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Effect of the RNA5 component on pathogenicity of beet necrotic yellow vein virus

查看全文 作  者:LIU Tao, HAN Chenggui, LI Dawei, CAI Zhunan, YU Jialin & LIU Yi State Key Laboratory for Agrobiotechnology and College of Biological Science, China Agricultural University, Beijing 100094, China Correspondence should be addressed to Yu Jialin (e-mail: bnyvv@public. bta.net.cn) 高影响力作者 出  处:《Chinese Science Bulletin》索引2003年第48卷第8期,共4页高影响力期刊 基  金:This work was supported by the State'863'High-Tech Program(Grant No.200]AA24]193). 摘  要:The transcripts of beet necrotic yellow virus RNA5 were prepared from the cloned cDNA in a bacterio-phage T7 in vitro run-off transcription system. The RNA5 transcripts and RNAs extracted from two BNYVV mutants, Hu0 containing RNA1, 2 and Hu3 containing RNA1, 2, 3 respectively, were co-inoculated to Tetragonia expansa and sugar beet seedlings. The results indicated that the virus infections and accumulation in the hosts could be improved by presence of RNA5. Cooperating with RNA3, the RNA5 seems to cause more severe symptom and yield losses in sugar beet. These results suggest that, in addition of RNA3, BNYVV RNA5 is another important factor associated with the virus pathogenicity. 关 键 词:RNA 致病性 甜菜 BNYVV 核糖核酸
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