大肠杆菌VT噬菌体受体(vpr)基因突变株的鉴定

查看全文 作　　者：[1]严亚贤;[1]孙建和;[2]陆承平;Heather E [3]Allison

机构地区：[1]上海交通大学农业与生物学院,上海201101;[2]南京农业大学农业部动物疫病诊断与免疫重点开放实验室,南京210095;[3]英国利物浦大学生物科学院环境与分子微生物系

出　　处：《微生物学报》索引2002年第42卷第6期,共3页

基　　金：上海交通大学首批农科合作基金;国际校际合作交流项目~~ 摘　　要：The recombinant suicide plasmid pYYvpr contained a Kan resistant cassette and a 778bp internal piece of the vpr gene. It was transformed into the wild type E.coli strain, MC 1061,which possessed the full-length vpr gene and did not contain the λ pir gene. The recombinant suicide plasmid could not replicate in MC 1061. Colonies grew on the plates of Kan were analysed by PCR and Southern blot with two different Dig-probes,vpr and Kan R probes. One colony was detected vpr and Kan R gene by PCR and Southern blot showed the mutant had the different vpr map with the wild type. The isogenic knockout mutant, named MC1061 Δvpr,was confirmed.The mutant supported lysogenic infection of VT2 recombinant phage, but did not support lytic infection. All results showed that the vpr gene should be related to the lytic infection of VT2 phage. 关键词：VT噬菌体受体 vpr基因突变鉴定大肠杆菌0157 强致病性

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大肠杆菌VT噬菌体受体(vpr)基因突变株的鉴定

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