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Downregulation of retinoic acid receptor-β_z expression is linked to aberrant methylation in esophageal squamous cell carcinoma cell lines

查看全文 作　　者：Zhong-[1]MinLiu;[1]FangDing;Ming-[2]ZhouGuo;Li-[1]YongZhang;[1]MinWu;Zhi-[1]HuaLiu 高影响力作者 机构地区：[1]NationalLaboratoryofMolecularOncology,CancerInstitute,PekingUnionMedicalCollegeandChineseAcademyofMedicalSciences,Beijing,China;[2]DivisionofTumorBiology,DepartmentofOncology,TheSidneyKimmelComprehensiveCancerCenteratJohnsHopkins,Baltimore,MD,USA高影响力机构 出　　处：《World Journal of Gastroenterology》索引2004年第10卷第6期,共5页高影响力期刊 基　　金：Supported by China Key Program on Basic Research,G1998051021;the Chinese Hi-tech R&D program,2001AA231041;National Science Foundation of China,30170519 摘　　要：AIM:To study the role of hypermethylation in the loss of retinoic acid receptor β2(RARβ2) in esophageal squamous cell carcinoma (ESCC).METHODS:The role of hypermethylation in RARβ2 gene silencing in 6 ESCC cell lines was determined by methylation-specific PCR (MSP),and its methylation status was compared with RARβ2 mRNA expression by RT-PCR.The MSP results were confirmed by bisulfite sequencing of RARβ2promoter regions.RESULTS:Methylation was detected in 4 of the 6 cell lines,and the expression of RARβ2 was markedly downregulated in 3 of the 4 methylated cell lines. The expression of RARβ2 was restored in one RARβ2-downregulated cell line with the partial demethylation of promoter region of RARβ2 after 5-aza-2′-deoxycytidine (5-aza-dc) treatment.CONCLUSION:The methylation of the 5′ region may play an important role in the downregulation of RARβ2 in some ESCC cell lines, suggesting that multiple mechanisms contribute to the loss of RARβ2expression in ESCC cell lines.This study may have clinical applications for treatment and prevention of ESCC. 关 键 词：维甲酸受体β2 食管鳞状细胞癌 肿瘤细胞 甲基化 肿瘤生物学 细胞培养
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