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Effects of Storage Temperature on the Effective Activity of Straw Frozen Semen after Thawing

查看全文 作　　者：Qiao [1]Limin;Ma [1]Jianmin;Qiao [2]Fuqiang;Yao [3]Hua;Xiao [1]Xishan;Hou [1]Yinxu;Fu [1]Jingtao;Ren [4]Kang 高影响力作者 机构地区：[1]Beijing Vocational College of Agriculture;[2]Technology Industry Group.BUA;[3]Animal Science and Technology College,Beijing University of Agriculture;[4]Beijing Municipal Bureau of Agriculture高影响力机构 出　　处：《Animal Husbandry and Feed Science》索引2015年第7卷第4期,共3页高影响力期刊 基　　金：Supported by the Technology Research and Demonstrational Popularization Project of Beijing Vocational College of Agriculture(XY-YF-15-07)(XY-YF-14-21) 摘　　要：The aim was to discuss the optimal storage environment and proper insemination time after thawing of 0. 25 m L straw frozen semen. Straw frozen semen was thawed at 40 ℃ for 20 s,and then stored at 0- 4 ℃,14- 16 ℃,25- 27 ℃ for 2,4,6,8 and 10 h,respectively. The sperm motility was detected. After thawing,semen was stored at 0- 4 ℃ and 14- 16 ℃ for 10 h. Their sperm motilities( 0. 434 ± 0. 016 7 and 0. 423 ± 0. 019 6) had no significant differences( P> 0. 05) with initial thawing motility( 0. 441 ± 0. 030). Sperm motility reduced as the storage time prolonged at 25- 27 ℃. Sperm motility after 6 h had significant differences with that of initial thawing motility( P < 0. 05),and sperm motilities after 8 and 10 h showed extremely significant differences( P < 0. 01).Thus,sperm motility after thawing was still very high after stored at 0- 4 ℃ and 14- 16 ℃ within 10 h,which met the requirements for insemination. Under this temperature and time ranges,sperm could be carried over long distances,which had small effects on sperm quality and reached the expected insemination effects.However,under the temperature of 25- 27 ℃,semen should be used for insemination within 6 h after thawing. 关 键 词：精液解冻 冷冻精液 贮藏温度 精子运动能力 精子活力 活性 人工授精 授精时间
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