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| 1 | A modified β‐amyloid hypothesis: intraneuronal accumulation of the β‐amyloid peptide – the first step of a fatal cascade显示文摘 | OliverWirths GerdMulthaup Thomas A.Bayer | 2004 | Journal of Neurochemistry2004，，3： | 1 |
| 2 | THE DISC DAMAGE LIKELIHOOD SCORE A USEABLE TOOL IN THE DIAGNOSIS AND MANAGEMENT OF GLAUCOMA显示文摘Purpose: To discuss the value of a new method of grading quantitatively the amount of disc damage in patients with glaucoma Methods: The Disc Damage Likelihood Score (DDLS) employs 9 stages of damage based on the width of the neuroretinal rim or the circumferential extent of absence of rim, adjusted for the size of the optic disc. We report the results of studies regarding re- | G.L.Spaeth J.D.Henderer A.Bayer | 2003 | 国际眼科杂志2003，3，1： | 0 |
| 3 | Cellulosomal hemicellulases:Indispensable players for ensuring effective lignocellulose bioconversion显示文摘The bioconversion of lignocellulose has attracted global attention,due to the significant potential of agricultural and forestry wastes as renewable zero-carbon resources and the urgent need for substituting fossil carbon.The cellulosome system is a multi-enzyme complex produced by anaerobic bacteria,which comprises cellulases,hemicellulases,and associated enzymatic and non-enzymatic components that promote biomass conversion.To enhance their efficiency in degrading recalcitrant lignocellulosic matrices,cellulosomes have been employed to construct biocatalysts for lignocellulose bioconversion,such as consolidated bioprocessing and consolidated bio-saccharification.Hemicelluloses,the second most abundant polysaccharides in plant cell walls,hold valuable application potential but can also induce inhibitory effects on cellulose hydrolysis,thus highlighting the indispensable roles of hemicellulases within the cellulosome complex.This review evaluated current research on cellulosomal hemicellulases,comparing their types,abundance,and regulation,primarily focusing on eight known cellulosome-producing species of different origins.We also reviewed their growth conditions,their hemicellulose-degrading capabilities,and the inhibitory effects of hemicellulose on cellulosome-based lignocellulose saccharification.Finally,we proposed strategies for targeted enhancement of hemicellulase in cellulosomes to improve lignocellulose bioconversion in future studies. | Min Xiao Ya-Jun Liu Edward A.Bayer Akihiko Kosugi Qiu Cui Yingang Feng | 2024 | Green Carbon2024，2，1： | 0 |
| 4 | Affinity-induced covalent protein-protein ligation via the SpyCatcherSpyTag interaction显示文摘Production of economically viable bioethanol is potentially an environmentally and financially worthwhile endeavor.One major source for fermentable sugars is lignocellulose.However,lignocellulosic biomass is difficult to degrade,owing to its inherent structural recalcitrance.Cellulosomes are complexes of cellulases and associated polysaccharide-degrading enzymes bound to a protein scaffold that can efficiently degrade lignocellulose.Integration of the enzyme subunits into the complex depends on intermodular cohesin-dockerin interactions,which are robust but nonetheless non-covalent.The modular architecture of these complexes can be used to assemble artificial designer cellulosomes for advanced nanotechnological applications.Pretreatments that promote lignocellulose degradation involve high temperatures and acidic or alkaline conditions that could dismember designer cellulosomes,thus requiring separation of reaction steps,thereby increasing overall process cost.To overcome these challenges,we developed a means of covalently locking cohesin-dockerin interactions by integrating the chemistry of SpyCatcher-SpyTag approach to target and secure the interaction.The resultant cohesin-conjugated dockerin complex was resistant to high temperatures,SDS,and urea while high affinity and specificity of the interacting modular components were maintained.Using this approach,a covalently locked,bivalent designer cellulosome complex was produced and demonstrated to be enzymatically active on cellulosic substrates.The combination of affinity systems with SpyCatcher-SpyTag chemistry may prove of general use for improving other types of protein ligation systems and creating unconventional,biologically active,covalently locked,affinity-based molecular architectures. | Jacob O.Fierer Omar E.Tovar-Herrera Jonathan Y.Weinstein Amaranta Kahn Sarah Moraïs Itzhak Mizrahi Edward A.Bayer | 2023 | Green Carbon2023，1，1： | 0 |
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