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| 1 | Expression of sialyl Lewis^a relates to poor prognosis in cholangiocarcinoma显示文摘AIM: High levels of serum sialyl Lewisa (sLea) are frequently found in cholangiocarinnoma (CCA) patients and have been suggested to be a serum marker for CCA. However, the significance of this antigen in CCA is unknown. In this study,the clinical significance of sLea expression in CCA tissues and the possible role of sLea in vascular invasion in vitro were elucidated.METHODS: Expression of sLea in tumor tissues of 77patients with mass-forming CCA and 33 with periductal infiltrating CCA was determined using immunohistochemistry.The in vitro assays on adhesion and transmigration of CCA cells to human umbilical vein endothelial cells were compared between CCA cell lines with and without sLea expression.RESULTS: sLea was aberrantly expressed in 60% of CCA tumor tissues. A significant relationship was found between the frequency of sLea expression and the mass-forming type CCA (P = 0.041), well differentiated histological grading (P = 0.029), and vascular invasion (P = 0.030). Patients with positive sLea expression had a significantly poorer prognosis (21.28 wk, 95% CI = 16.75-25.81 wk) than those negative for sLea (37.30 wk, 95% CI = 27.03-47.57 wk)(P<0.001). Multivariate analysis with adjustment for all covariates showed that patients positive for sLea possessed a 2.3-fold higher risk of death than patients negative for sLea (P<0.001). The role of sLea in vascular invasion was demonstrated using in vitro adhesion and transmigration assays. KKU-M213, a human CCA cell-line with a high expression of sLea, adhered and transmigrated to IL-1β-activated endothelial cells of the human umbilical vein more than KKU-100, the line without sLea expression (P<0.001).These processes were significantly diminished when the antibodies specific to either sLea or E-selectin were added to the assays (P<0.001).CONCLUSION: This study demonstrates the clinical significance of sLea expression in vascular invasion, and an unfavorable outcome in CCA. The role of sLea in vascular invasion which may lead to poor prognosis is supported by the in vitro adhesion and transmigration studies. | Apa Juntavee Banchob Sripa Ake Pugkhem Narong Khuntikeo Sopit Wongkham | 2005 | World Journal of Gastroenterology2005，11，2： | 2 |
| 2 | 甲氨蝶呤治疗前后银屑病皮损中ICAM-3、Ki-67、PCNA及CD31表达的变化显示文摘Although the effectiveness of methotrexate (MTX) in the treatment of psoriasis is very well established, the mechanism of action is poorly understood. It was suggested that the therapeutic effect of MTX in psoriasis might be mediated by inhibition of adhesion molecule expression. The aim of our study was to investigate the different effects of MTX treatment on cell proliferation, inflammatory infiltrate, adhesion molecules, and angiogenesis in psoriasis, and to clarify the mechanism by which MTX exerts its therapeutic effects. Clinical response, the morpho- phenot- ypic changes, epidermal thickness, and mitosis count were analyzed and the expression of CD31 and ICAM- 3, proliferative markers such as Ki- 67, PCNA, were evaluated by immunohistochemical techniques in lesional psoriatic epidermis, before and after the treatment with MTX in ten patients. In posttreatment biopsies a decrease in the degree of epidermal hyperplasia and a significant reduction in the severity of the inflammatory infiltrate (P< 0.05) were observed. In addition,CD31 and ICAM- 3 expression was significantly decreased ondermal cellular infiltrate, (respectively; P< 0.05,P< 0.01). Ki67 and PCNA expression were suppressed concurrently in about 90% of cases (P< 0.01). We suggest that MTX may have an inhibitory effect on an initial integral component of the pathways that lead to psoriasis. Immunopharmacologic intervention in adhesion event has the potential to improve psoriasis. Inhibition of revascularization may be another mechanism of actionof MTX. | Yazici A.C. Tursen U. Apa D.D. 潘敏 | 2006 | 世界核心医学期刊文摘(皮肤病学分册)2006，0，4： | 2 |
| 3 | Evidence- based practice in psychology 显示文摘 | APA Presidenti~ Task Force on Evidence-Based Practice | 2006 | Am Psychol2006，61，4： | 1 |
| 4 | Avian encephalomyelitis virus is picornavirus and is most closely related to hepatitis A virus显示文摘 | Marvil P Knowles NJ Mockett APA | 1999 | J G en Virol1999，80，： | 1 |
| 5 | Evaluation of chronic cough in children显示文摘 | Asilsoy S Bayram E Agin H Apa H Can D Gulle S | 2008 | Chest2008，134，6： | 1 |
| 6 | The effect of N-acetylcysteine(NAC) on liver and renal tissue inducible nitric oxide synthase(iNOS) and tissue lipid peroxidation in obstructive jaundice stimulated by lipopolysaccharide(LPS)显示文摘 | CAGLIKULEKCI M PATA C APA D D | 2004 | Pharmacol Res2004，49，3： | 1 |
| 7 | What DRN members do in disasterresponsel American Psychological Association's Disaster Response显示文摘 | APA DRN Advisory Committee | 2002 | Network News2002，8，1： | 1 |
| 8 | he changes in expression of ICAM-3,Ki-67,PCNA, and CD31 in psoriatic lesions before and after methotrexate treatment 显示文摘 | Yazici AC Tursen U Apa DD | 2005 | Arch Dermatol Res2005，297，6： | 1 |
| 9 | The changes in expression of ICAM-3, Ki-67, PCNA, and CD31 in psoriatic lesions before and after methotrexate treatment 显示文摘 | Yazici AC Tursen U Apa DD | 2005 | Arch Dermatol Res2005，297，6： | 1 |
| 10 | Usefulness of 3D transperineal ultrasound in severe stenosis of the anal canal: preliminary experience in four cases显示文摘 | M. Ko?odziejczak G. A. Santoro R. Z. S?apa T. Szopiński I. Sudo?-Szopińska | 2014 | Techniques in Coloproctology2014，，： | 1 |
| 11 | The changes in expression of ICAM-3,Ki-67,PCNA,and CD31 in psoriatic lesions before and after methotrexate treatment显示文摘 | Yazici AC Tursen U Apa DD | 2005 | Arch Dermatol Res2005，297，： | 1 |
| 12 | LC-MS Analysis of Antioxidant Plant Phenoloids显示文摘 | I Papp P Apa'ti V Andrasek A Bla'zovics | 2004 | Chromatographia Supplement2004，60，： | 1 |
| 13 | Pituitary adenylate cyclase-activating polypeptide modulates plasminogen activator expression in rat granulosa cell显示文摘 | Apa R Lanzone A Miceli F | 2002 | Biol Reprod2002，66，3： | 1 |
| 14 | Evidence -based practice in psychology显示文摘 | APA Presidential Task Force on Evidence-based Practice | 2006 | American Psychologist2006，61，4： | 1 |
| 15 | IAP/ APA evidence based guidelines for the management of acute pancreatitis 显示文摘 | Working Group 1AP/APA Acute Pancreatitis Guidelines | 2013 | Pancreatology2013，13，42： | 1 |
| 16 | The changes in expression of ICAM-3,Ki-67, PCNA, and CD31 in psoriatic lesions before and after methotrexate treatment 显示文摘 | Yazici A C Tursen U Apa D D | 2005 | Arch Dermatol Res2005，297，6： | 1 |
| 17 | Technical recommendations for psychological tests and diagnostic techniques 显示文摘 | APA | 1954 | Psychological Bulletin Supplement1954，5，2： | 1 |
| 18 | American Psychologist显示文摘 | APA Task Force on Evidence-Based Practice: Evidence-Based Practice in Psychology | 2006 | (5-6)2006，，56： | 1 |
| 19 | IAP/APA evidence-based guidelines for the management of acute pancreatitis显示文摘 | Working Group IAP/APA Acute Pancreatitis Guidelines | 2013 | Pancreatology2013，13，42： | 1 |
| 20 | Practice guideline for the treatment of patients with major depressive disorder(revision)显示文摘 | APA | 2000 | Am J Psychiatry2000，157，4： | 1 |
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