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| 1 | Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease显示文摘AIM:To investigate intestinal alkaline phosphatase(iAP) in the intestinal mucosa of children with inflammatory bowel disease(IBD).METHODS:Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls.In IBD patients,specimens were obtainedboth from inflamed and non-inflamed areas.The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis,respectively.Tissue localization of iAP and Toll-like receptor(TLR) 4 was investigated by immunofluorescent staining.RESULTS:The iAP protein level in the inflamed mucosa of children with Crohn's disease(CD) and ulcerative colitis(UC) was significantly decreased when compared with controls(both P < 0.05).Similarly,we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD(P < 0.05).In addition,the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD(P < 0.05).iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls.iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD.Expression of iAP mRNA in patients with noninflamed mucosa and in controls were similar.Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern.iAP was present in the inflamed and non-inflamed mucosa of patients with CD,UC,and in control biopsy specimens,irrespective of whether it was present in the terminal ileum or in the colon.However,the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.CONCLUSION:Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD.Based on our results,administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option. | Kriszta Molnár dám Vannay Beáta Szebeni Nóra Fanni Bánki Erna Sziksz ron Cseh Hajnalka Gyrffy Péter László Lakatos Mária Papp András Arató Gábor Veres | 2012 | World Journal of Gastroenterology2012，18，25： | 5 |
| 2 | Increased duodenal expression of mi R-146a and-155 in pediatric Crohn's disease显示文摘AIM: To evaluate the role of micro RNA(mi R)-146 a,-155 and-122 in the duodenal mucosa of pediatric patients with Crohn's disease(CD) and the effect of transforming growth factor-β(TGF-β) on these mi Rs in duodenal epithelial and fibroblast cells.METHODS: Formalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed(CD inflamed: n = 10) and intact(CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children(C: n = 10) were examined. Expression of mi R-146 a,-155 and-122 was determined by realtime polymerase-chain reaction(PCR). The expression of the above mi Rs was investigated in recombinant human TGF-β(1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells(CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.RESULTS: Expression of mi R-146 a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD(CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, p ≤ 0.01) and to the control group(CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, p ≤ 0.05). The expression of mi R-155 was significantly increased in the inflamed region of the duodenum compared to the control group(CD inflamed: 4.87 ± 1.02 vs Control: 1.00 ± 0.40, p ≤ 0.001). The expression of mi R-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of mi R-155 in small intestinal epithelial cells(TGF-β: 0.7 ± 0.083 vs Control: 1 ± 0.09, p ≤ 0.05) and also the expression of mi R-146a(TGF-β: 0.67 ± 0.04 vs Control: 1 ± 0.15, p ≤ 0.01) and mi R-155(TGF-β: 0.72 ± 0.09 vs Control: 1 ± 0.06, p ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of mi R-122.CONCLUSION: The elevated expression of mi R-146 a and-155 in the inflamed duodenal mucosa of CD patients suggests the role of these mi Rs in the pathomechanism of inflammatory bowel disease. Antiinflammatory TGF-β plays an important role in the regulation of the expression of these mi Rs. | Dániel Szucs Nóra Judit Béres Réka Rokonay Kriszta Boros Katalin Borka Zoltán Kiss András Arató Attila J Szabó ádám Vannay Erna Sziksz Csaba Bereczki Gábor Veres | 2016 | World Journal of Gastroenterology2016，22，26： | 2 |
| 3 | Live three-dimensional transthoracic echocardiography in the assessment of the papillary muscle abnormality with mitral regurgitation 显示文摘 | Arat N Golbasi Z Ilkay E | 2007 | Anadolu Kardiyol Derg2007，7，4： | 1 |
| 4 | Elastic properties of aorta are impaired in patients with slow coronary flow phenomen on显示文摘 | Arat N Altay H Sabah I | 2008 | Indian Heart J2008，60，2： | 1 |
| 5 | Persistent left superior vena cavawith absent right superior vena cava and coronary sinus dilationmimicking a paracardiac mass显示文摘 | Arat N Sokmen Y Golbasi Z | 2007 | Tex Heart Inst J2007，34，4： | 1 |
| 6 | A phase I dose escalation study of high-dose thiotepa, melphalan and carboplatin (TMCb) followed by autologous peripheral blood stem cell transplantation (PBSCT) in patients with solid tumors and hematologic malignancies 显示文摘 | Ilhan O Mandel NM Arat M Gunel N Celebi H | 2000 | Bone Marrow Transplant2000，25，7： | 1 |
| 7 | Iatrogenic silent right atrial dissection and rupture显示文摘 | ARAT N ALTAY H ULUPINAR H | 2006 | Can J Cardiol2006，22，14： | 1 |
| 8 | Right ven- trieular and septal function in patients with pulmonary hyper- tension显示文摘 | ULUS A T POYRAZ N Y ARAT N A | 2013 | Heart Lung Circ2013，22，12： | 1 |
| 9 | Noninvasive assessment ofmyocardial bridging in the left coronary artery by transthoracicDoppler echocardiography显示文摘 | Arat N Altay H Yildirim N | 2007 | Eur J Echocardiogr2007，8，4： | 1 |
| 10 | Left and right atrial myocardial deformation properties in patients with an atrial septal defect显示文摘 | Arat N Sokmen Y Altay H | 2008 | J Echocardiogr2008，25，4： | 1 |
| 11 | Comparison of traditional risk factors, natural history and angiographic findings between coro- nary heart disease patients with age < 40 and > 40 years old 显示文摘 | Yildifim N Arat N Dogan MS | 2007 | Anadolu Kardiyol Derg2007，7，2： | 1 |
| 12 | Triad of malnutrition, inflammation, and atheroselerosis in hemodialysis patients 显示文摘 | SEZER S OZDEMIR F N ARAT Z | 2002 | Nephron2002，91，3： | 1 |
| 13 | The effect of baseline pulmonary artery pressure on right ventricular functions after mitral balloon valvuloplasty for rheumatic mitral stenosis: a tissue Doppler imaging study显示文摘 | Arat N Altay H Korkmaz S | 2008 | Turk Km''diyol Dern Ars2008，36，4： | 1 |
| 14 | Algorithmic aspects of hardwaresoftware partitioning显示文摘 | Arató P Mann Z Orbán A | 2005 | ACM Transactions on Design Automation of Electronic Systems2005，10，1： | 1 |
| 15 | What happens after conversion of treatment to continuous ambulatory peritoneal dialysis from hemodialysis?显示文摘 | Sezer S Ozdemir N Arat Z | 2000 | Adv Perit Dial2000，16，： | 1 |
| 16 | Microprogrammed logic network design original research article显示文摘 | Zoltán László Péter Arató | | 0，，02： | 1 |
| 17 | Elastic properties of aorta are impaired in patients with slow coronary flow phenomenon 显示文摘 | Arat N Altay H Sabah I | 2008 | Indian Heart J2008，60，2： | 1 |
| 18 | Detection and Quantification of genotoxicity in wastewater-treated Tetrahymena thermophila显示文摘 | Lah B Malovrh S N arat M | 2004 | Environmental Toxicology2004，19，6： | 1 |
| 19 | A case of simultaneous anterior, inferior, and right ventricular ST-segment elevation myocardial infarction due to occlusion of the wrapped left anterior descending coronary artery 显示文摘 | Akpinar I Arat N Ozbulblll NI ct al | 2008 | Turk Kardiyol Dem Ars2008，36，5： | 1 |
| 20 | The isolalion f a rickettsia - like organism causing disease md mortaliIy in Chilean stdmonids mid its confimmtion by Kthg istulates显示文摘 | CVITANIi]H j D t ARATE N O SMrFII C E | 1991 | J Fish Dis1991，14，： | 1 |
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