Key genes expressed in different stages of spinal cord ischemia/reperfusion injury ischemia/reperfusion injury

查看全文 作　　者：lian-an [1]Li;Chun-fang [1]Zan;Peng [1]Xia;Chang-jun [1]Zheng;Zhi-ping [1]Qi;Chun-xu [1]Li;Zhi-gang [1]Liu;Ting-ting [1]Hou;Xiao-yu [1]Yang 机构地区：[1]Department of Orthopaedics, the Second Hospital of Jilin University, Changchun, Jilin Province, China 出　　处：《Neural Regeneration Research》索引2016年第11卷第11期,共6页 基　　金：supported by a Grant from the National Natural Science Foundation of China,No.81350013,31572217,and 81672263 摘　　要：The temporal expression of microRNA after spinal cord ischemia/reperfusion injury is not yet fully understood.In the present study,we established a model of spinal cord ischemia in Sprague-Dawley rats by clamping the abdominal aorta for 90 minutes,before allowing reperfusion for 24 or 48 hours.A sham-operated group underwent surgery but the aorta was not clamped.The damaged spinal cord was removed for hematoxylin-eosin staining and RNA extraction.Neuronal degeneration and tissue edema were the most severe in the 24-hour reperfusion group,and milder in the 48-hour reperfusion group.RNA amplification,labeling,and hybridization were used to obtain the microRNA expression profiles of each group.Bioinformatics analysis confirmed four differentially expressed microRNAs(miR-22-3p,miR-743b-3p,miR-201-5p and miR-144-5p) and their common target genes(Tmem69 and CxcllO).Compared with the sham group,miR-22-3p was continuously upregulated in all three ischemia groups but was highest in the group with no reperfusion,whereas miR-743b-3p,miR-201-5p and miR-144-5p were downregulated in the three ischemia groups.We have successfully identified the key genes expressed at different stages of spinal cord ischemia/reperfusion injury,which provide a reference for future investigations into the mechanism of spinal cord injury. 关 键 词：缺血再灌注损伤 脊髓损伤 靶基因 microRNA 缺血/再灌注损伤 腹主动脉 差异表达 生物信息