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Promoter and coding sequence diversity of CsCCD1 may contribute to the differential accumulation of floralβ-ionone in fresh tea leaves

查看全文 作　　者：Jingming [1]Wang;Mingyue [1]Zhao;Ting [1]Gao;Yingying [1]Feng;Feiquan [2]Wang;Yuting [1]Pan;Jieyang [1]Jin;Tingting [1]Jing;Mengqian [1]Lu;Mengting [1]Zhang;Danyang [1]Guo;Xiaochun [1]Wan;Wilfried [1,3]Schwab;Chuankui [1]Song 高影响力作者 机构地区：[1]State Key Laboratory of Tea Plant Biology and Utilization,International Joint Laboratory on Tea Chemistry and Health Effects,Anhui Agricultural University,Hefei,Anhui 230036,China;[2]Wuyi University,Wuyishan,Fujian 354300,China;[3]Biotechnology of Natural Products,Technische Universität München,Liesel-Beckmann-Str.1,Freising 85354,Germany高影响力机构 出　　处：《Horticultural Plant Journal》索引2023年第9卷第4期,共11页高影响力期刊 基　　金：financially supported by National Natural Science Foundation of China(Grant Nos.31961133030,31870678,32022076);Science Fund for Distinguished Young Scientists of Anhui Province(Grant No.1908085J12). 摘　　要：The carotenoid-derived volatileβ-ionone makes an important contribution to tea fragrance.Here,we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS.The 13 cultivars were divided into two groups by PCA(Principal Component Analysis)clustering analysis of their carotenoid content,and OPLS-DA(Orthogonal projections to latent structures)indicated that the levels ofβ-carotene(VIP=2.89)and lutein(VIP=2.30)were responsible for much of the variation between the two groups.Interestingly,theβ-carotene toβ-ionone conversion rates in Group 1 were higher than in Group 2,while theβ-carotene content was significantly lower in Group 1 than in Group 2.Theβ-ionone content was significantly higher in Group 1.Pearson Correlation Coefficient calculation between the transcription level of candidate genes(CsCCD1 and CsCCD4)and the accumulation ofβ-ionone indicated that CsCCD1 may involve in the formation ofβ-ionone in 13 cultivars.Prokaryotic expression and in vitro enzyme activity assays showed that‘Chuanhuang 1’had an amino acid mutation in carotenoid cleavage dioxygenases 1(CsCCD1)compared with‘Shuchazao’,resulting in a significantly higherβ-ionone content in‘Chuanhuang 1’.Sequence analysis showed that‘Chuanhuang 1’and‘Huangdan’had different CsCCD1 promoter sequences,leading to significantly higher CsCCD1 expression andβ-ionone accumulation in‘Chuanhuang 1’.These results indicated that the promoter and coding sequence diversity of CsCCD1 might contribute to the differential accumulation ofβ-ionone in different tea cultivars. 关 键 词：Camellia sinensis(L.)Kuntze Carotenoid cleavage dioxygenase Β-CAROTENE Β-IONONE PROMOTER Differential accumulation
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