Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli: Culturability, membrane permeability,metabolism, and genetic damage

查看全文 作　　者：Limei [1]Xu;Chongmiao [2]Zhang;Pengcheng [3]Xu;Xiaochang [4]C.Wang

机构地区：[1]International Science and Technology Cooperation Center for Urban Alternative Water Resources Development, China;[2]Key Laboratory of Northwest Water Resource, Environment and Ecology, MOE, China;[3]Engineering Technology Research Center for Wastewater Treatment and Reuse, Shaanxi Province, China;[4]School of Environmental and Municipal Engineering, Xi'an University of Architecture and Technology, Xi'an 710055, China

出　　处：《Journal of Environmental Sciences》索引2018年第30卷第3期,共11页

基　　金：supported by the National Natural Science Foundation of China (No. 51578441);the National Program of Water Pollution Control (No. 2013ZX07310-001);the Scientific Research Program funded by the Shaanxi Provincial Education Department (No. 15JK1442);the National Key Technology Support Program (No. 2014BAC13B06);the Program for Innovative Research Team in Shaanxi Province (No. 2013KCT-13) 摘　　要：Traditional culture methods may underestimate the tolerance of microorganisms to disinfectants because of the existence of viable but nonculturable or sublethally injured cells after disinfection.The selection of a strict method is crucial for the evaluation of disinfection performance.The actions of 2 typical disinfectants–ultraviolet(UV)and chlorine–on the fecal indicator Escherichia coli were investigated by the detection of culturability,membrane permeability,metabolic activity,deoxyribonucleic acid(DNA),and messenger ribonucleic acid(m RNA).During UV disinfection,the irreversible damages in the cell membrane and cellular adenosine triphosphate(ATP)were negligible at low UV doses(<80 m J/cm^2).However,membrane permeability was damaged at low doses of chlorine(<5 mg/L),leading to leakage of cellular ATP.Our study showed that a slight lesion in DNA was detected even at high doses of UV(400 m J/cm^2)and chlorine(>5 mg/L)treatments.The decay of m RNA was more rapid than that of DNA.The degradation level of m RNA depended on the choice of target genes.After exposure to 50 m J/cm^2UV dose or 5 mg/L chlorine for30 min,the DNA damage repair function(Rec A m RNA)was inhibited.The m RNA involved in the DNA damage repair function can be a potential indicator of bacterial viability. 关键词：紫外消毒新陈代谢膜渗透氯化机制损坏基因脱氧核糖核酸

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Mechanisms of ultraviolet disinfection and chlorination of Escherichia coli: Culturability, membrane permeability,metabolism, and genetic damage

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