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| 1 | Foveal avascular zone area and parafoveal vessel density measurements in different stages of diabetic retinopathy by optical coherence tomography angiography显示文摘AIM:To investigate foveal avascular zone(FAZ) and parafoveal vessel densities(PRVD) by means of optical coherence tomography angiography(OCTA) in diabetic patients with or without diabetic retinopathy(DR) and to assess the reproducibility of FAZ and PRVD measurements.METHODS:Sixty diabetic patients(60 eyes) with different stage of DR(graded according to the International Clinical Severity Scale for DR) and 20 healthy subjects underwent FAZ area and PRVD measurements using OCTA by two experienced examiners.FAZ area in all patients was also assessed using fluorescein angiography(FA).RESULTS:In subject with proliferative DR and with moderate-severe non proliferative DR,FAZ area was significantly increased compared to healthy controls(P=0.025 and P=0.050 respectively measured with OCTA and P=0.025 and P=0.048 respectively measured with FA).OCTA showed significantly less inter-observer variability compared to FA.Concordance correlation coefficient(CCC) for FAZ area measurements was 0.829(95%CI:0.736-0.891) P<0.001 with FA and 1.000(95%CI:0.999-1.000) P<0.001 with OCTA.CCC was 0.834(95%CI:0.746-0.893) P<0.001 and 0.890(95%CI:0.828-0.930) P<0.001 for parafoveal superficial and deep vessel density measurements,respectively.CONCLUSION:OCTA shows progressive increase of FAZ area and reduction of PRVD in both superficial and deep plexus at increasing DR severity.FAZ area and PRVD measurements using OCTA are highly reproducible. | Rodolfo Mastropasqua Lisa Toto Alessandra Mastropasqua Raffaella Aloia Chiara De Nicola Peter A Mattei Guido Di Marzio Marta Di Nicola Luca Di Antonio | 2017 | International Journal of Ophthalmology(English edition)2017，10，10： | 26 |
| 2 | Pattern response of dendritic cells in the tumor microenvironment and breast cancer显示文摘Breast cancer(BC) is the most common malignant neoplasm and the cause of death by cancer among women worldwide. Its development, including malignancy grade and patient prognosis, is influenced by various mutations that occur in the tumor cell and by the immune system's status, which has a direct influence on the tumor microenvironment and, consequently, on interactions with non-tumor cells involved in the immunological response. Among the immune response cells, dendritic cells(DCs) play a key role in the induction and maintenance of anti-tumor responses owing to their unique abilities for antigen cross-presentation and promotion of the activation of specific lymphocytes that target neoplasic cells. However, the tumor microenvironment can polarize DCs, transforming them into immunosuppressive regulatory DCs, a tolerogenicphenotype which limits the activity of effector T cells and supports tumor growth and progression. Various factors and signaling pathways have been implicated in the immunosuppressive functioning of DCs in cancer, and researchers are working on resolving processes that can circumvent tumor escape and developing viable therapeutic interventions to prevent or reverse the expression of immunosuppressive DCs in the tumor microenvironment. A better understanding of the pattern of DC response in patients with BC is fundamental to the development of specific therapeutic approaches to enable DCs to function properly. Various studies examining DCs immunotherapy have demonstrated its great potential for inducing immune responses to specific antigens and thereby reversing immunosuppression and related to clinical response in patients with BC. DCbased immunotherapy research has led to immense scientific advances, both in our understanding of the antitumor immune response and for the treatment of these patients. | Alessandra da Cunha Marcia A Michelin Eddie FC Murta | 2014 | World Journal of Clinical Oncology2014，5，3： | 5 |
| 3 | Antiherpetic activity of a sulfated polysaccharide from Agaricus brasiliensis mycelia显示文摘 | Francielle Tramontini Gomes de Sousa Cardozo Carla Maísa Camelini Alessandra Mascarello Márcio José Rossi Ricardo José Nunes Célia Regina Monte Barardi Margarida Matos de Mendon?a Cláudia Maria Oliveira Sim?es | 2011 | Antiviral Research2011，，1： | 2 |
| 4 | Influence of donor age on the differentiation and division capacity of human adipose-derived stem cells显示文摘BACKGROUND Human adipose-derived stromal/stem cells(hASCs)are one of the most useful types of mesenchymal stromal/stem cells,which are adult multipotent cells with great therapeutic potential for the treatment of several diseases.However,for successful clinical application,it is critical that high-quality cells can be obtained.Diverse factors seem to be able to influence cell quality and performance,especially factors related to donors’intrinsic characteristics,such as age.Nevertheless,there is no consensus regarding this characteristic,and there is conflicting information in the literature.AIM To investigate the growth kinetics and differentiation potential of adipose-derived stem cells isolated from the lipoaspirates of elderly and young donors.METHODS hASCs were harvested from liposuctioned adipose tissue obtained from female donors(aged 20-70 years).Cells were distributed into two groups according to age range:old hASCs(oASCs,≥55 years,n=9)and young hASCs(yASCs,≤35 years,n=9).For each group,immunophenotypic characterization was performed by flow cytometry.Population doubling time was assessed over seven days.For adipogenic potential evaluation,lipid deposits were assessed after 7 d,14 d and 21 d of adipogenic induction.Osteogenic potential was verified by analyzing cell mineralization after 14 d,21 d and 28 d of osteogenic induction.mRNA expression of PPARγ2,CEBPA and Runx2 were detected by quantitative reverse transcription polymerase chain reaction.RESULTS hASCs were successfully obtained,cultured,and grouped according to their age:yASCs(26.33±4.66 years old)and oASCs(64.78±4.58 years old).After maintenance of the cells in culture,there were no differences in morphology between cells from the young and old donors.Additionally,both groups showed classical immunophenotypic characteristics of mesenchymal stem/stromal cells.The average doubling time indicated that yASCs(4.09±0.94 d)did not significantly differ from oASCs(4.19±1.29 d).Concerning differentiation potential,after adipogenic and osteogenic induction,yASCs and oASCs were able to differentiate to greater levels than the noninduced control cells.However,no differences were found in the differentiation efficiency of yASCs and oASCs in adipogenesis or osteogenesis.Additionally,the mRNA expression of PPARγ2,CEBPA and Runx2 were similar in yASCs and oASCs.CONCLUSION Our findings suggest that age does not seem to significantly affect the cell division or adipogenic or osteogenic differentiation ability of adipose-derived stem cells isolated from lipoaspirates. | Cintia DS Horinouchi María Julia Barisón Anny W Robert Crisciele Kuligovski Alessandra M Aguiar BrunoDallagiovanna | 2020 | World Journal of Stem Cells2020，12，12： | 2 |
| 5 | Molecular imaging of movement disorders显示文摘Positron emission tomography measures the activity of radioactively labeled compounds which distribute and accumulate in central nervous system regions in proportion to their metabolic rate or blood flow. Specific circuits such as the dopaminergic nigrostriatal projection can be studied with ligands that bind to the pre-synaptic dopamine transporter or post-synaptic dopamine receptors(D1 and D2). Single photon emission computerized tomography(SPECT) measures the activity of similar tracers labeled with heavy radioactive species such as technetium and iodine. In essential tremor, there is cerebellar hypermetabolism and abnormal GABAergic function in premotor cortices, dentate nuclei and ventral thalami, without significant abnormalities in dopaminergic transmission. In Huntington's disease, there is hypometabolism in the striatum, frontal and temporal cortices. Disease progression is accompanied by reduction in striatal D1 and D2 binding that correlates with trinucleotide repeat length, disease duration and severity. In dystonia, there is hypermetabolism in the basal ganglia, supplementary motor areas and cerebellum at rest. Thalamic and cerebellar hypermetabolism is seen during dystonic movements, which can be modulated by globus pallidus deep brain stimulation(DBS). Additionally, GABA-A receptor activity is reduced in motor, premotor and somatosensory cortices. In Tourette's syndrome, there is hypermetabolism in premotor and sensorimotor cortices, as well as hypometabolism in the striatum, thalamus and limbic regions at rest. During tics, multiple areas related to cognitive, sensory and motor functions become hypermetabolic. Also, there is abnormal serotoninergic transmission in prefrontal cortices and bilateral thalami, as well as hyperactivity in the striatal dopaminergic system which can be modulated with thalamic DBS. In Parkinson's disease(PD), there is asymmetric progressive decline in striatal dopaminergic tracer accumulation, which follows a caudal-to-rostral direction. Uptake declines prior to symptom presentation and progresses from contralateral to the most symptomatic side to bilateral, correlating with symptom severity. In progressive supranuclear palsy(PSP) and multiple system atrophy(MSA), striatal activity is symmetrically and diffusely decreased. The caudal-to-rostral pattern is lost in PSP, but could be present in MSA. In corticobasal degeneration(CBD), there is asymmetric, diffuse reduction of striatal activity, contralateral to the most symptomatic side. Additionally, there is hypometabolism in contralateral parietooccipital and frontal cortices in PD; bilateral putamen and cerebellum in MSA; caudate, thalamus, midbrain, mesial frontal and prefrontal cortices in PSP; and contralateral cortices in CBD. Finally, cardiac sympathetic SPECT signal is decreased in PD. The capacity of molecular imaging to provide in vivo time courses of gene expression, protein synthesis, receptor and transporter binding, could facilitate the development and evaluation of novel medical, surgical and genetic therapies in movement disorders. | Karlo J Lizarraga Alessandra Gorgulho Wei Chen Antonio A De Salles | 2016 | World Journal of Radiology2016，8，3： | 2 |
| 6 | Structured zeolite NaX coatings on ceramic cordierite monolith supports for PSA applications显示文摘 | Alessandra Mosca Jonas Hedlund Paul A Webley | 2010 | Microp Mesop Mater2010，130，： | 1 |
| 7 | Repartition effect of aromatic polyaniline coatings on the separation of bioactive peptides in capillary electrophoresis显示文摘 | ALESSANDRA B SERGEY A P ANTHONY P F T PIER G R | 2002 | Electrophoresis2002，23，2： | 1 |
| 8 | Partial ox- idation of light paraffins to synthesis gas in short con- tact-time reactors 显示文摘 | ALESSANDRA BERETI'A PIO FORZATI'I | 2004 | Chemical Engineering Journal2004，99，3： | 1 |
| 9 | Enantioselective 1,3-dipolar cycloadditions of unsaturated aldehydes promoted by apoly( ethylene glycol)-supported organic catalyst显示文摘 | Alessandra P M aurizio B M auro C e t a l | 2004 | E ur J Org Chem2004，344，： | 1 |
| 10 | Evidence for a biphasic apoptotic pathway induced by melatonin in MCF-7 breast cancer cells显示文摘 | Alessandra C Sara P Fabrizio D A | 2009 | J Pineal Res2009，46，3： | 1 |
| 11 | Expression of CXC chemokine receptors 1-C5 and their ligands in human glioma tissues:Role of CXCR4 and SDFI in glioma cell proliferation and migration显示文摘 | Adriana B A Federiea B Alessandra D B | 2006 | Neurochemistry International2006，49，5： | 1 |
| 12 | Probabilistic approach for determining Salmonella spp. and L. monocytogenes concentration in pork meat from presence/absence microbiological data显示文摘 | Antonio Valero Marta Hernandez Alessandra De Cesare Gerardo Manfreda Rosa Maria García-Gimeno Patricia González-García David Rodríguez Lázaro | 2014 | International Journal of Food Microbiology2014，，： | 1 |
| 13 | Determination of the behaviour factor of steel moment-resisting (MR) frames by a damage accumulation approach 显示文摘 | Carlo A C Alessandra Z | 2010 | Journal of Constructional Steel Research2010，66，5： | 1 |
| 14 | Evalua- tion of correct endogenous reactive oxygen species content for human sperm capacitation and involve- ment of the NADPH oxidase system显示文摘 | Gabriella D Cristina F Alessandra A | 2011 | Human Re- production2011，26，12： | 1 |
| 15 | Application of a spectroscopic method to estimate the olive oil oxidative status 显示文摘 | Sarfaraz A M Alessandra B Lorenzo C | 2010 | European Journal of Lipid Science and Technolo- gy2010，112，12： | 1 |
| 16 | Hypoxia-inducible factor induces local thyroid hormone inactivation during hypoxic-ischemic disease in rats显示文摘 | Simonides Warner S Mulcahey Michelle A Redout Everaldo M Muller Alice Zuidwijk Marian J Visser Theo J Wassen Frank W J S Crescenzi Alessandra da-Silva Wagner S Harney John Engel Felix B Obregon Maria-Jesús Larsen P Reed Bianco Antonio C Huan | 2008 | Journal of Clinical Investigation2008，，3： | 1 |
| 17 | Evaluation of Corophium orientale as bioindicator for Venice Lagoon: sensitivity assessment and toxicity-score proposal显示文摘 | Picone M Bergamin M Novelli Alessandra A | 2008 | Ecotoxicology and Environmental Safety2008，70，： | 1 |
| 18 | US-- elastography in the differential diagnosis of benign and ma- lignant thyroid nodules显示文摘 | CARMELA A ALESSANDRA G ALESSANDRO P | 2008 | Thyroid2008，18，3： | 1 |
| 19 | Endogenous and exogenous poly- amines in the organogenesis in Curcuma longa 显示文摘 | Alessandra F M Viu Marco A O Viu Armando R Tavares | 2009 | Sci Hortieulturae2009，121，4： | 1 |
| 20 | Hepcidin in obese children as a potential mediator of the asso- ciation between obesity and iron deficiency 显示文摘 | MIRAGLIA-DEL G E NICOLA S ALESSANDRA A | 2009 | J Clin Endo- crinol Metab2009，94，12： | 1 |
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