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| 1 | Anti-tumor necrosis factor α therapy associates to type 17 helper T lymphocytes immunological shift and significant microbial changes in dextran sodium sulphate colitis显示文摘BACKGROUND Anti-tumor necrosis factor α(TNFα) represents the best therapeutic option to induce mucosal healing and clinical remission in patients with moderate-severe ulcerative colitis. On the other side gut microbiota plays a crucial role in pathogenesis of ulcerative colitis but few information exists on how microbiota changes following anti-TNFα therapy and on microbiota role in mucosal healing.AIM To elucidate whether gut microbiota and immune system changes appear following anti TNFα therapy during dextran sulfate sodium(DSS) colitis.METHODS Eighty C57 BL/6 mice were divided into four groups: 'No DSS', 'No DSS + antiTNFα', 'DSS' and 'DSS + anti-TNFα'. 'DSS' and 'DSS + anti-TNFα' were treated for 5 d with 3% DSS. At day 3, mice whithin 'No DSS+anti-TNFα' and'DSS+anti-TNFα' group received 5 mg/kg of an anti-TNFα agent. Forty mice were sacrificed at day 5, forty at day 12, after one week of recovery post DSS. The severity of colitis was assessed by a clinical score(Disease Activity Index), colon length and histology. Bacteria such as Bacteroides, Clostridiaceae, Enterococcaceae and Fecalibacterium prausnitzii(F. prausnitzii) were evaluated by quantitative PCR.Type 1 helper T lymphocytes(Th1), type 17 helper T lymphocytes(Th17) and CD4+ regulatory T lymphocytes(Treg) distributions in the mesenteric lymph node(MLN) were studied by flow cytometry.RESULTS Bacteria associated with a healthy state(i.e., such as Bacteroides, Clostridiaceae and F. prausnitzii) decreased during colitis and increased in course of anti-TNFαtreatment. Conversely, microorganisms belonging to Enterococcaceae genera,which are linked to inflammatory processes, showed an opposite trend.Furthermore, in colitic mice treated with anti-TNFα microbial changes were associated with an initial increase(day 5 of the colitis) in Treg cells and a consequent decrease(day 12 post DSS) in Th1 and Th17 frequency cells. Healthy mice treated with anti-TNFα showed the same histological, microbial and immune features of untreated colitic mice. 'No DSS + anti-TNFα' group showed a lymphomononuclear infiltrate both at 5 th and 12 th d at hematoxylin and eosin staining, an increase of in Th1 and Th17 frequency at day 12, an increase of Enterococcaceae at day 5, a decrease of Bacteroides and Clostridiaceae at day 12.CONCLUSION Anti-TNFα treatment in experimental model of colitis improves disease activity but it is associated to an increase in Th17 pathway together with gut microbiota alteration. | Valentina Petito Cristina Graziani Loris R Lopetuso Marco Fossati Alessandra Battaglia Vincenzo Arena Domenico Scannone Gianluca Quaranta Andrea Quagliariello Federica Del Chierico Lorenza Putignani Luca Masucci Maurizio Sanguinetti Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2019 | World Journal of Gastroenterology2019，25，12： | 2 |
| 2 | Slructural Response of Grid-Reinforced Bituminous Pavement显示文摘 | Andrea Graziani Emiliano Pasquini Gilda Ferrolti | 2014 | Materials and Struclures2014，47，8： | 1 |
| 3 | The Humoral Pattern Recognition Molecule PTX3 Is a Key Component of Innate Immunity against Urinary Tract Infection显示文摘 | Sébastien Jaillon Federica Moalli Bryndis Ragnarsdottir Eduardo Bonavita Manoj Puthia Federica Riva Elisa Barbati Manuela Nebuloni Lidija Cvetko Krajinovic Alemka Markotic Sonia Valentino Andrea Doni Silvia Tartari Giorgio Graziani Alessandro Montanelli Y | 2014 | Immunity2014，，4： | 1 |
| 4 | Failure of aldosterone suppression despite angiotensin-converting enzyme (ACE) inhibitor administration in chronic heart failure is associated with ACE DD genotype显示文摘 | Mariantonietta Cicoira Luisa Zanolla Andrea Rossi Giorgio Golia Lorenzo Franceschini Giulio Cabrini Alberto Bonizzato Maristella Graziani Stefan D Anker Andrew J.S Coats Piero Zardini | 2001 | Journal of the American College of Cardiology2001，，7： | 1 |
| 5 | Experimental characterization of the 3D linear viscoelastic behavior of cold recycled bitumen emulsion mixtures显示文摘Cold mixtures with bitumen emulsion are produced at ambient temperature, leading to substantial reductions of energy consumption and atmospheric emissions. In cold recycling applications, cement is normally used to improve the mixture performance. Thus, the rheological behavior of cold recycled mixtures is different from that of conventional hot mixtures because it is due to the interaction of fresh bitumen, aged bitumen and cementitious bonds. In this study, we investigated the three-dimensional(3 D) linear viscoelastic(LVE) behavior of a cement-bitumen treated material(CBTM) mixture fabricated using bitumen emulsion and cement. For comparison, we also investigated the 3 D LVE behavior of hot-mix asphalt containing 25% of reclaimed asphalt and fabricated using polymermodified binder. Sinusoidal axial tests on cylindrical specimens, were carried out at various temperatures(from 0℃ to 50℃) and frequencies(from 0.1 to 12 Hz). The complex Young’s modulus E~*and the complex Poisson’s ratio v~*were determined through the measurement of axial and transverse strain. We show that when considering E*, CBTM mixtures may be considered thermo-rheologically simple and the Huet-Sayegh model can be used to simulate the frequencyetemperature dependence. On the other hand, when considering v*the behavior of CBTM mixtures is very different from that of hot mix asphalt. In particular, its absolute value is almost constant and very close to 0.15. | Andrea Graziani Carlotta Godenzoni Francesco Canestrari | 2019 | Journal of Traffic and Transportation Engineering(English Edition)2019，6，4： | 1 |
| 6 | Alterations of negative regulators of cytokine signalling in immunodeficiency‐related non‐Hodgkin lymphoma显示文摘 | Daniela Capello Annunziata Gloghini Gianluca Baldanzi Maurizio Martini Clara Deambrogi Marco Lucioni Daniela Piranda Rosella Famà Andrea Graziani Michele Spina Umberto Tirelli Marco Paulli Luigi Maria Larocca Gianluca Gaidano Antonino Carbone Fabiola Sini | 2012 | Hematol Oncol2012，，1： | 1 |
| 7 | Infliximab does not increase colonic cancer risk associated to murine chronic colitis显示文摘AIM To explore the influence of Infliximab(IFX) on cancer progression in a murine model of colonic cancer associated to chronic colitis.METHODS AOM/DSS model was induced in C57BL/6 mice. Mice were injected with IFX(5 mg/kg) during each DSS cycle while control mice received saline. Body weight, occult blood test and stool consistency were measured to calculate the disease activity index(DAI). Mice were sacrificed at week 10 and colons were analyzed macroscopically and microscopically for number of cancers and degree of inflammation. MTT assay was performed on CT26 to evaluate the potential IFX role on metabolic activity and proliferation. Cells were incubated with TNF-α or IFX or TNF-α plus IFX, and cell vitality was evaluated after 6, 24 and 48 h. The same setting was used after pre-incubation with TNF-α for 24 h.RESULTS IFX significantly reduced DAI and body weight loss in mice compared with controls, preserving also colon length at sacrifice. Histological score was also reduced in treated mice. At macroscopic analysis, IFX treated mice showed a lower number of tumor lesions compared to controls. This was confirmed at microscopic analysis, although differences were not statistically significant. In vitro, IFX treated CT26 maintained similar proliferation ability at MTT test, both when exposed to IFX alone and when associated to TNF-α.CONCLUSION IFX did not increase colonic cancer risk in AOM-DSS model of cancer on chronic colitis nor influence directly the proliferation of murine colon cancer epithelial cells. | Loris R Lopetuso Valentina Petito Tiziano Zinicola Cristina Graziani Viviana Gerardi Vincenzo Arena Maria Emiliana Caristo Andrea Poscia Giovanni Cammarota Alfredo Papa Valerio Cufino Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2016 | World Journal of Gastroenterology2016，22，44： | 0 |
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