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| 1 | Cryopreservation for delayed circulating tumor cell isolation is a valid strategy for prognostic association of circulating tumor cells in gastroesophageal cancer显示文摘AIM To demonstrate the feasibility of cryopreservation of peripheral blood mononuclear cells(PBMCs) for prognostic circulating tumor cell(CTC) detection in gastroesophageal cancer.METHODS Using 7.5 m L blood samples collected in EDTA tubes from patients with gastroesopheagal adenocarcinoma, CTCs were isolated by epithelial cell adhesion molecule based immunomagnetic capture using the Iso Flux platform. Paired specimens taken during the same blood draw(n = 15) were used to compare number of CTCs isolated from fresh and cryopreserved PBMCs. Blood samples were processed within 24 h to recover the PBMC fraction, with PBMCs used for fresh analysis immediately processed for CTC isolation. Cryopreservation of PBMCs lasted from 2 wk to 25.2 mo(median 14.6 mo). CTCs isolated from pre-treatment cryopreserved PBMCs(n = 43) were examined for associations with clinicopathological variables and survival outcomes.RESULTS While there was a significant trend to a decrease in CTC numbers associated with cryopreserved specimens(mean number of CTCs 34.4 vs 51.5, P = 0.04), this was predominately in samples with a total CTC count of > 50, with low CTC count samples less affected(P = 0.06). There was no significant association between the duration of cryopreservation and number of CTCs. In cryopreserved PBMCs from patient samples prior to treatment, a high CTC count(> 17) was associated with poorer overall survival(OS)(n = 43, HR = 4.4, 95%CI: 1.7-11.7, P = 0.0013). In multivariate analysis, after controlling for sex, age, stage, ECOG performance status, and primary tumor location, a high CTC count remained significantly associated with a poorer OS(HR = 3.7, 95%CI: 1.2-12.4, P = 0.03). CONCLUSION PBMC cryopreservation for delayed CTC isolation is a valid strategy to assist with sample collection, transporting and processing. | Daniel Brungs David Lynch Alison WS Luk Elahe Minaei Marie Ranson Morteza Aghmesheh Kara L Vine Martin Carolan Mouhannad Jaber Paul de Souza Therese M Becker | 2018 | World Journal of Gastroenterology2018，24，7： | 5 |
| 2 | International physical activity questionnaire: 12-country reliability and validity显示文摘 | CORA L CRAING ALISON L SJOSTROM M | 2003 | Med Sci Sports Exerc2003，35，8： | 1 |
| 3 | Cancer stem cells:problems for therapy显示文摘 | Alison M R Lira S M Nieholson L J | 2011 | JPathol2011，223，2： | 1 |
| 4 | Tolerance of Antarctic cyanobacterial mats to enhanced UV radiation显示文摘 | ALISON L G ALISTAIR W M PEDRO O M | 2001 | FEMS Microbiology Ecology2001，37，： | 1 |
| 5 | The new stem cell biology:something for every显示文摘 | Preston S L Alison M R Forbes S J | 2003 | J Clin Pathol:Mol Pathol2003，56，： | 1 |
| 6 | Influence of grain morphology on electron transport in dye sensitized nanocrystalline solar cells 显示文摘 | Cass M J Qiu F L Alison W B | 2003 | J Phys Chem B2003，107，： | 1 |
| 7 | Cancer stem cells: in the line of fire显示文摘 | Alison M R Lin W R Lira S M L | 2012 | Cancer Treat Rev2012，38，6： | 1 |
| 8 | A low-temperature-responsive translation elongation factor let from barley显示文摘 | M Alison Dunn Alison Morris Peter L | 1991 | Mol Gen Genet1991，229，： | 1 |
| 9 | Highly active iron and cobalt catalysts for the polymerization of ethylene 显示文摘 | Brooke L Small Maurice Brookhart Alison M A Bennett | 1998 | J Am Chem Soc1998，120，16： | 1 |
| 10 | The new stem cell biology : something for everyone显示文摘 | PRESTON S L ALISON M R FORBES S J | 2003 | Mol Pathol2003，56，2： | 1 |
| 11 | The new stem cell biology: something for everyone 显示文摘 | Preston S L Alison M R Forbes S J | 2003 | J Clin Pathol:Mol pathol2003，56，： | 1 |
| 12 | The increasing prevalence of autism spec trum disorders显示文摘 | Johnny L Alison M | 2011 | Res Autism Spect Dis2011，5，5： | 1 |
| 13 | The new stem cell biology: something for everyone 显示文摘 | PRESTO S L ALISON M R FORBES S J | 2003 | Mol Pathol2003，56，： | 1 |
| 14 | Tolerance of Antarctic cyanobacterial mats to enhanced UV radiation显示文摘 | Alison L G Alistair W M Pedro O M | 2001 | FEMS Microbiology Ecology2001，37，： | 1 |
| 15 | Seasonal variation and the immune response : a fish perspective 显示文摘 | Tim J B Kim D T Alison L M | 2007 | Fish Shellfish Immu- nol2007，22，： | 1 |
| 16 | Diversity of the ring-cleaving dloxygenase gene pcaH in a salt marsh bacterial community 显示文摘 | Alison B Elien L N Mary A M | 2001 | Applied and Environmental Microbiology2001，67，12： | 1 |
| 17 | Computation of cavity flows with suppression using jet blowing显示文摘 | ALISON M L CHOKANI N | 1997 | Journal of Aircraft1997，34，4： | 1 |
| 18 | The increasing prevalence of autismspectrum disorders 显示文摘 | Johnny L Alison M | 2011 | Res Autism Spect Dis2011，，5： | 1 |
| 19 | Use of at- line and in-situ near-infrared spectroscopy to monitor biomass in anindustrial fed-batch Escherichia coli process显示文摘 | Alison A S Rumpai G Harvey L M | 2002 | Biotechnologyand Bioengineering2002，80，4： | 1 |
| 20 | Distinction between differentiation, cell cycle, and apoptosis signals in PC12 cells by the nerve growth factor mutant A9/13,which is selective for the p75 neurotrophin receptor 显示文摘 | Alison L H Debbie M J Shivanand P L | 2001 | J Neurosei Res2001，63，1： | 1 |
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