Cidea controls lipid droplet fusion and lipid storage in brown and white adipose tissue

查看全文 作　　者：WU [1]LiZhen;ZHOU [1]LinKang;CHEN [2]Cheng;GONG [1]JingYi;XU [3]Li;YE [4]Jing;LI [5]De;LI [1]Peng

机构地区：[1]MOE key laboratory of Bioinformatics and Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University;[2]Department of Biology, Hong Kong University of Science and Technology;[3]Key Laboratory for Feed Biotechnology of the Ministry of Agriculture,Feed Research Institute, Chinese Academy of Agricultural Sciences;[4]Department of Pathology, The Fourth Military Medical University;[5]Center of Biomedical Analysis, Tsinghua University

出　　处：《Science China(Life Sciences)》索引2014年第57卷第1期,共10页

基　　金：supported by grants from the National Basic Research Program(2013CB530602 and 2011CB910801 to Li Peng);the National Natural Science Foundation of China(30925017,31030038 and 90913024);the China Postdoctoral Science Foundation(2012M520249 and 2013T60103 to Zhou LinKang) 摘　　要：Excess lipid storage in adipose tissue results in the development of obesity and other metabolic disorders including diabetes,fatty liver and cardiovascular diseases.The lipid droplet(LD)is an important subcellular organelle responsible for lipid storage.We previously observed that Fsp27,a member of the CIDE family proteins,is localized to LD-contact sites and promotes atypical LD fusion and growth.Cidea,a close homolog of Fsp27,is expressed at high levels in brown adipose tissue.However,the exact role of Cidea in promoting LD fusion and lipid storage in adipose tissue remains unknown.Here,we expressed Cidea in Fsp27-knockdown adipocytes and observed that Cidea has similar activity to Fsp27 in promoting lipid storage and LD fusion and growth.Next,we generated Cidea and Fsp27 double-deficient mice and observed that these animals had drastically reduced adipose tissue mass and a strong lean phenotype.In addition,Cidea/Fsp27 double-deficient mice had improved insulin sensitivity and were intolerant to cold.Furthermore,we observed that the brown and white adipose tissues of Cidea/Fsp27double-deficient mice had significantly reduced lipid storage and contained smaller LDs compared to those of Cidea or Fsp27single deficient mice.Overall,these data reveal an important role of Cidea in controlling lipid droplet fusion,lipid storage in brown and white adipose tissue,and the development of obesity. 关键词：棕色脂肪组织脂质储存控制胰岛素敏感性心血管疾病代谢紊乱细胞表达

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Cidea controls lipid droplet fusion and lipid storage in brown and white adipose tissue

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