Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs

查看全文 作　　者：Bo [1]Jiang;Zhang-You [1]Wu;Zi-Cheng [1]Zhu;Gen-Jie [1]Ke;Yue-Chun [1]Wen;Si-Qin [1]Sun

机构地区：[1]Department of Ophthalmology,Anhui Provincial Hospital,Anhui Medical University

出　　处：《International Journal of Ophthalmology(English edition)》索引2017年第10卷第4期,共5页

基　　金：Supported by the Natural Science Foundation of Anhui Province(No.1508085MH188);Science Foundation of Anhui Provincial Health Bureau(No.13zc040;No.13zc046) 摘　　要：AIM:To study the expression of collagen I and transcription factor specificity protein 1(Sp1),a transforming growth factor-β1(TGF-β1) downstream target,and reveal the impact of the TGF-β1-Sp1 signaling pathway on collagen remodeling in myopic sclera.METHODS:Seventy-five 1-week-old guinea pigs were randomly divided into normal control,form deprivation myopia(FDM),and self-control groups.FDM was induced for different times using coverage with translucent latex balloons and FDM recovery was performed for 1wk after 4wk treatment;then,changes in refractive power and axial length were measured.Immunohistochemistry and reverse transcription-polymerase chain reaction were used to evaluate dynamic changes in collagen I and Sp1 expression in the sclera of guinea pigs with emmetropia and experimental myopia,and the relationship between collagen I and Sp1 levels was analyzed.RESULTS:In the FDM group,the refractive power was gradually changed(from 2.09±0.30 D at week 0 to-1.23±0.69 D,-4.17±0.59 D,-7.07±0.56 D,and-4.30±0.58 D at weeks 2,4,6,and 1wk after 4wk,respectively;P<0.05),indicating deepening of myopia.The axial length was increased(from 5.92±0.39 mm at week 0 to 6.62±0.36 mm,7.30±0.34 mm,7.99±0.32 mm,and 7.41±0.36 mm at weeks 2,4,6,and 1wk after 4wk;P<0.05).The m RNA and protein expression of Sp1 and collagen I in the sclera of the FDM group was lower than that of the control groups(P<0.05),and the reduction was eye-coverage time-dependent.Furthermore,correlation between Sp1 and collagen I down-regulation in the myopic sclera was observed.CONCLUSION:Our data indicate that transcription factor Sp1 may be involved in the regulation of type I collagensynthesis/degradation during myopic sclera remodeling,suggesting that TGF-β1 signaling plays a role in the development and progression of myopia. 关键词：形式剥夺近视改变的巩膜转变生长 factor-1 特性蛋白质 1 骨胶原我

相关文献

参考文献（20）
引证文献（16）
耦合文献（20）

参考文献(20)

引证文献(16)

耦合文献(20)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Expression and role of specificity protein 1 in the sclera remodeling of experimental myopia in guinea pigs

参考文献(20)

引证文献(16)

耦合文献(20)

用户提示