Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress

查看全文 作　　者：Lei [1,2]Zhao;Mei [1]Chen;Dongmei [1,2]Cheng;Haomeng [1]Yang;Yongle [1]Sun;Heyi [1,2]Zhou;Fang [1]Huang

机构地区：[1]Key Laboratory of Photobiology, Institute of Botany, the Chinese Academy of Sciences;[2]University of Chinese Academy of Sciences

出　　处：《Journal of Integrative Plant Biology》索引2013年第55卷第11期,共15页

基　　金：supported by the Ministry of Science and Technology of China (2009CB220000);Chinese Ministry of Agriculture (2009ZX08009-121B);the Chinese Academy of Sciences 摘　　要：The genome of unicellular green alga Chlamydomonas reinhardtii contains four genes encoding B-type methionine sulfoxide reductases,MSRB1.1,MSRB1.2,MSRB2.1,and MSRB2.2,with functions largely unknown.To understand the cell defense system mediated by the methionine sulfoxide reductases in Chlamydomonas,we analyzed expression and physiological roles of the MSRBs under different abiotic stress conditions using immunoblotting and quantitative polymerase chain reaction(PCR)analyses.We showed that the MSRB2.2 protein was accumulated in cells treated with high light(1,300 mE/m2per s),whereas MSRB1.1 was accumulated in the cells under 1 mmol/L H2O2treatment or sulfur depletion.We observed that the cells with the MSRB2.2 knockdown and overexpression displayed increased and decreased sensitivity to high light,respectively,based on in situ chlorophyll a fuorescence measures.We also observed that the cells with the MSRB1.1 knockdown and overexpression displayed decreased and increased tolerance to sulfur-depletion and oxidative stresses,respectively,based on growth and H2-producing performance.The physiological implications revealed from the experimental data highlight the importance of MSRB2.2 and MSRB1.1 in protecting Chlamydomonas cells against adverse conditions such as high-light,sulfur-depletion,and oxidative stresses. 关键词：蛋氨酸亚砜莱茵衣藻氧化应激还原酶含硫保护 B型单细胞绿藻

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Different B-Type Methionine Sulfoxide Reductases in Chlamydomonas May Protect the Alga against High-Light, Sulfur-Depletion, or Oxidative Stress

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