆根瘤菌、紫云英根瘤菌和阴沟肠杆菌的NifA蛋白分别引起了20,7和9个基因的表达发生变化.这些基因主要是固氮相关基因,但差异不及苜蓿中华根瘤菌NifA互补菌明显.以苜蓿中华根瘤菌nifH启动子与lacZ融合基因为报道基因,研究nifH的表达.结果指出,慢生型大豆根瘤菌和紫云英根瘤菌的NifA蛋白只能部分激活苜蓿中华根瘤菌nifH的表达,激活水平分别为苜蓿中华根瘤菌NifA蛋白激活率的70%和50%,与微阵列实验结果相符. | 姚振华 田哲贤 戴小密 Anke Becker 李健 严海芹 肖琰 朱家璧 俞冠翘 Silvia Rüberg 王忆平 邹华松 | 2006 | 科学通报2006，51，19： | 4 |
| 3 | nifA突变的苜蓿根瘤菌在根瘤中的转录组学分析显示文摘用全基因组微阵列比较了根瘤中苜蓿根瘤菌1021的nifA突变菌和野生菌的基因表达谱.分析结果表明,nifA的缺失引起601个基因的表达发生变化.这些基因分别属于生物大分子的合成代谢、三羧酸循环及呼吸代谢以及结瘤固氮过程等多个功能组,预示着根瘤中细菌的生长状态发生了显著的变化.在根瘤中,fixK以及受其激活的基因在nifA突变菌中的表达量显著高于野生菌.定量实时PCR分析表明,根瘤中fixLJ的转录水平在nifA突变菌中显著高于野生菌.通过统计学方法从13个表达发生显著变化的基因的上游调控序列中找到推测的NifA结合位点. | 田哲贤 邹华松 李健 张远涛 刘影 俞冠翘 朱家璧 Silvia Rüberg Anke Becker 王忆平 | 2006 | 科学通报2006，51，15： | 3 |
| 4 | Complementation analyses of Sinorhizobium meliloti nifA mutant with different originated nifA genes显示文摘推断的一个以前的工作 Enterobacter 下水道的 nifA 基因没恢复 Sinorhizobium meliloti nifA 的共生显型变异。在现在的学习, Bra-dyrhizobium 的 twonifA 基因日本嗯并且 Mesorhizobium huakuii 也没恢复 S 的共生显型。meliloti nifA 异种。在微数组试验的整个 genomic, 238 基因被发现是在 S 以后表示的差别。meliloti nifA 组成地在它的 nifAmutant 被表示了。相反,当表示 B.japonium 时,仅仅 20, 7 和 9 基因改变了他们的 transcriptional 层次, M。在 Sm nifA 异种的 huakuii 和 Enterobacter 下水道 nifA 基因独立。这些基因包括房子保留,精力和中央中间代谢被分类进几官能基,运输系统和共生。有趣地, nifHoperons,显示出的高表示面对任何一个 B 铺平的基因。japonium 或 M。huakuii NifA,它被随后的紫胶 Z 熔化实验证实。 | YAO Zhenhua TIAN Zhexian DAI Xiaomi BECKER Anke LI Jian YAN Haiqin XIAO Yan ZHU Jiabi YU Guanqiao RUVERG Silvia WANG Yiping ZOU Huasong | 2006 | Chinese Science Bulletin2006，51，22： | 2 |
| 5 | 苜蓿根瘤菌1021外源cAMP上调glnⅡ及glnK-amtB的表达显示文摘苜蓿根瘤菌1021中多个腺苷酸环化酶基因的存在,暗示着cAMP的重要性.本研究用全基因组DNA微阵列分析了外源cAMP对苜蓿根瘤菌的功能.分析结果表明,glnⅡ和glnK的转录水平在外源cAMP存在时有明显上调.通过测定glnⅡ和glnK启动子与lacZYA报告基因融合系统在苜蓿根瘤菌体内的活性,再次证实外源cAMP对glnⅡ和glnK启动子表达有激活作用. | 田哲贤 毛贤军 苏玮 李健 Anke Becker 王忆平 | 2006 | 科学通报2006，51，14： | 1 |
| 6 | Two new bioactive diterpenes from Lepista sordida显示文摘 | Mazur X Becker U Anke T | 1996 | Phytochemistry1996，43，2： | 1 |
| 7 | Two new bioactive diterpenefrom Lepista sordida显示文摘 | MZUR X BECKER U ANKE T | 1996 | Phytochemistry1996，43，2： | 1 |
| 8 | Exogenous cAMP upregulates the expression of glnII and glnK-amtB genes in Sinorhizobium meliloti 1021显示文摘The existence of multiple adenylate cy- clase encoding genes implies the importance of cAMP in Sinorhizobium meliloti 1021. In this study, as a pioneer step of understanding cAMP roles, mi- croarray analysis on S. meliloti was carried out for the function of exogenous cAMP. To our surprise, the result showed that the transcriptions of glnII and glnK genes were significantly upshifted in the presence of exogenous cAMP in S. meliloti. This phenomenon is further confirmed in S. meliloti that the expression of either glnII or glnK promoter-lacZ translational fusion is higher in the presence of exogenous cAMP. Therefore, for the first time, we have identified genes from S. meliloti whose expression is activated by cAMP. The potential physiological role of upregula- tion of glnII and glnK by cAMP is discussed. | TIAN Zhexian MAO Xianjun SU Wei LI Jian BECKER Anke WANG Yiping | 2006 | Chinese Science Bulletin2006，51，16： | 1 |
| 9 | Two new bioactive diterpenes from Lepista sordida 显示文摘 | Mazur X Becker U Anke T | 1996 | Phytochemistry1996，43，2： | 1 |
| 10 | Oudemansin,strobilurin A,strobilurin B and myxothiazol:new inhibitors of the Bcl segment of the respiratory chain with an E-Methoxyacrylate system as common structural element显示文摘 | Becker W F Jagow G V Anke T | 1981 | FEBS Lett1981，132，： | 1 |
| 11 | Two new bioactive diterpenes from Lepista sordida显示文摘 | becker U Anke T | 1996 | Phytochemistry1996，43，2： | 1 |
| 12 | Two new bioactive diterpenes from Lepista sordida显示文摘 | Mazur X Becker U Anke T | 1996 | Phytochemistry1996，43，2： | 1 |
| 13 | Two new bioactive diterpenes from Lepista sordida显示文摘 | Mazur X becker U Anke T | 1996 | Phytochemistry1996，43，2： | 1 |
| 14 | What can bacterial genome research teach us about bacteria–plant interactions?显示文摘 | Alfred Pühler Matthieu Arlat Anke Becker Michael G?ttfert John P Morrissey Fergal O’Gara | 2004 | Current Opinion in Plant Biology2004，，2： | 1 |
| 15 | Oudemansin,Strobilurin A, Strobilurin B and Myxothiazol: New Inhibitors of thebcl Segment of the Respiratory Chain with an E-TS-MethoxyacrylateSystem as Common Structural Element显示文摘 | BECKER W F VON JAGOW G ANKE T | 1981 | FEBS Letters1981，132，： | 1 |
| 16 | Development of bioinformatic tools to support EST-sequencing,In silico-and micro array-based transcriptome profiling in Mycorrhizal symbioses显示文摘 | Helge Kuster Anke Becker Christian Firnhaber | 2007 | Science Direct2007，，68： | 1 |
| 17 | Oudemansin, Strobilurin A, Strobilurin B and Myxothiazol: New Inhibitors of the Bcl Segment of the Respiratory Chain with an E-β- Methoxyacrylate System as Common Structural Element 显示文摘 | BECKER W F JAGOW G V ANKE T | 1981 | FEBS Lett1981，132，： | 1 |
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