Human cytomegalovirus miR-US5-1 inhibits viral replication by targeting Geminin mRNA

查看全文 作　　者：Shujuan [1,2]Jiang;Yujing [1]Huang;Ying [1]Qi;Rong [2]He;Zhongyang [1]Liu;Yanping [1]Ma;Xin [1]Guo;Yaozhong [1]Shao;Zhengrong [1]Sun;Qiang [1]Ruan

机构地区：[1]Virus Laboratory, the Affiliated Shengjing Hospital, China Medical University, Shenyang 110122, China;[2]Clinical Genetics, the Affiliated Shengjing Hospital, China Medical University, Shenyang 110122, China

出　　处：《Virologica Sinica》索引2017年第32卷第5期,共9页

基　　金：supported by the National Natural Science Foundation of China (81371788 and 81171580);the Specialized Research Fund for the Doctoral Program of Higher Education (20112104110012);the Outstanding Scientific Fund of Shengjing Hospital 摘　　要：Viruses commonly create favorable cellular conditions for their survival through multiple mechanisms. Micro RNAs(mi RNAs), which function as post-transcriptional regulators, are utilized by human cytomegalovirus(HCMV) in its infection and pathogenesis. In the present study, the DNA replication inhibitor Geminin(GMNN) was identified to be a direct target of hcmv-mi R-US5-1. Overexpression of hcmv-mi R-US5-1 could block the accumulation of GMNN during HCMV infection, and the decrease of GMNN expression caused by hcmv-mi R-US5-1 or GMNN specific si RNA reduced HCMV DNA copies in U373 cells. Meanwhile, ectopic expression of hcmv-mi R-US5-1 and consequent lower expression of GMNN influenced host cell cycle and proliferation. These results imply that hcmv-mi R-US5-1 may affect viral replication and host cellular environment by regulating expression kinetics of GMNN during HCMV infection. 关键词：Human cytomegalovirus(HCMV) hcmv-mi R-US5-1 Geminin(GMNN) DNA replication cell cycle

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Human cytomegalovirus miR-US5-1 inhibits viral replication by targeting Geminin mRNA

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