FT Ⅰ, a novel positive myeloid-lineage-specific transcription regulatory element within the mouse myeloperoxidase gene enhancer, En 1

查看全文 作　　者：ZHU JINGDE (CRC Department of Medical Oncology, CRC Beat son Laboratories, Glasgow University, Garscube Estate, Switchback Road, Glasgow G61 1BD, United Kngdom. Tel: 44141942 9361;Fax: 44141 330 4127. e-mail:gpma66@udcf. gla. ac. uk ) 出　　处：《Cell Research》索引1995年第5卷第1期,共17页 摘　　要：FT Ⅰ (AAAAGGGGAAGCAGAG), a poly purine ele-ment within the myloid-lineage specific enhancer (En 1) of the mouse myeloperoxidase gene [1, 2] has been fur-ther characterised. 1, FT Ⅰ functions as a myeloid-lineage specific transcription regulatory element; 2, WEHI 3BD+ cells have higher binding activity to FT Ⅰ and express the proteins which could form the unique DNA-protein com-plex(es) of FT Ⅰ;. 3, The essential sequence for the specific DNA-protein interactions of FT Ⅰ is AAAAGGGGAAGC; 4, South-western analysis in conjunction with the compe-tition assay of the proteins binding to FT Ⅰ, has revealed a 28 kd protein in WEHI 3BD+ cells that displays the properties of the putative transcription factor which acts through FT Ⅰ. These new findings have demonstrated both the functional myeloid-lineage specificity and the novelty of FT Ⅰ. 关 键 词：FT I EN 1 鼠骨髓过氧化酶基因 多嘌呤元件 转录活化 增强因子