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Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma

查看全文 作　　者：Bin [1]LUO;Xiang [2]YUN;Jing [3]LI;Rong [4]FAN;Wen-wen [5]GUO;Chang [2]LIU;Yong-da [1]LIN;Ying-ying [1]GE;Xia [1]ZENG;Shui-qing [1]BI;Wei-xia [1]NONG;Qing-mei [1]ZHANG;Xiao-xun [1,6,7]XIE 高影响力作者 机构地区：[1]School of Basic Medical Sciences,Guangxi Medical University,Nanning 530021,China;[2]Department of Surgery,the First Affiliated Hospital,Guangxi Medical University,Nanning 530021,China;[3]Clinical Laboratory,Chinese Medicine Hospital,Quanzhou 362000,China;[4]School of Basic Medical Sciences,Guangxi University of Chinese Medicine,Nanning 530021,China;[5]Department of Pathology,the People's Hospital of Guangxi Zhuang Autonomous Region,Nanning 530021,China;[6]Guangxi Key Laboratory of Early Prevention and Treatmentfor Regional High Frequency Tumor,Guangxi Medical University,Nanning 530021,China;[7]Key Laboratory Research on Preclinical Medicine,Guangxi Medical University,Nanning 530021,China高影响力机构 出　　处：《Current Medical Science》索引2020年第40卷第4期,共10页高影响力期刊 基　　金：This work was supported by grants from the National Natural Science Foundation of China(No.81860445,No.81960453,No.81560408,and No.81660429);Natural Science Foundation of Guangxi Province(No.2016GXNSFBA380159,No.2018GXNSFAA281251,No.2018GXNSFAA050151,No.2017GXNSFAA198001,No.2018GXNSFAA281050,and No.2018GXNSFBA281187);Key Laboratory ofEarly Prevention and Treatment for Regional High Frequency Tumor(Guangxi Medical University);Ministry ofEducation(No.GK2018-09,No.GKE 2019-08,and No.K2015-TKF03);Basic Ability Improvement Project for Young and Middle-aged Teachers in Colleges and Universities of Guangxi Province(No.2018KY0109). 摘　　要：Summary:Cancer testis(CT)antigens have received particular attention in cancer immunotherapy.OY-TES-1 is a member of CT antigens.This study was to evaluate OY-TES-1 expression and immunogenicity in hepatocelluar carcinoma(HCC).OY-TES-1 mRNA expression was detected in 56 HCC tissues and 5 normal liver tissues by reverse transcriptase PCR(RT-PCR).Of the 56 cases of HCC tissues tested,37 cases had tumor and matched adjacent non-cancer tissues and were subjected to both RT-PCR and quantitative real-time PCR.OY-TES-1 protein was subsequently observed on a panel of tissue microarrays.Sera from patients were tested for OY-TES-1 antibody by ELISA.To identify OY-TES-1 capable of inducing cellular immune response,OY-TES-1 protein was used to sensitize dentritic cells and the cytotoxicity effect was measured in vitro.The results showed that OY-TES-1 mRNA was highly expressed in 41 of the 56(73.21%)HCC tissues,whereas none in 5 normal liver tissues.OY-TES-1 mRNA was frequently expressed not only in HCC tissues(72.97%,27/37),but also in paired adjacent non-cancer tissues(64.86%,24/37).But the mean expression level of OY-TES-1 mRNA in HCC tissues was significantly higher than that in adjacent non-cancer tissues(0.76854 vs.0.09834,P=0.021).Immunohistochemistry showed that OY-TES-1 protein expression was detected in 6 of the 49 cases of HCC tissues,and absent in 9 cases of normal liver and 6 cases of cirrhosis tissues.Seropositivity was detected in 10 of the 45 HCC patients,but not detected in 17 cirrhosis patients and 76 healthy donors.The specific cytotoxic T cells elicited by OY-TES-1 could kill HLA-A2^+HCC cell line which expressed OY-TES-1.The target lysis was mainly HLA class I-dependent and could be blocked by antibodies against monomorphic HLA class I but not HLA class II molecule.In summary,OY-TES-1 expression is upregulated in HCC tissues and can be recognized by humoral and cellular responses,which suggests that OY-TES-1 is an attractive target for tumor immunotherapy in HCC. 关 键 词：cancer testis antigen hepatocelluar cancer tumor immunotherapy
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