维普中文期刊产品整合服务

Targeted expression of human FSH receptor Asp567Gly mutant mRNA in testis of transgenic mice: role of humanFSH receptor promoter

查看全文 作　　者：Verena [1]Nordhoff;Jrg [1]Gromoll;Luca [1]Foppiani;C.Marc [1]Luetjens;Stefan [1]Schlatt;Elena [1]Kostova;Ilpo [1]Huhtaniemi;Eberhard [1]Nieschlag;Manuela [1]Simoni 高影响力作者 机构地区：[1]Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Department of Physiology University of Turku, Kiinamyllynkatu 10, FIN-20520 Turku, Finland,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany,Institute of Reproductive Medicine University of Miinster, D-48149 Munster, Germany高影响力机构 出　　处：《Asian Journal of Andrology》索引2003年第5卷第4期,共9页高影响力期刊 摘　　要：Aim:To specifically express the Asp567Gly human follicle-stimulating hormone receptor (FSHR) under the control of its promoter to evaluate the phenotypic consequences in the presence of normal pituitary function.Methods:We produced transgenic mice overexpressing the Asp567Gly human FSHR under the control of a 1.5kb 5’-flanking region fragment of its promoter.Results: Mice were phenotypically normal and fertile.In males,mRNA could be detected in the testis and the brain, indicating that the 1.5kb promoter fragment drives expression not only in the gonads. The testis weight/body weight ratio and the testosterone levels in transgenic and non-transgenic littermates were similar. By in situ hybridisation we found that the transgenic FSHR was highly expressed in Sertoli cells,spermatocytes and round spermatids. However, a radioligand receptor assay failed to show a significant difference in total FSHR binding sites in testis homogenates of transgenic and wild type animals, suggesting that the transgenic FSHR is probably not translated into functional receptor protein. Conclusion: A 1.5kb 5'-region of the human FSHR drives mRNA expression of the transgene in the testis but leads to ectopic expression in germ cells and in the brain. No phenotypic consequences could be documented due to the lack of protein expression. 关 键 词：滤泡刺激激素受体 Asp567多聚糖 精子生成 垂体 动物实验 PCR
相关文献

参考文献(10)

引证文献(1)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费