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Expression and function of transcription factor RUNX2 in synovial tissues of rheumatoid arthritis

查看全文 作　　者：Jin [1]Zhang;Xinhu [1]Wang;Xiajun [1]Liu;Yuanchi [1]Huang;Qing [1]She 高影响力作者 机构地区：[1]Baoji Center Hospital,Baoji 721008,China高影响力机构 出　　处：《广西医科大学学报》索引2017年第34卷第10期,共5页高影响力期刊 基　　金：supported by Science and Technology Research Project in Shaanxi province(No.2014K11-03-06-10) 摘　　要：Objective:This study aimed to explore the expression profile of transcription factor Runt-related transcription factor 2(RUNX2)in the synovial tissues of rheumatoid arthritis(RA)and its effect on the apoptosis of RA fibroblast-like synovial cells(RA-FLS),and to screen new targets for the diagnosis,treatment and prognosis of RA.Methods:The expression and localization of mRNA and protein of RUNX2 in the synovial tissues were detected by real-time quantitative PCR(q PCR)and immunohistochemical staining,respectively.The effect of the expression of exogenous RUNX2 on the apoptosis process of RA-FLS cell line MH7A was investigated by flow cytometry,and the activation of NF-κB signaling pathway was detected by western blotting.Results:The expression of RUNX2 mRNA was significantly higher in the synovial tissues from RA patients,compared to that in the OA patients(P<0.05).RUNX2 protein was mainly localized in the nuclear of the RA synovial cells.Overexpression of exogenous RUNX2 could notably decrease the apoptosis of RA-FLS,which was substantially reversed by pretreatment with SN50,a specific inhibitor of NF-κB pathway.Compared with blank group and negative control group(p CMV6-AC-GFP-vector),the apoptotic rate of exogenous RUNX2 overexpressing(pCMV6-AC-GFP-RUNX2)MH7A cells was significantly decreased(P<0.05).NF-κB signaling pathway activity was significantly increased(P<0.05),and this effect could be effectively reversed by NF-κB signal pathway-specific inhibitor SN5.Conclusion:The high expression of UNX2 in RA synoviocytes could significantly inhibit the spontaneous apoptosis of cells,and it was related to the abnormal activation of NF-κB signaling pathway.In-depth studies of RUNX2/NF-κB signaling pathways will help to identify novel therapeutic targets for RA. 关 键 词：医疗卫生行业 医学研究 临床治疗 患者
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