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| 1 | An integrated approach for increasing breeding efficiency in apple and peach in Europe显示文摘Despite the availability of whole genome sequences of apple and peach,there has been a considerable gap between genomics and breeding.To bridge the gap,the European Union funded the FruitBreedomics project(March 2011 to August 2015)involving 28 research institutes and private companies.Three complementary approaches were pursued:(i)tool and software development,(ii)deciphering genetic control of main horticultural traits taking into account allelic diversity and(iii)developing plant materials,tools and methodologies for breeders.Decisive breakthroughs were made including the making available of ready-to-go DNA diagnostic tests for Marker Assisted Breeding,development of new,dense SNP arrays in apple and peach,new phenotypic methods for some complex traits,software for gene/QTL discovery on breeding germplasm via Pedigree Based Analysis(PBA).This resulted in the discovery of highly predictive molecular markers for traits of horticultural interest via PBA and via Genome Wide Association Studies(GWAS)on several European genebank collections.FruitBreedomics also developed pre-breeding plant materials in which multiple sources of resistance were pyramided and software that can support breeders in their selection activities.Through FruitBreedomics,significant progresses were made in the field of apple and peach breeding,genetics,genomics and bioinformatics of which advantage will be made by breeders,germplasm curators and scientists.A major part of the data collected during the project has been stored in the FruitBreedomics database and has been made available to the public.This review covers the scientific discoveries made in this major endeavour,and perspective in the apple and peach breeding and genomics in Europe and beyond. | Francois Laurens Maria JoséAranzana Pere Arus Daniele Bassi Marco Bink Joan Bonany Andrea Caprera Luca Corelli-Grappadelli Evelyne Costes Charles-Eric Durel Jehan-Baptiste Mauroux Hélène Muranty Nelson Nazzicari Thierry Pascal Andrea Patocchi Andreas Peil Bénédicte Quilot-Turion Laura Rossini Alessandra Stella Michela Troggio Riccardo Velasco Eric van de Weg | 2018 | Horticulture Research2018，5，1： | 10 |
| 2 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 3 | 6,7-Dihydroxy-1,2,3,4-tetrahydroisoquinoline formation by iron mediated dopamine oxidation:a nivel route to endogenous neurotoxins under oxidative stress condition显示文摘 | Alessandra N Alessandro P Giuseppe P | 1999 | Tetrahedron Letters1999，40，14： | 1 |
| 4 | Poly(hydroxylbutyrate-co-hydroxyvalerate) bilayer skin tissue engineering constructs with improved epidermal rearrange-ment显示文摘 | Alessandra Z Mariana C T Silviene N | 2014 | Macromolecular Bioscience2014，14，7： | 1 |
| 5 | Advanced oxidation of the pharmaceutical drug diclofenac with UV/H2O2 and ozone显示文摘 | V Davide M Raffaele N R Alessandra | 2004 | Water Res2004，38，2： | 1 |
| 6 | Survey on LBP based texture descriptors for image classification显示文摘 | Loris N Alessandra L Sheryl B | 2012 | Expert Systems with Applications2012，39，： | 1 |
| 7 | A novel chemometric strategy for the estimation of extra virgin olive oil adulteration with edible oils显示文摘 | Rubén M. Maggio Lorenzo Cerretani Emma Chiavaro Teodoro S. Kaufman Alessandra Bendini | 2009 | Food Control2009，，6： | 1 |
| 8 | An ensemble of support vector machines for predicting the membrane protein type directly from the amino acid sequence显示文摘 | Loris N Alessandra L | 2008 | Amino Acids2008，35，3： | 1 |
| 9 | Outcome of hyperleukocytic adult acute myeloid leukaemia:A single-center retrospective study and review of literature显示文摘 | GUIDO N MICHELE N ALESSANDRA T | 2008 | Leukemia Res2008，32，： | 1 |
| 10 | Radiofrequency ablation for the treatment of radiation proctitis显示文摘 | Xavier Dray Gorgio Battaglia Dov Wengrower Pedro Gonzalez Alessandra Carlino Marine Camus Tomer Adar Francisco Pérez-Roldán Philippe Marteau Alessandro Repici | 2014 | Endoscopy2014，，： | 1 |
| 11 | Branehed-ehain amino acids, mitochondrial biogenesis, and healthspan: an ewslu- tionaly perspective显示文摘 | ALESSANDRA V GIUSEPPE DA ENZO N | 2011 | Aging2011，3，5： | 1 |
| 12 | Local binary patterns variants as texture descriptors for medical image analysis显示文摘 | LORIS N ALESSANDRA L SHERYL B | 2010 | Artificial Intelligence in Medicine2010，49，： | 1 |
| 13 | Survey on LBP based texture descriptors for image classification 显示文摘 | Lofts N Sheryl B Alessandra L | 2012 | Expert Systems with Applications2012，39，3： | 1 |
| 14 | Heterogeneous bag-of-features for object/scene recognition显示文摘 | Loris N Alessandra L | 2013 | Applied Soft Computing2013，13，4： | 1 |
| 15 | Association between clusters of diseases and polypharmacy in hospitalized elderly pa- tientsResults from the REPOSI study显示文摘 | Alessandro N Alessandra M Mauro T | 2011 | Eur J Inter Med2011，22，6： | 1 |
| 16 | Survey on LBP based texture descriptors for image classification 显示文摘 | Loris N Alessandra L Sheryl B | 2012 | Expert Systems with Applications2012，39，： | 1 |
| 17 | α-synuclein and Parkinson's disease显示文摘 | Alessandra R Patrizia D Alessandra N | 2004 | FASEB J2004，18，6： | 1 |
| 18 | Α-Synuclein and Parkinson's disease显示文摘 | Alessandra R Patrizia D Alessandro N | 2004 | FASEB2004，18，： | 1 |
| 19 | Survey on LBP based texture descriptors for image classification 显示文摘 | Loris N Alessandra L Sheryl B | | Expert System with Applications0，39，3： | 1 |
| 20 | Outcome Compari- son Among Laparoscopic Duhamel, Laparotomic Duhamel, and Transanal Endorectal Pull-Through: A Single-Center, 18-Year Experience 显示文摘 | Stefano G Pietro B Alessandra N | 2011 | J Laparoendosc Adv Surg Tech2011，21，4： | 1 |
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